a Novel Screening System for Potential Anticancer Drugs with Gene Amplification Method
| Project/Area Number |
21790045
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
AKIRA Inoue 東京理科大学, 薬学部, 嘱託助教 (30516406)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 遺伝子増幅 / コンビナトリアルケミストリー / 金コロイド粒子 / DNA結合分子 / スクリーニング / 金ナノ粒子 / プライマーダイマー(PDs) / リアルタイムPCR / PCR / プライマーダイマー / 抗がん剤 / DNA相互作用分子 |
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| Research Abstract |
DNA-binding molecules are potential anticancer drugs to regulate gene amplification or gene expressions. The accurate relative determination of DNA-binding affinities of chemical compounds is an important factor for drug discovery area. In this work, I have developed a novel system to evaluate relative DNA-binding affinities of chemical compounds with primer-dimer(PD) templates using a real-time PCR analyzer. The utility of PD templates allows us to be accurate and highly sensitive determination of DNA-binding affinities of chemical compounds without the amplification of non-specific PCR products. We also developed the colorimetric determination system of DNA-binding affinities of chemical compounds in combination with gold nanoparticle aggregation phenomena and PCR-based evaluation system described above.
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Report
(4 results)
Research Products
(8 results)
