Molecular assembly in functional Ca^<2+> microdomain in vascular smooth muscle cells

• Project/Area Number: 21790087
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: YAMAMURA Hisao Nagoya City University, 大学院・薬学研究科, 講師 (80398362)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: イオンチャネル / カルシウムシグナル / 平滑筋 / 全反射蛍光顕微鏡 / 高血圧 / カリウムチャネル / カルシウムチャネル / リアノジン受容体 / カベオリン / マイクロドメイン / 一分子可視化 / 血管平滑筋

Research Abstract

Calcium sparks occur as spontaneous calcium release in subplasmalemmal regions under physiological and pathological conditions in smooth muscle cells. Spatiotemporal imaging of molecular assembly and calcium dynamics in functional calcium microdomain using a total internal reflection fluorescence microscope enables us to elucidate its physiological impact in the regulation of membrane excitability and myogenic tone in vascular smooth muscle cells.

Research Products

• [Journal Article] Contribution of Kir2 potassium channels to ATP-induced cell death in brain capillary endothelial cells and reconstituted HEK293 cell model. (2011)
  • Author(s): Yamazaki D, Kito H, Yamamoto S, Ohya S, Yamamura H, Asai K, Imaizumi Y.
  • Journal Title: Am J Physiol Cell Physiol 300
  • Peer Reviewed
• [Journal Article] Contribution of Kir2 potassium channels to ATP-induced cell death in brain capillary endothelial cells and reconstituted HEK293 cell model (2011)
  • Author(s): Yamazaki D, Kito H, Yamamoto S, Ohya S, Yamamura H, Asai K, Imaizumi Y
  • Journal Title: Am J Physiol Cell Physiol Volume: 300
  • Peer Reviewed
• [Journal Article] Novel spliced variants of large-conductance Ca^<2+>-activated K^+-channel β_2-subunit in human and rodent pancreas. (2010)
  • Author(s): Ohya S, Fujimori T, Kimura T, Yamamura H, Imaizumi Y.
  • Journal Title: J Pharmacol Sci 114 Pages: 198-205
  • NAID: 10027746682
  • Peer Reviewed
• [Journal Article] Contribution of chloride channel conductance to the regulation of resting membrane potential in chondrocytes. (2010)
  • Author(s): Funabashi K, Fujii M, Yamamura H, Ohya S, Imaizumi Y.
  • Journal Title: J Pharmacol Sci 113 Pages: 94-99
  • NAID: 10027745243
  • Peer Reviewed
• [Journal Article] Accelerated Ca^<2+> entry by membrane hyperpolarization due to Ca^<2+>-activated K^+ channel activation in response to histamine in chondrocytes. (2010)
  • Author(s): Funabashi K, Ohya S, Yamamura H, Hatano N, Muraki K, Giles W, Imaizumi Y.
  • Journal Title: Am J Physiol Cell Physiol 298
  • Peer Reviewed
• [Journal Article] Cellular Ca^<2+> dynamics in urinary bladder smooth muscle from transgenic mice overexpressing Na^+-Ca^<2+> exchanger. (2010)
  • Author(s): Murata H, Hotta S, Sawada E, Yamamura H, Ohya S, Kita S, Iwamoto T, Imaizumi Y.
  • Journal Title: J Pharmacol Sci 112 Pages: 373-377
  • NAID: 10027744717
  • Peer Reviewed
• [Journal Article] Accelerated Ca^<2+> entry by membrane hyperpolarization due to Ca^<2+>-activated K^+ channel activation in response to histamine in chondrocytes (2010)
  • Author(s): Funabashi K, Ohya S, Yamamura H, Hatano N, Muraki K, Giles W, Imaizumi Y
  • Journal Title: Am J Physiol Cell Physiol Volume: 298
  • Peer Reviewed
• [Journal Article] Contribution of chloride channel conductance to the regulation of resting membranepotential in chondrocytes (2010)
  • Author(s): Funabashi K, Fujii M, Yamamura H, Ohya S, Imaizumi Y
  • Journal Title: J Pharmacol Sci Volume: 113 Pages: 94-9
  • Peer Reviewed
