Mechanism underlying induction of human CYP1A1 gene by major phytocannabinoids

• Project/Area Number: 21790135
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: Hokuriku University
• Principal Investigator: YAMAORI Satoshi Hokuriku University, 薬学部, 助教 (40360218)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 中毒学 / 異物代謝 / 分子毒性学 / 大麻 / フィトカンナビノイド / カンナビジオール / ヒトCYP1A1 / HeG2細胞 / AhR / PTK / A549細胞 / 非幻覚成分 / HepG2細胞 / 発現誘導

Research Abstract

Effects of marijuana component(s), especially phytocannabinoids, on expression of cytochrome P450 1A1 (CYP1A1) in human hepatic cells were examined to clarify whether or not marijuana component(s) induces the expression of CYP1A1. Cannabidiol, one of the major phytocannabinoids in marijuana, increased the expression level of hepatic CYP1A1. The induction of CYP1A1 by cannabidiol may be mediated through common and/or uncommon pathway(s).

Research Products

• [Journal Article] Potent inhibition of human cytochrome P450 3A isoforms by cannabidiol : role of phenolic hydroxyl groups in the resorcinol moiety. (2011)
  • Author(s): Satoshi Yamaori, Juri Ebisawa, Yoshimi Okushima, Ikuo Yamamoto, Kazuhito Watanabe
  • Journal Title: Life Sci. Vol.88 Pages: 730-736
  • Peer Reviewed
• [Journal Article] Potent inhibition of human cytochrome P450 3A isoforms by cannabidiol : role of phenolic hydroxyl groups in the resorcinol moiety (2011)
  • Author(s): S.Yamaori, J.Ebisawa, Y.Okushima, I.Yamamoto, K.Watanabe
  • Journal Title: Life Sci. Volume: 88 Pages: 730-736
  • Peer Reviewed
• [Journal Article] Characterization of major phytocannabinoids, cannabidiol and cannabinol, as isoform-selective and potent inhibitors of human CYP1 enzymes. (2010)
  • Author(s): Satoshi Yamaori, Mika Kushihara, Ikuo Yamamoto, Kazuhito Watanabe
  • Journal Title: Biochem.Pharmacol. Vol.79 Pages: 1691-1698
  • Peer Reviewed
• [Journal Article] Characterization of major phytocannabinoids, cannabidiol and cannabinol, as isoform-selective and potent inhibitors of human CYP1 enzymes (2010)
  • Author(s): S.Yamaori, M.Kushihara, I.Yamamoto, K.Watanabe
  • Journal Title: Biochem.Pharmacol. Volume: 79 Pages: 1691-1698
  • Peer Reviewed
• [Presentation] 大麻非幻覚成分カンナビジオールによるヒトCYPIA1発現誘導 (2009)
  • Author(s): 山折大
  • Organizer: フォーラム2009衛生薬学・環境トキシコロジー
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2009-11-05
• [Presentation] 大麻非幻覚成分カンナビジオールによるヒトCYP1A1発現誘導 (2009)
  • Author(s): 山折大
  • Organizer: フォーラム2009 衛生薬学・環境トキシコロジー
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2009-11-05