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Formation of signaling complex in P2Y12 receptor-mediated microglial chemotaxis.

Research Project

Project/Area Number 21790080
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionKyushu University

Principal Investigator

SAITOH Hidetoshi  Kyushu University, 薬学研究院, 助教 (90444794)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords神経生物学 / ATP受容体 / ミクログリア / 神経科学 / イメージング / 脂質ラフト
Research Abstract

This study established a method which achieved effective transgene expression in primary microglia from neonatal rat forebrain using lentiviral vectors possessing iba1 promoter and transgenes. In addition, this study revealed that two chemokine, CCL2 and CCL3, are upregulated by activation of P2Y12 receptor in primary microglia.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report

Research Products

(29 results)

All 2011 2010 2009 Other

All Journal Article Presentation Book Remarks

  • [Journal Article] Involvement of vasodilator-stimulated phosphoprotein in UDP-induced microglial actin aggregation via PKC- and Rho-dependent pathways2011

    • Author(s)
      Kataoka A, Koga Y, Uesugi A, Tozaki-Saitoh H, Tsuda M, Inoue K
    • Journal Title

      Purinergic Signaling in press

    • Related Report
      2010 Final Research Report
  • [Journal Article] Astrocytic P2Y(1) receptor is involved in the regulation of cytokine/chemokine transcription and cerebral damage in a rat model of cerebral ischemia.2011

    • Author(s)
      Kuboyama K, Harada H, Tozaki-Saitoh H, Tsuda M, Ushijima K, Inoue K.
    • Journal Title

      J Cereb Blood Flow Metab. in press

    • Related Report
      2010 Final Research Report
  • [Journal Article] Neuronal CCL21 up-regulates microglia P2X4 expression and initiates neuropathic pain development.2011

    • Author(s)
      Biber K, Tsuda M, Tozaki-Saitoh H, Tsukamoto K, Toyomitsu E, Masuda T, Boddeke H, Inoue K.
    • Journal Title

      EMBO J 30(9)

      Pages: 73-1864

    • Related Report
      2010 Final Research Report
  • [Journal Article] JAK-STAT3 pathway regulates spinal astrocyte proliferation and neuropathic pain maintenance in rats.2011

    • Author(s)
      Tsuda M, Kohro Y, Yano T, Tsujikawa T, Kitano J, Tozaki-Saitoh H, Koyanagi S, Ohdo S, Ji R-R, Salter MW, Inoue K
    • Journal Title

      Brain 134

      Pages: 39-1127

    • Related Report
      2010 Final Research Report
  • [Journal Article] JAK-STAT3 pathway regulates spinal astrocyte proliferation and neuropathic pain maintenance in rats.2011

    • Author(s)
      Tsuda M, (他10名)
    • Journal Title

      Brain

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuronal CCL21 up-regulates microglia P2X4 expression and initiates neuropathic pain development.2011

    • Author(s)
      Biber K, (他7名)
    • Journal Title

      EMBO Journal

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purinergic regulation of microglia2010

    • Author(s)
      Tozaki-Saitoh H, Tsuda M, Inoue K
    • Journal Title

      日本薬理学雑誌 136(2)

      Pages: 7-93

    • NAID

      10026631861

    • Related Report
      2010 Final Research Report
  • [Journal Article] Nerve injury-activated microglia engulf myelinated axons in a P2Y12 signaling-dependent manner in the dorsal horn.2010

    • Author(s)
      Maeda M, Tsuda M, Tozaki-Saitoh H, Inoue K, Kiyama H
    • Journal Title

      Glia. 58(15)

      Pages: 46-1838

    • Related Report
      2010 Final Research Report
  • [Journal Article] Pain and purinergic signaling.2010

    • Author(s)
      Tsuda M, Tozaki-Saitoh H, Inoue K
    • Journal Title

      Brain Res Rev. 63(1-2)

      Pages: 32-222

    • Related Report
      2010 Final Research Report
  • [Journal Article] P2X7 receptor activation induces CXCL2 production in microglia through NFAT and PKC/MAPK pathways.2010

    • Author(s)
      Shiratori M, Tozaki-Saitoh H, Yoshitake M, Tsuda M, Inoue K
    • Journal Title

      J Neurochem. 114(3)

      Pages: 9-810

    • Related Report
      2010 Final Research Report
  • [Journal Article] P2X7 receptor activation induces CXCL2 production in microglia through NFAT and PKC/MAPK pathways.2010

    • Author(s)
      Shiratori M, (他4名)
    • Journal Title

      Journal of Neurochemistry

      Volume: 114 Pages: 810-819

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pain and purinergic signaling.2010

    • Author(s)
      Tsuda M, (他2名)
    • Journal Title

      Brain research reviews

      Volume: 63 Pages: 222-232

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nerve injury-activated microglia engulf myelinated axons in a P2Y12 signaling-dependent manner in the dorsal horn.2010

