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Mechanisms of heavy metal-dependent transactivation by transcription factor MTF-1

Research Project

Project/Area Number 21790137
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Environmental pharmacy
Research InstitutionSetsunan University

Principal Investigator

KIMURA Tomoki  Setsunan University, 薬学部, 講師 (70340859)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords亜鉛 / MTF-1 / メタロチオネイン / カドミウム
Research Abstract

Zinc is an essential micronutrient. It is critically important to control intracellular zinc concentrations tightly. In mammals, metallothionein (MT), a small metal-binding protein, plays important roles in zinc homeostasis. Mouse MT-I gene transcription is regulated by metal response element-binding transcription factor-1 (MTF-1), which is recruited to the promoter by zinc. We examined alterations in the chromatin structure of the MT-I promoter associated with enhanced transcriptional activation. Here, chromatin immunoprecipitation assays and micrococcal nuclease sensitivity of the MT-I promoter demonstrated that the chromatin structure in the promoter may be locally disrupted by zinc-induced nucleosome removal. Rapid disruption of nucleosome structure at the MT-I promoter is mediated by zinc-responsive recruitment of an active MTF-1-coactivator complex.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report

Research Products

(39 results)

All 2011 2010 2009 Other

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (28 results) Remarks (3 results)

  • [Journal Article] Chromium (VI) inhibits mouse metallothionein-I gene transcription by modifying the transcription potential of the co-activator p3002011

    • Author(s)
      木村朋紀、奥村文香、小野寺章、中西剛、伊藤徳夫、磯部正和
    • Journal Title

      The Journal of Toxicological Sciences 36巻

    • NAID

      10028101162

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The zinc-sensing transcription factor MTF-1 mediates zinc-induced epigenetic changes in chromatin of the mouse metallothionein-I promoter2011

    • Author(s)
      奥村文香、Yong Li、伊藤徳夫、中西剛、磯部正和、Glen K.Andrews, 木村朋紀
    • Journal Title

      Biochimica et Biophysica Acta-Gene Regulatory Mechanisms 1809巻

      Pages: 56-62

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The zinc-sensing transcription factor MTF-1 mediates zinc-induced epigenetic changes in chromatin of the mouse metallothionein-1 promoter2011

    • Author(s)
      奥村文香
    • Journal Title

      Biochim Biophys Acta-Gene Regulatory Mechanisms

      Volume: 1809 Pages: 56-62

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chromium (VI) inhibits mouse metallothionein-I gene transcription by modifying the transcription potential of the co-activator p3002011

    • Author(s)
      木村朋紀
    • Journal Title

      Journal of Toxicological Sciences

      Volume: 36(印刷中)

    • NAID

      10028101162

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms of zinc-mediated induction and chromium(VI)-mediated inhibition of mouse metallothionein-I gene transcription2010

    • Author(s)
      木村朋紀
    • Journal Title

      Journal of Health Science 56巻

      Pages: 161-166

    • NAID

      130000254713

    • Related Report
      2010 Final Research Report
  • [Journal Article] Molecular mechanisms of zinc-mediated induction and chromium(VI)-mediated inhibition of mouse metallothionein-I gene transcription2010

    • Author(s)
      木村朋紀
    • Journal Title

      Journal of Health Science 56巻(印刷中)

      Pages: 1-6

    • NAID

      130000254713

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Mechanisms of heavy metal sensing by metal response element-binding transcription factor-12009

    • Author(s)
      木村朋紀、伊藤徳夫、Glen K.Andrews
    • Journal Title

      Journal of Health Science 55巻

      Pages: 484-494

    • NAID

      130000127927

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms of heavy metal sensing by metal response element-binding transcription factor-12009

    • Author(s)
      木村朋紀
    • Journal Title

      Journal of Health Science 55巻

      Pages: 484-494

    • NAID

      130000127927

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 細胞内シグナル伝達系を介した重金属応答性転写因子MTF-1によるメタロチオネイン誘導2010

    • Author(s)
      木村朋紀
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      桃太郎アリーナ(岡山)
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] メタロチオネイン遺伝子の転写に伴うクロマチン内ヒストンH3減少の機序解析2010

    • Author(s)
      古田雄三、木村朋紀、奥村文香、磯部正和
    • Organizer
      第2回メタロミクス研究フォーラム
    • Place of Presentation
      京都薬科大、京都
    • Related Report
      2010 Final Research Report
  • [Presentation] The zinc-sensing transcription factor MTF-1 mediates zinc-induced epigenetic changes in chromatin of the mouse metallothionein-I promoter.2010

