Physiological roles of a novel cannabinoid receptor GPR55 and its endogenous ligand

• Project/Area Number: 21790095
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Teikyo University
• Principal Investigator: OKA Saori Teikyo University, 薬学部, 講師 (80439562)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 生化学 / 脂質生化学 / カンナビノイド / オーファン受容体 / リゾリン脂質 / GPR55 / 生理活性脂質 / リゾホスファチジルイノシトール / GPR35 / リゾホスファチジン酸

Research Abstract

GPR55 is a putative novel cannabinoid receptor and its endogenous ligand is lysophosphatidylinositol (LPI). This study revealed that LPI plays some essential roles in the regulation of cytoskeletal functions and stress responses. Furthermore, we searched for the ligand for GPR35 which has 30% homology with GPR55, and found that 2-acyl lysophosphatidic acid (LPA) may act as an endogenous ligand for GPR35.

Research Products

• [Journal Article] Lysophosphatidylinositol induces rapid phosphorylation of p38 mitogen-activated protein kinase and activating transcription factor 2 in HEK293cells expressing GPR55 and IM-9 lymphoblastoid cells. (2010)
  • Author(s): Oka, S., Kimura S, Sugiua, T., et al.
  • Journal Title: J.Biochem. 147 Pages: 671-678
  • Peer Reviewed
• [Journal Article] GPR35 is a novel lysophosphatidic acid receptor. (2010)
  • Author(s): Oka, S., Ota, R., Sugiura, T., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 395 Pages: 232-237
  • Peer Reviewed
• [Journal Article] Generation of lysophosphatidylinositol by DDHD domain containing 1 (DDHD1) : Possible involvement of phospholipase D/phosphatidic acid in the activation of DDHD1. (2010)
  • Author(s): Yamashita, A., Oka, S., Sugiura, T., 他3人
  • Journal Title: Biochem.Biophys.Acta 1801 Pages: 711-720
  • Peer Reviewed
• [Journal Article] Lysophosphatidylinositol induces rapid phosphorylation of p38 mitogen-activated protein kinase and activating transcription factor 2 in HEK293 cells expressing GPR55 and IM-9 lymphoblastoid cells (2010)
  • Author(s): Saori Oka, et al.
  • Journal Title: J.Biochem. Volume: 147 Pages: 671-678
  • NAID: 10027875740
  • Peer Reviewed
• [Journal Article] GPR35 is a novel lysophosphatidic acid receptor (2010)
  • Author(s): Saori Oka, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 395 Pages: 232-237
  • Peer Reviewed
• [Journal Article] Generation of lysophospbatidylinositol by DDHD domain containing 1(DDHD1) : Possible involvement of phospholipase D/phosphatidic acid in the activation of DDHD1 (2010)
  • Author(s): Atsushi Yamashita, et al.
  • Journal Title: Biochem.Biophys.Acta Volume: 1801 Pages: 711-720
  • Peer Reviewed
• [Presentation] Δ^9-テトラヒドロカンナビノール受容体としてのGPR35 (2010)
  • Author(s): 岡沙織, 他
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] BIOLOGICAL ACTIVITIES OF LYSOHOSPHATIDYLINOSITOL AND SEVERAL CANNABINOID RECEPTOR LIGANDS AND RELATED MOLECULES AS GPR55 LIGANDS (2010)
  • Author(s): 岡沙織, 他
  • Organizer: 第26回国際カンナビノイド学会(ICRS2010)
  • Place of Presentation: Scandic star(Sweden)
  • Year and Date: 2010-07-26
