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Analysis of protein aggregation in amyotrophic lateral sclerosis.

Research Project

Project/Area Number 21790275
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionOsaka University

Principal Investigator

MATSUMOTO Ayako  Osaka University, 産業科学研究所, 特任助教(常勤) (60444519)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsスーパーオキシドジスムターゼ / 筋萎縮性側索硬化症 / 凝集体 / 筋萎縮性硬化症 / SOD / オリゴマー / 酵素 / 蛋白質 / 脳神経疾患
Research Abstract

We have explored a mechanism of protein aggregation in amyotrophic lateral sclerosis and found that apo SOD1 and FALS mutant SODs become a substrate for transglutaminase. We could detect soluble oligomeric SODls by high-resolution clear native polyacrylamide gel electrophoresis (hrCN-PAGE). Moreover, hrCN-PAGE can be followed by in-gel activity assay, which revealed enzymatic activities on some oligomeric SODIs from both wild type and FALS mutants.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (5 results)

All 2012 2010 2009

All Presentation (4 results) Book (1 results)

  • [Presentation] 筋萎縮性側索硬化症(ALS)の凝集体形成におけるトランスグルタミナーゼの関与2012

    • Author(s)
      松本紋子・松本明郎・谷口直之
    • Organizer
      トランスグルタミナーゼ研究会
    • Place of Presentation
      神戸
    • Year and Date
      2012-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 筋萎縮性側索硬化症(ALS)の凝集体形成におけるトランスグルタミナーゼの関与2010

    • Author(s)
      松本紋子、松本明郎、谷口直之
    • Organizer
      第13回トランスグルタミナーゼ研究会学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Improved analysis for the oligomeric SOD1s of familial amyotrophic lateral sclerosis and their SOD activities by high resolutionclearnativeelectrophoresis2010

    • Author(s)
      松本紋子・松本明郎、谷口直之
    • Organizer
      第33回日本分子生物学会第83回日本生化学会大会合同大会(BMB2010)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
    • Related Report
      2010 Final Research Report
  • [Presentation] Improved analysis for the oligomeric SOD1s of familial amyotrophic lateral sclerosis and their SOD activities by high resolution clear native electrophoresis2010

    • Author(s)
      松本紋子、松本明郎、谷口直之
    • Organizer
      BMB2010(第33回日本分子生物学会年会、第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report
  • [Book] ALS における酸化ストレスおよび酸化型SOD1の関与(監修:谷口直之、編集:赤池孝章、鈴木敬一郎、内田浩二)(病態解明に迫る活性酸素シグナルと酸化ストレス、分担執筆)2009

    • Author(s)
      藤原範子・松本紋子・谷口直之・鈴木敬一郎
    • Publisher
      羊土社
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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