    • Author(s)
      木村朋紀、奥村文香、Yong Li、伊藤徳夫、中西剛、磯部正和、Glen K.Andrews
    • Organizer
      第60回藤原セミナー
    • Place of Presentation
      大阪国際会議場、大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] 亜鉛によるメタロチオネイン遺伝子発現に伴うクロマチン構造変化に関与する因子群の解析2010

    • Author(s)
      木村朋紀、古田雄三、奥村文香、磯部正和
    • Organizer
      フォーラム2010:衛生薬学・環境トキシコロジー
    • Place of Presentation
      星薬科大学、東京
    • Related Report
      2010 Final Research Report
  • [Presentation] Chromium (VI) inhibits mouse metallothionein-I gene transcription by modifying transactivation potential of the co-activator p3002010

    • Author(s)
      木村朋紀、奥村文香、小野寺章、中西剛、伊藤徳夫、磯部正和
    • Organizer
      The XII International Congress of Toxicology
    • Place of Presentation
      バルセロナ、スペイン
    • Related Report
      2010 Final Research Report
  • [Presentation] Cr(VI) inhibits mouse metallothionein-I gene transcription by modifying transactivation potential of the co-activator p3002010

    • Author(s)
      木村朋紀、奥村文香、小野寺章、中西剛、伊藤徳夫、磯部正和
    • Organizer
      第20回金属の関与する生体関連反応シンポジウム(SRM2010)
    • Place of Presentation
      徳島文理大、徳島
    • Related Report
      2010 Final Research Report
  • [Presentation] 6価クロムによるメタロチオネイン遺伝子発現抑制機構の解析2010

    • Author(s)
      木村朋紀、奥村文香、小野寺章、中西剛、伊藤 徳夫、磯部正和
    • Organizer
      第37回日本トキシコロジー学会学術年会
    • Place of Presentation
      沖縄コンベンションセンター、沖縄
    • Related Report
      2010 Final Research Report
  • [Presentation] 重金属応答性転写因子MTF-1によるメタロチオネイン誘導における細胞内シグナル伝達系の役割2010

    • Author(s)
      奥村文香、木村朋紀、磯部正和
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      桃太郎アリーナ、岡山
    • Related Report
      2010 Final Research Report
  • [Presentation] 6価クロムによるメタロチオネイン遺伝子発現抑制機構の解析2010

    • Author(s)
      木村朋紀
    • Organizer
      第37回日本トキシコロジー学会学術年会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cr(VI) inhibits mouse metallothionein-I gene transcription by modifying transactivation potential of the co-activator p3002010

    • Author(s)
      木村朋紀
    • Organizer
      第20回金属の関与する生体関連反応シンポジウム(SRM2010)
    • Place of Presentation
      徳島文理大学(徳島)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Chromium(VI) inhibits mouse metallothionein-I gene transcription by modifying transactivation potential of the co-activator p3002010

    • Author(s)
      木村朋紀
    • Organizer
      The XII International Congress of Toxicology
    • Place of Presentation
      バルセロナ(スペイン)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 亜鉛によるメタロチオネイン遺伝子発現に伴うクロマチン構造変化に関与する因子群の解析2010

    • Author(s)
      木村朋紀
    • Organizer
      フォーラム2010:衛生薬学・環境トキシコロジー
    • Place of Presentation
      星薬科大学(東京)
    • Related Report
      2010 Annual Research Report
  • [Presentation] The zinc-sensing transcription factor MTF-1 mediates zinc-induced epigenetic changes in chromatin of the mouse metallothionein-I promoter.2010

    • Author(s)
      木村朋紀
    • Organizer
      第60回 藤原セミナー
    • Place of Presentation
      大阪国際会議場(大阪)
    • Related Report
      2010 Annual Research Report
  • [Presentation] メタロチオネイン遺伝子の転写に伴うクロマチン内ヒストンH3減少の機序解析2010

    • Author(s)
      古田雄三
    • Organizer
      第2回メタロミクス研究フォーラム
    • Place of Presentation
      京都薬科大学(京都)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 重金属応答性転写因子MTF-1による転写活性化機構に関する研究2009

    • Author(s)
      木村朋紀
    • Organizer
      フォーラム2009:衛生薬学・環境トキシコロジー
    • Place of Presentation
      沖縄コンベンションセンター、沖縄
    • Year and Date
      2009-11-06
    • Related Report
      2010 Final Research Report
  • [Presentation] 重金属応答性転写因子MTF-1による転写活性化機構に関する研究2009

    • Author(s)
      木村朋紀
    • Organizer
      フォーラム2009:衛生薬学・環境トキシコロジー
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Year and Date
      2009-11-06
    • Related Report
      2009 Annual Research Report
  • [Presentation] 転写因子MTF-1を介した遺伝子発現にともなうクロマチン構造変化とその持続性2009