    • Author(s)
      Maeda M, (他4名)
    • Journal Title

      Glia

      Volume: 58 Pages: 1838-1846

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purinergic regulation of microglia2010

    • Author(s)
      齊藤秀俊, (他2名)
    • Journal Title

      日本薬理学雑誌

      Volume: 136 Pages: 93-97

    • NAID

      10026631861

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Behavioral phenotypes of mice lacking purinergic P2X4 receptors in acute and chronic pain assays.2009

    • Author(s)
      Tsuda M, Kuboyama K, Inoue T, Nagata K, Tozaki-Saitoh H, Inoue K
    • Journal Title

      Mol.Pain;11 5(1)

      Pages: 28-28

    • Related Report
      2010 Final Research Report
  • [Journal Article] Intrathecal delivery of PDGF produces tactile allodynia through its receptors in spinal microglia2009

    • Author(s)
      Masuda J, Tsuda M, Tozaki-Saitoh H, Inoue K
    • Journal Title

      Molecular Pain 5

      Pages: 23-23

    • Related Report
      2010 Final Research Report
  • [Journal Article] Antidepressants inhibit P2X4 receptor function: a possible involvement in neuropathic pain relief.2009

    • Author(s)
      Nagata K, Imai K, Yamashita T, Tozaki-Saitoh H, Tsuda M, Inoue K
    • Journal Title

      Mol.Pain,23. 5

      Pages: 20-20

    • Related Report
      2010 Final Research Report
  • [Journal Article] IFN-{gamma} receptor signaling mediates spinal microglia activation driving neuropathic pain.2009

    • Author(s)
      Tsuda M, Masuda T, Kitano J, Shimoyama H, Tozaki-Saitoh H, Inoue K
    • Journal Title

      Proc Natl Acad Sci U S A. 106(19)

      Pages: 7-8032

    • Related Report
      2010 Final Research Report
  • [Journal Article] Mechanisms underlying fibronectin-induced upregulation of P2XR expression in microglia: distinct roles of PI3K-Akt and MEK-ERK signaling pathways.2009

    • Author(s)
      Tsuda M, Toyomitsu E, Kometani M, Tozaki-Saitoh H, Inoue K
    • Journal Title

      J Cell Mol Med. 13(9B)

      Pages: 9-3251

    • Related Report
      2010 Final Research Report
  • [Journal Article] P2Y(1) receptor signaling enhances neuroprotection by astrocytes against oxidative stress via IL-6 release in hippocampal cultures.2009

    • Author(s)
      Fujita T, Tozaki-Saitoh H, Inoue K
    • Journal Title

      Glia 57(3)

      Pages: 57-244

    • Related Report
      2010 Final Research Report
  • [Presentation] P2Y12 receptors in spinal microglia are required for neuropathic pain after peripheral nerve injury2011

    • Author(s)
      齊藤秀俊, (他4名)
    • Organizer
      第84回日本薬理学会
    • Place of Presentation
      (誌上開催)
    • Year and Date
      2011-03-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] ミクログリアの貪食時における-過性のカルシウム上昇2010

    • Author(s)
      齋藤秀俊
    • Organizer
      第83回日本薬理学会年会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2010-03-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 神経障害性疼痛発症時の脊髄CCL3の関与2010

    • Author(s)
      齊藤秀俊、上田和明、津田誠、井上和秀
    • Organizer
      第83回日本薬理学会年会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] ミクログリアの貪食時における一過性のカルシウム上昇2010

    • Author(s)
      齊藤秀俊、津田誠、井上和秀
    • Organizer
      第83回日本薬理学会年会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] P2Y12 receptor in spinal microglia are required for neuropathic pain2009

    • Author(s)
      Tozaki-Saitoh Hidetoshi
    • Organizer
      International symposium on Purinergic signalling in New strategy of drug discovery,Joint with JSPS Core-to-Core Program,A satellite symposium for IUPS 2009
    • Place of Presentation
      Fukuoka
    • Related Report
      2010 Final Research Report
  • [Book] Int Rev Neurobiol 852009

    • Author(s)
      Inoue K, Koizumi S, Kataoka A, Tozaki-Saitoh H, Tsuda M
    • Publisher
      Chapter 12 P2Y(6)-Evoked Microglial Phagocytosis.
    • Related Report
      2010 Final Research Report
  • [Book] 脊髄ミクログリアのP2プリン受容体と神経障害性疼痛、生体の科学「伝達物質と受容体」特集号602009

    • Author(s)
      井上和秀、津田誠、齊藤秀俊
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://yakkou.phar.kyushu-u.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://yakkou.phar.kyushu-u.ac.jp/Publication.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-03-31   Modified: 2016-04-21  

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