• [Presentation] BIOLOGICAL ACTIVITIES OF LYSOHOSPHATIDYLINOSITOL AND SEVERAL CANNABINOID RECEPTOR LIGANDS AND RELATED MOLECULES AS GPR55 LIGANDS (2010)
  • Author(s): 岡沙織, 他
  • Organizer: 第26回 国際カンナビノイド学会(ICRS2010)
  • Place of Presentation: Scandic star (Sweden)
  • Year and Date: 2010-07-26
• [Presentation] Novel lysophospholipid receptors : identification and signaling pathways (2010)
  • Author(s): 岡沙織, 他
  • Organizer: 第27回内藤コンファレンス
  • Place of Presentation: シャトレーゼガトーキングダム札幌
  • Year and Date: 2010-06-30
• [Presentation] Novel lysophospholipid receptors : identification and signaling pathways (2010)
  • Author(s): 岡沙織, 他
  • Organizer: 第27回 内藤コンファレンス
  • Place of Presentation: シャトレーゼガトーキングダム札幌
  • Year and Date: 2010-06-30
• [Presentation] Gタンパク質共役型受容体GPR35の内在性リガンドの同定 (2010)
  • Author(s): 岡沙織, 他
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 伊香保温泉森秋旅館
  • Year and Date: 2010-06-15
• [Presentation] Gタンパク質共役型受容体GPR35の内在性リガンドの同定 (2010)
  • Author(s): 岡沙織, 他
  • Organizer: 第52回 日本脂質生化学会
  • Place of Presentation: 伊香保温泉森秋旅館
  • Year and Date: 2010-06-15
• [Presentation] 新規リゾリン脂質受容体の探索 (2009)
  • Author(s): 岡沙織, 他
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] GPR55発現細胞のリゾホスファチジルイノシトール(LPI)によるRhoを介した活性化 (2009)
  • Author(s): 岡沙織, 他
  • Organizer: 第51回日本脂質生化学会
  • Place of Presentation: ウィルあいち
  • Year and Date: 2009-07-31
• [Presentation] GPR55発現細胞のリゾホスファチジルイノシトール(LPI)によるRhoを介した活性化 (2009)
  • Author(s): 岡沙織, 他
  • Organizer: 第51回日本脂質生化学会
  • Place of Presentation: ウィルあいち(名古屋)
  • Year and Date: 2009-07-31
• [Presentation] LYSOPHOSPHATIDYLINOSITOL INDUCES RAPID PHOSPHORYLATION OFP38 MITOGEN-ACTIVATING PROTEIN KINASE IN HEK293 CELLS EXPRESSING GPR55 (2009)
  • Author(s): 岡沙織, 他
  • Organizer: 国際カンナビノイド会議(ICRS)
  • Place of Presentation: ST.CHARLES, (ILLINOIS, USA)
  • Year and Date: 2009-07-08
• [Presentation] LYSOPHOSPHATIDYLINOSITOL INDUCES RAPID PHOSPHORYLATION OF P38 MITOGEN-ACTIVATING PROTE-N KINASE IN HEK293 CELLS EXPRESSING GPR55 (2009)
  • Author(s): 岡沙織, 他
  • Organizer: 国際カンナビノイド会議(ICRS)
  • Place of Presentation: ST. CHARLES, ILLINOIS USA
  • Year and Date: 2009-07-08
• [Presentation] Gタンパク質共役型受容体GPR55発現細胞のリゾホスファチジルイノシトール(LPI)によるRhoAを介した活性化 (2009)
  • Author(s): 岡沙織, 他
  • Organizer: 第10回Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 日本薬学会長井記念館
  • Year and Date: 2009-06-20
• [Presentation] EFECTS OF 2-ARACHIDONOYL LYSOPHOSPHATIDYLINOSITOL ON HEK293 CELLS EXPRESSING GPR55 (2009)
  • Author(s): 岡沙織, 他
  • Organizer: 4th International Conference on Phospholipase A2 and Lipid Mediators(PLM2009)
  • Place of Presentation: 学術総合センター
  • Year and Date: 2009-05-27
• [Presentation] EFECTS OF 2-ARACHIDONOYL LYSOPHOSPHATIDYLINOSITOL ON HEK293 CELLS EXPRESSING GPR55 (2009)
  • Author(s): 岡沙織, 他
  • Organizer: 4th International Conference on Phospholipase A2 and Lipid Mediators (PLM2009)
  • Place of Presentation: 学術総合センター(竹橋)
  • Year and Date: 2009-05-27
• [Remarks]
  • URL: http://www.pharm.teikyo-u.ac.jp/lab/eisei/index.html
• [Remarks]
  • URL: http://www.pharm.teikyo-u.ac.jp/lab/eisei/index.html