    • Author(s)
      奥村文香
    • Organizer
      フォーラム2009:衛生薬学・環境トキシコロジー
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] カドミウムによるメタロチオネイン誘導におけるリン酸化シグナル伝達系の役割2009

    • Author(s)
      木村朋紀
    • Organizer
      メタロチオネインおよびメタルバイオサイエンス研究会2009
    • Place of Presentation
      東京大学山上会館(東京)
    • Year and Date
      2009-10-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Chromium (VI) inhibits mouse metallothionein-I gene transcription by preventing the zinc-dependent formation of an MTF-1.p300 complex2009

    • Author(s)
      木村朋紀
    • Organizer
      The 5th International Congress of Asian Society of Toxicology
    • Place of Presentation
      台北、台湾
    • Year and Date
      2009-09-13
    • Related Report
      2010 Final Research Report
  • [Presentation] Zinc-induced epigenetic changes in the mouse metallothionein-I promoter chromatin are mediated by the zinc-sensing transcription factor MTF-12009

    • Author(s)
      奥村文香、木村朋紀、Yong Li Y、伊藤徳夫、中西剛、曽根知道、磯部正和、Glen K.Andrews
    • Organizer
      The 5th International Congress of Asian Society of Toxicology
    • Place of Presentation
      台北、台湾
    • Year and Date
      2009-09-13
    • Related Report
      2010 Final Research Report
  • [Presentation] Zinc-induced epigenetic changes in the mouse metallothionein-I promoter chromatin are mediated by the zinc-sensing transcription factor MTF-12009

    • Author(s)
      奥村文香
    • Organizer
      The 5th International Congress of Asian Society of Toxicology
    • Place of Presentation
      Taipei Veteran General Hospital(台湾)
    • Year and Date
      2009-09-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] Chromium(VI)inhibits mouse metallothionein-I gene transcription by preventing the zinc-dependent formation of an MTF-1-p300 complex2009

    • Author(s)
      木村朋紀
    • Organizer
      The 5th International Congress of Asian Society of Toxicology
    • Place of Presentation
      Taipei Veterans General Hospital(台湾)
    • Year and Date
      2009-09-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] カドミウムによるメタロチオネイン誘導におけるJNKの役割2009

    • Author(s)
      奥村文香
    • Organizer
      第36回日本トキシコロジー学会学術年会
    • Place of Presentation
      岩手県民情報交流センター(盛岡)
    • Year and Date
      2009-07-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] 転写因子MTF-1を介したマウスメタロチオネイン-I遺伝子発現にともなうクロマチン構造変化2009

    • Author(s)
      木村朋紀
    • Organizer
      第3回日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京)
    • Year and Date
      2009-05-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 転写因子MTF-1を介した遺伝子発現にともなうクロマチン構造変化とその持続性2009

    • Author(s)
      奥村文香、木村朋紀、Yong Li、伊藤徳夫、中西剛、磯部正和、Glen K.Andrews
    • Organizer
      フォーラム2009:衛生薬学・環境トキシコロジー
    • Place of Presentation
      沖縄コンベンションセンター、沖縄
    • Related Report
      2010 Final Research Report
  • [Presentation] カドミウムによるメタロチオネイン誘導におけるリン酸化シグナル伝達系の役割2009

    • Author(s)
      木村朋紀、奥村文香、磯部正和
    • Organizer
      メタロチオネインおよびメタルバイオサイエンス研究会2009
    • Place of Presentation
      東京大学、東京
    • Related Report
      2010 Final Research Report
  • [Presentation] カドミウムによるメタロチオネイン誘導におけるJNKの役割2009

    • Author(s)
      奥村文香、木村朋紀、磯部正和
    • Organizer
      第36回日本トキシコロジー学会学術年会
    • Place of Presentation
      岩手県民情報交流センター、盛岡
    • Related Report
      2010 Final Research Report
  • [Presentation] 転写因子MTF-1を介したマウスメタロチオネイン-I遺伝子発現にともなうクロマチン構造変化2009

    • Author(s)
      木村朋紀、奥村文香、Yong Li、伊藤徳夫、中西剛、曽根知道、磯部正和、Glen K.Andrews
    • Organizer
      第3回日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター、東京
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ

    • URL

      http://www.setsunan.ac.jp/~p-doku/doku-top.htm

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.setsunan.ac.jp/~p-doku/doku-frame-seika.htm

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.setsunan.ac.jp/~p-doku/doku-frame-seika.htm

    • Related Report
      2009 Annual Research Report

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Published: 2009-03-31   Modified: 2016-04-21  

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