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a Novel Screening System for Potential Anticancer Drugs with Gene Amplification Method

Research Project

Project/Area Number 21790045
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionTokyo University of Science

Principal Investigator

AKIRA Inoue  東京理科大学, 薬学部, 嘱託助教 (30516406)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords遺伝子増幅 / コンビナトリアルケミストリー / 金コロイド粒子 / DNA結合分子 / スクリーニング / 金ナノ粒子 / プライマーダイマー(PDs) / リアルタイムPCR / PCR / プライマーダイマー / 抗がん剤 / DNA相互作用分子
Research Abstract

DNA-binding molecules are potential anticancer drugs to regulate gene amplification or gene expressions. The accurate relative determination of DNA-binding affinities of chemical compounds is an important factor for drug discovery area. In this work, I have developed a novel system to evaluate relative DNA-binding affinities of chemical compounds with primer-dimer(PD) templates using a real-time PCR analyzer. The utility of PD templates allows us to be accurate and highly sensitive determination of DNA-binding affinities of chemical compounds without the amplification of non-specific PCR products. We also developed the colorimetric determination system of DNA-binding affinities of chemical compounds in combination with gold nanoparticle aggregation phenomena and PCR-based evaluation system described above.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report

Research Products

(8 results)

All 2011 2010 2009

All Journal Article Presentation

  • [Journal Article] Accurate and Highly Sensitive Screening DNA-binding Molecules with Primer-dimer Templates Using a Real-time PCR Analyzer2011

    • Author(s)
      Akira Inoue, Tsunehiro Kumeda, and Hiroshi Nakamrua
    • Journal Title

      Analytical Sciences

      Volume: 27 Pages: 461-463

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Accurate and Highly Sensitive Screening of DNA-binding Molecules with primer-dimer Templates Using a Real-time PCR analyzer2011

    • Author(s)
      Akira Inoue, Tsunehiro Kumeda, Hiroshi Nakamura
    • Journal Title

      Analytical Sciences

      Volume: 27 Pages: 461-463

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] DNA相互作用性に基づく抗がん剤のスクリーニング法2010

    • Author(s)
      粂田恒宏、井上明、中村洋
    • Organizer
      第70回分析化学討論会
    • Place of Presentation
      島根大学松江キャンパス
    • Year and Date
      2010-05-15
    • Related Report
      2011 Final Research Report
  • [Presentation] PCRによるDNA相互作用性分子の検出法~金コロイド粒子による可視化~2010

    • Author(s)
      井上明、粂田恒宏、中村洋
    • Organizer
      第14回LCテクノプラザ
    • Place of Presentation
      東京理科大学
    • Year and Date
      2010-01-28
    • Related Report
      2011 Final Research Report
  • [Presentation] PCRによるDNA相互作用性分子の検出法~金コロイド粒子による可視化~2010

    • Author(s)
      井上明
    • Organizer
      第15回LCテクノプラザ講演要旨集
    • Place of Presentation
      東京理科大学
    • Year and Date
      2010-01-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] DNA相互作用分子の新たな評価方法"PCR-Screening Assay"2009

    • Author(s)
      井上明、粂田恒弘、中村洋
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-26
    • Related Report
      2011 Final Research Report
  • [Presentation] DNA相互作用分子の新たな評価方法;PCR-Screening Assay2009

    • Author(s)
      井上明
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] PCRを用いた抗がん剤のスクリーニング法2009

    • Author(s)
      粂田恒宏、井上明、中村洋
    • Organizer
      第70回分析化学討論会
    • Place of Presentation
      和歌山大学栄谷キャンパス
    • Year and Date
      2009-05-16
    • Related Report
      2011 Final Research Report

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Published: 2009-03-31   Modified: 2016-04-21  

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