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Segmental labeling of the intrinsically disordered protein PQBP1 and elucidation of the molecular recognition mechanism by NMR

Research Project

Project/Area Number 21790034
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionUniversity of Toyama

Principal Investigator

MIZUGUCHI Mineyuki  富山大学, 大学院・医学薬学研究部(薬学), 教授 (30332662)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords生体分子 / 蛋白質 / 分子認識 / NMR / タンパク質 / 天然変性蛋白質
Research Abstract

We successfully prepared segmentally labeled PQBP1, in which the C-terminal domain is 15N-labeled. Two fragments of PQBP1 were highly expressed in E. coli with the cold-shock vector, and ligated by the expressed protein ligation method. Our results will be applicable to the segmental labeling of other intrinsically disordered proteins.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (23 results)

All 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 8 results) Presentation (12 results) Remarks (1 results)

  • [Journal Article] Hydrogen-bondnetwork and pH sensitivity in transthyretin : neutron crystal structure of humantransthyretin2012

    • Author(s)
      Yokoyama, T., Mizuguchi, M., Nabeshima, Y., Kusaka, K., Yamada, T., Hosoya, T., Ohhara, T., Kurihara, K., Tomoyori, K., Tanaka, I. and Niimura. N.
    • Journal Title

      J Struct Biol

      Volume: 177 Pages: 283-290

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hydrogen-bond network and pH sensitivity in transthyretin: neutron crystal structure of human transthyretin2012

    • Author(s)
      Yokoyama, T., et al.
    • Journal Title

      Journal of Structural Biology

      Volume: 177 Pages: 283-290

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 天然変性タンパク質PQBP-1の揺らぎと生体機能.メディカルバイオ2010

    • Author(s)
      水口峰之, 岡澤均
    • Journal Title

      10月号別冊「揺らぎと生体機能」

      Pages: 44-48

    • Related Report
      2011 Final Research Report
  • [Journal Article] Polyglutamine tract-bindingprotein-1 binds to U5-15kD via a continuous23-residue segment of the C-terminaldomain2010

    • Author(s)
      Takahashi, M., Mizuguchi, M., Shinoda, H., Aizawa, T., Demura, M., Okazawa H. andKawano, K
    • Journal Title

      Biochim Biophys Acta

      Volume: 1804 Pages: 1500-1507

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polyglutamine tract-binding protein-1 binds to U5-15kD via a continuous 23-residue segment of the C-terminal domain.2010

    • Author(s)
      Takahashi, M., et al.
    • Journal Title

      Biochimica et Biophysica Acta-Proteins and Proteomics

      Volume: 1804 Pages: 1500-1507

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 天然変性タンパク質PQBP-1の揺らぎと生体機能2010

    • Author(s)
      水口峰之, 岡澤均
    • Journal Title

      メディカルバイオ

      Volume: 10月号別冊 Pages: 44-48

    • Related Report
      2010 Annual Research Report
  • [Journal Article] A novelβ-defensin structure : big defensin changes its N-terminal structureto associate with the target membrane2009

    • Author(s)
      Kouno, T., Mizuguchi, M., Aizawa, T., Shinoda, H., Demura, M., Kawabata, S. andKawano, K
    • Journal Title

      Biochemistry 48

      Pages: 7629-7635

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polyglutamine tract bindingprotein-1 is an intrinsically unstructuredprotein2009

    • Author(s)
      Takahashi, M., Mizuguchi, M., Shinoda, H., Aizawa, T., Demura, M., Okazawa, H. andKawano, K
    • Journal Title

      Biochim Biophys Acta

      Volume: 1794 Pages: 936-943

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polyglutamine tract binding protein-1 in an intrinsically unstructured protein.2009

    • Author(s)
      Takahashi, M., et al.
    • Journal Title

      Biochimica et Biophysica Acta-Proteins and Proteomics 1794

      Pages: 936-943

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel β-defensin structure : big defensin changes its N-terminal structure to associate with the target membrane.2009

    • Author(s)
      Kouno, T., et al.
    • Journal Title

      Biochemistry 48

      Pages: 7629-7635

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] ポリグルタミン結合タンパク質の構造生物学的研究2012

    • Author(s)
      水口峰之
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      2012-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] ポリグルタミン結合タンパク質の構造生物学的研究2012

    • Author(s)
      水口峰之
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(招待講演)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structural changes of theintrinsically disordered protein PQBP-12012

    • Author(s)
      Mizuguchi, M
    • Organizer
      9thJapan-Korea Bilateral Symposium onBiological NMR
    • Place of Presentation
      Sapporo
    • Year and Date
      2012-03-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Structural changes of the intrinsically disordered protein PQBP-12012

    • Author(s)
      Mizuguchi M.
    • Organizer
      9th Japan-Korea Bilateral Symposium on Biological NMR
    • Place of Presentation
      Hokkaido University(招待講演)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dynamic structural changesof the intrinsically disordered proteinPQBP-12012

    • Author(s)
      Mizuguchi, M
    • Organizer
      4th Japan-Korea Seminar onBiomolecular Sciences, Experiments andSimulations
    • Place of Presentation
      Nara
    • Year and Date
      2012-01-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Dynamic structural changes of the intrinsically disordered protein PQBP-12012

    • Author(s)
      Mizuguchi M.
    • Organizer
      4th Japan-Korea Seminar on Biomolecular Sciences, Experiments and Simulations
    • Place of Presentation
      Cultural Center of Todaiji-Temple(招待講演)
    • Year and Date
      2012-01-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 精神遅滞の原因タンパク質PQBP-1の構造と機能2011

    • Author(s)
      水口峰之
    • Organizer
      第21回WSフォーラムタンパク質・ペプチド研究の現状と展望
    • Place of Presentation
      福岡
    • Year and Date
      2011-11-19
    • Related Report
      2011 Final Research Report
  • [Presentation] 精神遅滞の原因タンパク質PQBP-1の構造と機能2011

    • Author(s)
      水口峰之
    • Organizer
      第21回WSフォーラムタンパク質・ペプチド研究の現状と展望
    • Place of Presentation
      九州大学(招待講演)
    • Year and Date
      2011-11-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] 天然変性蛋白質PQBP-1の動的な構造変化2011

    • Author(s)
      水口峰之
    • Organizer
      物理学会2011年秋季大会
    • Place of Presentation
      富山
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] 天然変性蛋白質PQBP-1の動的な構造変化2011

    • Author(s)
      水口峰之
    • Organizer
      物理学会2011年秋季大会
    • Place of Presentation
      富山大学(招待講演)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Fluctuation and function ofpolyglutamine tract binding protein-12009

    • Author(s)
      Mizuguchi, M., Takahashi, M., Okazawa, H. and Kawano, K
    • Organizer
      The3rd International Symposium on MolecularScience of Fluctuations toward BiologicalFunctions
    • Place of Presentation
      Nagoya
    • Year and Date
      2009-12-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Fluctuation and function of polyglutamine tract binding protein-12009

    • Author(s)
      Mizuguchi, M., Takahashi, M., Okazawa, H., Kawano, K.
    • Organizer
      The 3rd International Symposium on Molecular Science of Fluctuations toward Biological Functions
    • Place of Presentation
      Nagoya University
    • Year and Date
      2009-12-21
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.pha.u-toyama.ac.jp/phypha2/index-j.html

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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