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NADPH酸化酵素による脂肪分化機構と生活習慣病への応用

Research Project

Project/Area Number 21790258
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionNational Institute of Health Sciences

Principal Investigator

KANDA Yasunari  National Institute of Health Sciences, 薬理部, 室長 (70510387)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsNADPH酸化酵素 / 間葉系幹細胞 / 脂肪分化 / 生活習慣病
Research Abstract

Obesity is characterized by increased adipose tissue mass due to generation of new adipocytes or increase in the size of individual adipocytes. The formation of adipose tissue involves the commitment of mesenchymal stem cells (MSC) to the preadipocyte lineage and the differentiation of preadipocytes into mature adipocytes. To investigate the molecular mechanisms that regulate the differentiation of adipocytes from MSC, we investigated the involvement of reactive oxygen species (ROS) in adipocyte differentiation. We found that the antioxidant N-acetyl-L-cysteine (NAC) blocks adipocyte differentiation. An H2DCF assay revealed that differentiation-inducing agents induced ROS generation. Next, we examined the source of ROS. Knockdown of NAD(P)H oxidase 4 (Nox4) by RNA interference inhibited ROS production and adipocyte differentiation by differentiation-inducing agents. Furthermore, we examined the role of ROS in vivo. Administrating the NAC reduced the amount of fat in mice fed a high fat diet. These results identify ROS as a regulator of the adipogenesis and obesity.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (13 results)

All 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (11 results)

  • [Journal Article] Nicotine increases cancer stem cell population in MCF-7 cells.2010

    • Author(s)
      Naoya Hirata, Yuko Sekino, Yasunari Kanda.
    • Journal Title

      Biochemical and Biophysical Research Communications 403

      Pages: 138-143

    • Related Report
      2010 Final Research Report
  • [Journal Article] Nicotine increases cancer stem cell population in MCF-7 cells2010

    • Author(s)
      Hirata N, Sekino Y, Kanda Y.
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 403 Pages: 138-143

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] MCF-7細胞における乳癌幹細胞に対するエストロゲンの影響2011

    • Author(s)
      平田尚也、林和花、関野祐子、諫田泰成
    • Organizer
      第84回日本薬理学会
    • Place of Presentation
      横浜(誌上開催)
    • Year and Date
      2011-03-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 乳癌細胞に含まれる癌幹細胞の割合に対するニコチンの影響2011

    • Author(s)
      平田尚也、関野祐子、諫田泰成
    • Organizer
      第10回再生医療学会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-01
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] TGF(3-induced stem cell phenotype in breast cancer cells.2011

    • Author(s)
      Yasunari Kanda, Naoya Hirata, Waka Lin, Yuko Sekino.
    • Organizer
      Keystone Symposia (A8)
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2011-01-22
    • Related Report
      2010 Final Research Report
  • [Presentation] TGFβ-induced stem cell phenotype in breast cancer cells2011

    • Author(s)
      Yasunari Kanda, Naoya Hirata, Waka Lin, Yuko Sekino
    • Organizer
      Keystone Symposia (A8)
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2011-01-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] MCF-7細胞における乳癌幹細胞に対するエストロゲンの影響2011

    • Author(s)
      平田尚也、林和花、関野祐子、諌田泰成
    • Organizer
      第84回日本薬理学会(誌上開催)
    • Related Report
      2010 Final Research Report
  • [Presentation] Role of NADPH oxidase in adipocyte differentiation and obesity2010

    • Author(s)
      Yasunari Kanda
    • Organizer
      EMBO Conference on Cellular Signaling & Molecular Medicine
    • Place of Presentation
      Cavtat, Croatia
    • Year and Date
      2010-05-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Role of NADPH oxidase in adipocyte differentiation and obesity.2010

    • Author(s)
      Yasunari Kanda.
    • Organizer
      EMBO Conference on Cellular Signaling & Molecular Medicine 2nd
    • Place of Presentation
      Cavtat, Croatia
    • Year and Date
      2010-05-22
    • Related Report
      2010 Final Research Report
  • [Presentation] GPR30-mediated signaling pathway in rat vascular smooth muscle cells.2010

    • Author(s)
      Yasunari Kanda.
    • Organizer
      XX World Congress of the ISHR
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2010-05-16
    • Related Report
      2010 Final Research Report
  • [Presentation] GPR30-mediated signaling pathway in rat vascular smooth muscle cells2010

    • Author(s)
      Yasunari Kanda
    • Organizer
      XX World Congress of the ISHR
    • Place of Presentation
      Kyoto
    • Year and Date
      2010-05-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Role of Nox4 in adipocyte differentiation from mesenchymal stem cells.2010

    • Author(s)
      Yasunari Kanda.
    • Organizer
      Keystone Symposia (B4)
    • Place of Presentation
      Keystone, USA
    • Year and Date
      2010-02-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Role of Nox4 in adipocyte differentiation from mesenchymal stem cells2010

    • Author(s)
      諫田泰成
    • Organizer
      Keystone Symposia
    • Place of Presentation
      アメリカ合衆国キーストン
    • Year and Date
      2010-02-17
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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