Structural diversity of L-type Ca^<2+> channel in sinoatrial node-the possibility of Cav-Cav interaction-
Project/Area Number |
21790199
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
General physiology
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Research Institution | Shiga University of Medical Science |
Principal Investigator |
TOYODA Futoshi Shiga University of Medical Science, 医学部, 助教 (90324574)
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Project Period (FY) |
2009 – 2010
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Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Keywords | 生体膜 / チャネル / トランスポーター / 能動輸送 / 生理学 / 生体分子 / 細胞・組織 / 循環器 / イオンチャネル / ペースメーカー |
Research Abstract |
In the present study, we examined the possibility of the functional assembly of Cav1.2 and Cav1.3 subunits to yield the sustained inward Na^+ current (I_<st>), a pacemaker current in sinoatrial node. Heterologous coexpression of Cav1.2 and Cav1.3 in HEK cells resulted in an ensemble of Ca^<2+> currents attributed to each Cav channel. On the other hand, the chimeric channel consisting of repeat I and II from Cav1.2 (or Cav1.3) and repeat III and IV from Cav1.3 (or Cav1.2) elicited a rapidly inactivating Ca^<2+> current but failed to evoke a Na^+ current similar to I_<st>. Taken together, it is not likely that the heteromeric assembly of Cav1.2 and Cav1.3 subunits underlies I_<st> in sinoatrial node.
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] KCNE2 modulation of Kv4.3 current and its potential role in fatal rhythm disorders.2010
Author(s)
Wu J, Shimizu W, Ding WG, Ohno S, Toyoda F, Itoh H, Zang WJ, Miyamoto Y, Kamakura S, Matsuura H, Nademanee K, Brugada J, Brugada P, Brugada R, Vatta M, Towbin JA, Antzelevitch C, Horie M.
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Journal Title
Heart Rhythm. 7
Pages: 199-205
Related Report
Peer Reviewed
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[Journal Article] D85N, a KCNE1 polymorphism, is a disease-causing gene variant in long QT syndrome.2009
Author(s)
Nishio Y, Makiyama T, Itoh H, Sakaguchi T, Ohno S, Gong YZ, Yamamoto S, Ozawa T, Ding WG, Toyoda F, Kawamura M, Akao M, Matsuura H, Kimura T, Kita T, Horie M.
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Journal Title
J Am Coll Cardiol. 54
Pages: 812-819
Related Report
Peer Reviewed
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[Journal Article] Novel KCNE3 mutation reduces repolarizing potassium current and associatedwith long QT syndrome.2009
Author(s)
Ohno S, Toyoda F, Zankov DP, Yoshida H, Makiyama T, Tsuji K, Honda T, Obayashi K, Ueyama H, Shimizu W, Miyamoto Y, Kamakura S, Matsuura H, Kita T, Horie M.
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Journal Title
Hum Mutat. 30
Pages: 557-563
Related Report
Peer Reviewed
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