Mechanisms of heavy metal-dependent transactivation by transcription factor MTF-1
| Project/Area Number |
21790137
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental pharmacy
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| Research Institution | Setsunan University |
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Principal Investigator |
KIMURA Tomoki Setsunan University, 薬学部, 講師 (70340859)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 亜鉛 / MTF-1 / メタロチオネイン / カドミウム |
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| Research Abstract |
Zinc is an essential micronutrient. It is critically important to control intracellular zinc concentrations tightly. In mammals, metallothionein (MT), a small metal-binding protein, plays important roles in zinc homeostasis. Mouse MT-I gene transcription is regulated by metal response element-binding transcription factor-1 (MTF-1), which is recruited to the promoter by zinc. We examined alterations in the chromatin structure of the MT-I promoter associated with enhanced transcriptional activation. Here, chromatin immunoprecipitation assays and micrococcal nuclease sensitivity of the MT-I promoter demonstrated that the chromatin structure in the promoter may be locally disrupted by zinc-induced nucleosome removal. Rapid disruption of nucleosome structure at the MT-I promoter is mediated by zinc-responsive recruitment of an active MTF-1-coactivator complex.
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Report
(3 results)
Research Products
(39 results)