• [Journal Article] Novel spliced variants of large-conductance Ca^<2+>-activated K^+-channel β_2-subunit in human and rodent pancreas (2010)
  • Author(s): Ohya S, Fujimori T, Kimura T, Yamamura H, Imaizumi Y
  • Journal Title: J Pharmacol Sci Volume: 114 Pages: 198-205
  • NAID: 10027746682
  • Peer Reviewed
• [Journal Article] Accelerated Ca^<2+> entry by membrane hyperpolarization due to Ca^<2+>-activated K^+ channel activation in response to histamine in chondrocytes (2010)
  • Author(s): Funabashi K, Ohya S, Yamamura H, Hatano N, Muraki K, Giles W, Imaizumi Y
  • Journal Title: Am J Physiol Cell Physiol 298(4)
  • Peer Reviewed
• [Journal Article] Cellular Ca^<2+> dynamics in urinary bladder smooth muscle from transgenic miceoverexpressing Na^+-Ca^<2+> exchanger (2010)
  • Author(s): Murata H, Hotta S, Sawada E, Yamamura H, Ohya S, Kita S, Iwamoto T, Imaizumi Y
  • Journal Title: J Pharmacol Sci 112(3) Pages: 373-7
  • NAID: 10027744717
  • Peer Reviewed
• [Journal Article] Inhibition of Kv1.3 potassium current by phosphoinositides and stromal-derived factor-1α in Jurkat T cells. (2009)
  • Author(s): Matsushita Y, Ohya S, Suzuki Y, Itoda H, Kimura T, Yamamura H, Imaizumi Y.
  • Journal Title: Am J Physiol Cell Physiol 296
  • Peer Reviewed
• [Journal Article] Regulation of ryanodine receptor-mediated Ca^<2+> release in vas deferens smooth muscle cells. (2009)
  • Author(s): Ohno A, Ohya S, Yamamura H, Imaizumi Y.
  • Journal Title: J Pharmacol Sci 110 Pages: 78-86
  • NAID: 10025736704
  • Peer Reviewed
• [Journal Article] Regulation of ryanodine receptor-mediated Ca^<2+> release in vas deferens smooth muscle cells (2009)
  • Author(s): Ohno A, Ohya S, Yamamura H, Imaizumi Y
  • Journal Title: J Pharmacol Sci 110(1) Pages: 78-86
  • NAID: 10025736704
  • Peer Reviewed
• [Journal Article] Inhibition of Kv1.3 potassium current by phosphoinositides and stromal-derived factor-1α in Jurkat T cells (2009)
  • Author(s): Matsushita Y, Ohya S, Suzuki Y, Itoda H, Kimura T, Yamamura H, Imaizumi Y.
  • Journal Title: Am J Physiol Cell Physiol 296(5) Pages: 1079-85
  • Peer Reviewed
• [Journal Article] Involvement of dominant-negative, spliced variants of the intermediate-conductance Ca^<2+>-activated K^+ channel, K_<Ca>3.1 in immune function of lymphoid cells.
  • Author(s): Ohya S, Niwa S, Yanagi A, Fukuyo Y, Yamamura H, Imaizumi Y.
  • Journal Title: J Biol Chem in press.
  • Peer Reviewed
• [Journal Article] Involvement of dominant-negative, spliced variants of the intermediate-conductance Ca^<2+>-activated K^+ channel, K_<ca>3.1 in immune function of lymphoid cells
  • Author(s): Ohya S, Niwa S, Yanagi A, Fukuyo Y, Yamamura H, Imaizumi Y
  • Journal Title: J Biol Chem Volume: (in press)
  • Peer Reviewed
• [Journal Article] Contribution of chloride channel conductance to the regulation of resting membrane potential in chondrocytes
  • Author(s): Funabashi K, Fujii M, Yamamura H, Ohya S, Imaizumi Y
  • Journal Title: J Pharmacol Sci (in press)
  • NAID: 10027745243
  • Peer Reviewed
• [Presentation] Molecular assembly in functional Ca^<2+> microdomain in vascular smooth muscle cells. (2010)
  • Author(s): Yamamura H, Ohya S, Imaizumi Y.
  • Organizer: 16th World Congress on Basic and Clinical Pharmacology (WorldPharm 2010).
  • Place of Presentation: Copenhagen, DENMARK.
  • Year and Date: 2010-07-22
• [Presentation] Molecular assembly in functional Ca^<2+> microdomain in vascular smooth muscle cells (2010)
  • Author(s): Yamamura H, Ohya S, Imaizumi Y
