Exploration of biosurfactant to develop high efficiency and safety vector for gene transfection
| Project/Area Number |
21790047
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
INOH Yoshikazu Aichi Gakuin University, 薬学部, 講師 (90434547)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 遺伝子治療 / 遺伝子導入 / 非ウイルスベクター / 正電荷リポソーム / バイオサーファクタントクタント / バイオサーファクタント / 不飽和度 |
|---|
| Research Abstract |
An unsaturated hydrocarbon chain in phospholipid was reported to affect a phase transition and a fusogenic activity after mixing membranes, and consequently to achieve a high DNA transfection efficiency. We previously showed that a biosurfactant mannosylerythritol lipid-A (MEL-A) enhances the gene transfection efficiency of cationic liposomes. Here, we have studied the effects of unsaturated fatty acid ratio of MEL-A on the gene transfer efficiency by cationic liposomes using MEL-A with different unsaturated fatty acid ratios. As a result, we demonstrated that the MEL-A unsaturated fatty acid ratio strongly affects transfection efficiency.
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Report
(3 results)
Research Products
(18 results)
