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Identification of a novel post-translational modification of epidermal growth factor domains and mechanistic analysis of the modification

Research Project

Project/Area Number 21790270
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionNagoya University

Principal Investigator

OKAJIMA Tetsuya  Nagoya University, 医学系研究科, 准教授 (20420383)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
KeywordsNotch受容体 / 糖鎖修飾 / O-結合型糖鎖 / O-GlcNAc / Notch / 糖転移酵素
Research Abstract

Epidermal growth factor (EGF) domains are posttranslationally modified with unique O-linked glycans. The classical types of O-glycans on EGF domains are O-fucose and O-glucose glycans, found on many plasma glycoproteins and signaling molecules, whose biological functions have been demonstrated especially in the context of the Notch signaling pathway. In this study, we have discovered O-GlcNAc modification as a new modification of the EGF domain that occurs on the conserved Ser/Thr residue located between the fifth and sixth cysteine residues within the EGF domains of Notch receptors in Drosophila. Moreover, we have succeeded in the isolation of a new gene, Eogt, responsible for the extracellular O-GlcNAcylation. Characterization of physiological function of Eogt will reveal the new biological roles for O-GlcNAcylation in the extracellular environment.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (17 results)

All 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (3 results) Remarks (2 results)

  • [Journal Article] O-GlcNAc modification of EGF domains of Notch receptors.2010

    • Author(s)
      Sakaidani Y, Furukawa K, Okajima T.
    • Journal Title

      Methods Enzymol. 480

      Pages: 355-373

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] O-GlcNAc modification of the extracellular domain of Notch receptors2010

    • Author(s)
      Okajima T, Furukawa K, Sakaidani Y.
    • Journal Title

      Trends in Glycoscience and Glycotechnology 22

      Pages: 247-255

    • NAID

      10027893118

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of glycosylation in Notch signaling.2010

    • Author(s)
      Stanley, P, Okajima, T.
    • Journal Title

      Current topics in developmental biology 92

      Pages: 131-164

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a Mammary Stromal Fibroblastic Cell Line for In Vitro Studies in Mice of Mammary Adipocyte Differentiation.Biol.2010

    • Author(s)
      Nakatani H, Aoki N, Okajima T, Nadano D, Flint D, Matsuda T.
    • Journal Title

      Reprod. 82(1)

      Pages: 44-53

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of glycosylation in Notch signaling2010

    • Author(s)
      Stanley, P
    • Journal Title

      Current topics in developmental biology

      Volume: 92 Pages: 131-164

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] O-GlcNAc modification of the extracellular domain of Notch receptors2010

    • Author(s)
      Okajima T
    • Journal Title

      Trends in Clycoscience and Glycotechnology

      Volume: 22 Pages: 247-255

    • NAID

      10027893118

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] O-GlcNAc modification of EGF domains of Notch receptors2010

    • Author(s)
      Sakaidani Y
    • Journal Title

      Methods Enzymology

      Volume: 480 Pages: 355-373

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Periodate-resistant carbohydrate epitopes recognized by IgG and IgE antibodies from some of the immunized mice and patients with allergy.2009

    • Author(s)
      Hino S, Matsubara T, Urisu A, Aoki N, Sato C, Okajima T, Nadano D, Matsuda T.
    • Journal Title

      Biochem Biophys Res Commun. 380

      Pages: 632-637

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Glycoconjugate glycosyltransferases.2009

    • Author(s)
      Furukawa K, Tsuchida A, Okajima T, Furukawa K.
    • Journal Title

      Glycoconj.J. 26(8)

      Pages: 987-998

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Periodate-resistant carbohydrate epitopes recognized by IgG and IgE antibodies from some of the immunized mice and patients with allergy.2009

    • Author(s)
      Hino S, et al.
    • Journal Title

      Biochem Biophys Res Commun. 380

      Pages: 632-637

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a Mammary Stromal Fibroblastic Cell Line for In Vitro Studies in Mice of Mammary Adipocyte Differentiation.2009

    • Author(s)
      Nakatani H, et al.
    • Journal Title

      Biol.Reprod. 82

      Pages: 44-53

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glycoconjugate glycosyltransferases.2009

    • Author(s)
      Furukawa K, et al.
    • Journal Title

      Glycoconj. J. 26

      Pages: 987-998

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] OGT-independent O-GlcNAcylation of EGF domains of Notch receptors.2010

    • Author(s)
      岡島徹也
    • Organizer
      内藤カンファレンス
    • Place of Presentation
      湘南
    • Year and Date
      2010-07-28
    • Related Report
      2010 Final Research Report
  • [Presentation] OGT-independent O-GlcNAcylation of EGF domains of Notch receptors2010

    • Author(s)
      岡島徹也
    • Organizer
      内藤カンファレンス
    • Place of Presentation
      湘南国際村
    • Related Report
      2010 Annual Research Report
  • [Presentation] O-GlcNAc modification on EGF domins of Notch receptors.2009

    • Author(s)
      Okajima T.
    • Organizer
      Glycobiology meeting 2009
    • Place of Presentation
      米国サンディエゴ
    • Year and Date
      2009-11-13
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.med.nagoya-u.ac.jp/biochemII/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.med.nagoya-u.ac.jp/biochemII/index.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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