Physiological role of PAFAH2, an oxidized phospholipid-hydrolyzing enzyme and PAFAH2-derived bioactive lipids
Project/Area Number |
21790060
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Biological pharmacy
|
Research Institution | The University of Tokyo |
Principal Investigator |
KONO Nozomu The University of Tokyo, 大学院・薬学系研究科, 助教 (50451852)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | PAF-AH / 酸化リン脂質 / 酸化脂肪酸 / イソプロスタン |
Research Abstract |
In this study, we obtained following results: (1) Using immunoprecipitation coupled with mass spectrometry, several PAFAH2-interacting proteins including PRDX2 and SCP2 were identified. (2) From mutational analysis of PAFAH2, Cys-83 was found to be the only residue conferring sensitivity of PAFAH2 to DTNB, suggesting that PAFAH2 activity can be regulated by modification of Cys-83. (3) An immunocytochemical assay to detect the translocation of PAFAH2 from cytosol to membrane was established. (4) PAFAH2-deficient mice exhibited reduced PCA reaction most probably by impaired mast cell degranulation. (5) Bone marrow-derived mast cells from PAFAH2-deficient mice also showed decreased IgE-dependent degranulation.
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Report
(3 results)
Research Products
(40 results)