  • Organizer: 16th World Congress on Basic and Clinical Pharmacology (WorldPharm 2010)
  • Place of Presentation: Copenhagen, DENMARK
  • Year and Date: 2010-07-22
• [Presentation] 血管平滑筋細胞のCa^<2+>マイクロドメインを構成する分子集積群の可視化解析 (2010)
  • Author(s): 山村寿男, 大矢進, 今泉祐治
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] 血管平滑筋細胞のCa^<2+>マイクロドメインを構成する分子集積群の可視化解析 (2010)
  • Author(s): 山村寿男、大矢進、今泉祐治
  • Organizer: 日本薬学会 第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] 血管平滑筋Ca^<2+>スパークドメインを構成する分子集積群の可視化解析 (2010)
  • Author(s): 山村寿男, 大矢進, 今泉祐治
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-16
• [Presentation] 血管平滑筋Ca^<2+>スパークドメインを構成する分子集積群の可視化解析 (2010)
  • Author(s): 山村寿男、大矢進、今泉祐治
  • Organizer: 第83回 日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-16
• [Presentation] 血管平滑筋カルシウムマイクロドメインを構成する分子群の一分子可視化解析 (2009)
  • Author(s): 山村寿男, 大矢進, 今泉祐治
  • Organizer: 生体機能と創薬シンポジウム2009【招待講演】
  • Place of Presentation: 東京
  • Year and Date: 2009-08-26
• [Presentation] 血管平滑筋カルシウムマイクロドメインを構成する分子群の-分子可視化解析 (2009)
  • Author(s): 山村寿男、大矢進、今泉祐治
  • Organizer: 生体機能と創薬シンポジウム2009
  • Place of Presentation: 東京
  • Year and Date: 2009-08-26
• [Presentation] TIRF imaging of Ca^<2+> spark microdomain in vascular smooth muscle cells. (2009)
  • Author(s): Yamamura H, Ohya S, Imaizumi Y.
  • Organizer: The 36th Congress of the International Union of Physiological Sciences (IUPS 2009).
  • Place of Presentation: Kyoto, JAPAN.
  • Year and Date: 2009-07-29
• [Presentation] TIRF imaging of Ca^<2+> spark microdomain in vascular smooth muscle cells (2009)
  • Author(s): Yamamura H, Ohya S, Imaizumi Y
  • Organizer: The 36th Congress of the International Union of Physiological Sciences (IUPS 2009)
  • Place of Presentation: Kyoto, JAPAN
  • Year and Date: 2009-07-29
• [Presentation] Molecular assembly in Ca^<2+> microdomain of spark sites in vascular smooth muscle cells. (2009)
  • Author(s): Yamamura H, Ohya S, Imaizumi Y.
  • Organizer: Satellite Symposium of the IUPS2009 "Post-genomic Advances in the Physiology of Smooth Muscle" (ISSM2009).
  • Place of Presentation: Nagoya, JAPAN.
  • Year and Date: 2009-07-23
• [Presentation] Molecular assembly in Ca^<2+> microdomain of spark sites in vascular smooth muscle cells (2009)
  • Author(s): Yamamura H, Ohya S, Imaizumi Y
  • Organizer: Satellite Symposium of the IUPS2009 "Post-genomic Advances in the Physiology of Smooth Muscle" (ISSM2009)
  • Place of Presentation: Nagoya, JAPAN
  • Year and Date: 2009-07-23
• [Presentation] 血管平滑筋Ca^<2+>スパークドメインに集積する分子群の一分子可視化解析 (2009)
  • Author(s): 山村寿男, 大矢進, 今泉祐治
  • Organizer: 特定領域研究「生体膜トランスポートソームの分子構築と生理機能」平成21年度第1回班会議
  • Place of Presentation: 熊本
• [Presentation] 血管平滑筋Ca^<2+>スパークドメインに集積する分子群の-分子可視化解析 (2009)
  • Author(s): 山村寿男、大矢進、今泉祐治
  • Organizer: 特定領域研究「生体膜トランスポートソームの分子構築と生理機能」平成21年度第1回班会議
  • Place of Presentation: 熊本
• [Remarks] ホームページ情報
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  • URL: http://www.phar.nagoya-cu.ac.jp/hp/ysg/index.html
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  • URL: http://www.phar.nagoya-cu.ac.jp/hp/ysg/index.html
• [Patent(Industrial Property Rights)] イオンチャネルに作用する化合物のスクリーニング用材料及びその利用 (2010)
  • Inventor(s): 今泉祐治, 藤井将人, 大矢進, 山村寿男
  • Industrial Property Rights Holder: 公立大学法人名古屋市立大学,有限会社チャネロサーチテクノロジー
  • Filing Date: 2010-06-28
• [Patent(Industrial Property Rights)] イオンチャネルに作用する化合物のスクリーニング用材料及びその利用 (2010)
  • Inventor(s): 今泉祐治、藤井将人、大矢進、山村寿男
  • Industrial Property Rights Holder: 公立大学法人名古屋市立大学、有限会社チャネロサーチテクノロジー
  • Filing Date: 2010-06-28