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The role of reactive oxygen species in aggravation of liver disease and application to medical care

Research Project

Project/Area Number 21790246
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

MATSUNO Kuniharu  Kyoto Prefectural University of Medicine, 医学研究科, 助教 (50420708)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords薬物治療学 / 慢性肝疾患 / 活性酸素 / NADPH oxidase / 肝疾患
Research Abstract

Hepatic fibrosis is a important step for progression from basic hepatic disease to hepatic cirrhosis and cancer. In this process, hepatic stellate cells (HSCs) were thought to play a central role. Recently it was reported that the expression level of NOX1, a novel catalytic subunit of NADPH oxidase, was increased in HSCs with its activation. The aim of this study was to clarify the role of NOX1 in the development of liver fibrosis using Noxl-deficient mice (NOX1 KO).
After bile duct ligation (BDL), increased expression of NOX1, elevation of ALT and AST levels, progression of hepatic fibrosis and increasing of activated HSC in liver tissue were observed in WT but not NOX1 KO. In primary cultured HSCs, its activation was not affected by NOX1 deficiency. However, cell proliferation was significantly attenuated in HSCs isolated from NOX1 KO. In these cells, significant increase of p27^<Kip1>, a cell cycle suppressor, significant reduction in phosphorylated forms of Akt and FOXO4 were confirmed. In addition, the level of oxidized inactivated form of phosphatase and tensine homolog (PETN), a negative regulator of PI3K/Akt pathway, was significantly decreased in HSCs of NOX1 KO. These results showed that NOX1 derived ROS aggravate hepatic fibrosis by oxidation of PTEN, which followed by Akt/FOXO4/p27^<Kip1> pathway dependent HSC proliferation.

Report

(2 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (8 results)

All 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (2 results)

  • [Journal Article] Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene.2011

    • Author(s)
      Katsuyama M, Hirai H, Iwata K, Ibi M, Matsuno K, Matsumoto M, Yabe-Nishimura C.
    • Journal Title

      FEBS Journal 278

      Pages: 964-972

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential expression of NADPH oxidases in megakaryocytes and their role in polyploidy.2009

    • Author(s)
      McCrann DJ, Eliades A, Makitalo M, Matsuno K, Ravid K.
    • Journal Title

      Blood 114

      Pages: 1243-1249

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] NADPH oxidase isoforms and anti-hypertensive effects of atorvastatin demonstrated in two animal models.2009

    • Author(s)
      Cui W, Matsuno K, Iwata K, Ibi M, Katsuyama M, Kakehi T, Sasaki M, Ikami K, Zhu K, Yabe-Nishimura C.
    • Journal Title

      J Pharmacol Sci 111

      Pages: 260-268

    • NAID

      10026154161

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Receptor activator of nuclear factor-kappaB ligand-induced mouse osteoclast differentiation is associated with switching between NADPH oxidase homologues.2009

    • Author(s)
      Sasaki H, Yamamoto H, Tominaga K, Masuda K, Kawai T, Teshima-Kondo S, Matsuno K, Yabe-Nishimura C, Rokutan K.
    • Journal Title

      Free Radic Biol Med 47

      Pages: 189-199

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nicotinamide adenine dinucleotide phosphate, reduced form (NADPH) oxidase NOX1 promotes proliferation of stellate cells and aggravates liver fibrosis induced by bile duct ligation.

    • Author(s)
      Cui W, Matsuno K, Iwata K, Ibi M, Matsumoto M, Zhang J, Zhu K, Katsuyama M, Torok NJ, Yabe-Nishimura C.
    • Journal Title

      Hepatology in press.

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Physiological Roles of NOX/NADPH Oxidase, the Superoxide-generating Enzyme.

    • Author(s)
      Katsuyama M, Matsuno K, Yabe-Nishimura C.
    • Journal Title

      J Clin Biochem Nutr in press.

    • NAID

      130004466676

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] Noxl-deficiency attenuates liver injury and ensuing fibrosis in bile duct-ligated mice.2010

    • Author(s)
      Wenhao Cui, Kuniharu Matsuno, Chihiro Yabe.
    • Organizer
      第83回日本薬理学会総会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] NOX1-derived Reactive Oxygen Species Promote Cardiomyocyte Apoptosis and Aggravate Cardiac Dysfunction in sepsis.2009

    • Author(s)
      Kuniharu Matsuno, Kazumi Iwata, Wenhao Cui, Chihiro Yabe-Nishimura.
    • Organizer
      The 10th US-Japan-Asia Dialogue on Cardiovascular Diseases and Young Investigator Competition.
    • Place of Presentation
      Tokyo
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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