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Mechanism for interaction between gp91phox and p22phox : two membrane-integrated proteins of the phagocyte NADPH oxidase

Research Project

Project/Area Number 21790283
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionKyushu University

Principal Investigator

MIYANO Kei  Kyushu University, 医学研究院, 学術研究員 (60444783)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
KeywordsNADPHオキシダーゼ / 活性酸素 / Nox2 / gp91^<phox> / 膜タンパク質 / Nox2/gp91^<phox> / Rac / p67^<phox>
Research Abstract

The phagocyte NADPH oxidase, dormant in resting cells, is activated during phagocytosis to produce superoxide, a precursor of microbicidal oxidants. The catalytic core of the phagocyte oxidase is Nox2/gp91^<phox>, a membrane-spaning protein that forms a stable heterodimer with p22^<phox>. In this study, I found that both N- and C-terminal regions play a crucial role in Nox binding to p22^<phox>. Furthermore, I showed that a short region between the TPR and activation domains in p67^<phox> is crucial for activation of Nox2.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (28 results)

All 2011 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (14 results) Book (2 results)

  • [Journal Article] Sumimoto H Assessment of the role for Rho family GTPase in NADPH oxidase activation Methods2011

    • Author(s)
      Miyano K
    • Journal Title

      Mol.Biol. in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of the Role for Rho family GTPases in NADPH oxidase activation2011

    • Author(s)
      Kei Miyano, et al.
    • Journal Title

      Methods Mol.Biol.

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A conserved region between the TPR and activation domains of p67^phox participates in activation of the phagocyte NADPH oxidase2010

    • Author(s)
      Maehara Y, Miyano K, Yuzawa S, Akimoto R, Takeya R, Sumimoto H
    • Journal Title

      J Biol Chem. 285

      Pages: 31435-31445

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Membrane phospholipid metabolism during phagocytosis in human neutrophils2010

    • Author(s)
      Minakami R, Maehara Y, Kamakura S, Kumano O, Miyano K, Sumimoto H
    • Journal Title

      Genes Cells. 15

      Pages: 409-424

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Membrane phospholipid metabolism during phagocytosis in human neutrophils2010

    • Author(s)
      Reiko Minakami, et al.
    • Journal Title

      Genes Cells

      Volume: 15 Pages: 409-423

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A conserved region between the TPR and activation domains of p67^<phox> participates in activation of the phagocyte NADPH oxidase2010

    • Author(s)
      Yuichi Maehara, et al.
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 31435-3445

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 酸化ストレス応答とNoxファミリー2009

    • Author(s)
      宮野佳, 住本英樹
    • Journal Title

      生物資料分析 32

      Pages: 289-296

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The insert region of the Rac GTPases is dispensable for activation of superoxide-producing NADPH oxidases.2009

    • Author(s)
      Miyano K, Koga H, Minakami R, Sumimoto H
    • Journal Title

      Biochem J. 422

      Pages: 373-382

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The domain organization of p67^phox, a protein required for activation of the superoxide-producing NADPH oxidase in phagocytes.2009

    • Author(s)
      Yuzawa S, Miyano K, Honbou K, Inagaki F, Sumimoto H
    • Journal Title

      J Innate Immun. 1

      Pages: 543-555

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Domain Organization of p67^<phox>, a Protein Required for Activation of the Superoxide-Producing NADPH Oxidase in Phagocytes2009

    • Author(s)
      Satoru Yuzawa, et al.
    • Journal Title

      Journal of Innate Immunity 1

      Pages: 543-555

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The insert region of the Rac GTPases is dispensable for activation of superoxide-producing NADPH oxidases2009

    • Author(s)
      Kei Miyano, et al.
    • Journal Title

      Biochemical Journal 422

      Pages: 373-382

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 酸化ストレス応答とNoxファミリー2009

    • Author(s)
      宮野佳, 他
    • Journal Title

      生物試料分析 32

      Pages: 289-296

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 活性酸素生成型NADPHオキシダーゼ(Nox)ファミリーの活性化における膜貫通領域と細胞質領域の役割2010

    • Author(s)
      宮野佳, 住本英樹
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 生体防御に重要な活性酸素生成酵素NADPHオキシダーゼの活性化機構2010

    • Author(s)
      宮野佳, 住本英樹
    • Organizer
      第21回日本生体防御学会学術総会
    • Place of Presentation
      仙台
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Roles for the N-terminal transmembrane and C-terminal cytosolic moieties of Nox-family NADPH oxidases2010

    • Author(s)
      Miyano K, Sumimoto H
    • Organizer
      Gordon Research Conference(Nox family NADPH oxidases)
    • Place of Presentation
      Les Diablerets, the Switzerland
    • Related Report
      2010 Final Research Report
  • [Presentation] Roles for the N-terminal transmembrane and C-terminal cytosolic moieties of Nox-family NADPH oxidases2010

    • Author(s)
      Kei Miyano, et al.
    • Organizer
      Gordon Research Conference (Nox Family NADPH Oxidases)
    • Place of Presentation
      Les Diablerets, the Switzerland
    • Related Report
      2010 Annual Research Report
  • [Presentation] The domain organization of p67^phox, a protein required for activation of the superoxide-producing NADPH oxidase in phagocytes2009

    • Author(s)
      湯澤聡, 宮野佳, 本坊和也, 稲垣冬彦, 住本英樹
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Rac-dependent activation of superoxide-producing NADPH oxidases does not require its insert region2009

    • Author(s)
      宮野佳, 古賀博文, 水上令子, 住本英樹
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] Nox2活性化におけるp67^phoxのドメイン配置と作用機構2009

    • Author(s)
      湯澤聡, 宮野佳, 本坊和也, 稲垣冬彦, 住本英樹
    • Organizer
      第82回日本生化学会大会
    • Related Report
      2010 Final Research Report
  • [Presentation] Racのinsert regionは活性酸素生成酵素NAPDHオキシダーゼ活性化に必要ない2009

    • Author(s)
      宮野佳, 古賀博文, 水上令子, 住本英樹
    • Organizer
      第82回日本生化学会大会
    • Related Report
      2010 Final Research Report
  • [Presentation] スーパーオキシド生成酵素NADPHオキシダーゼの活性化因子p67^phoxの作用機構の解明2009

    • Author(s)
      宮野佳, 前原優一, 湯澤聡, 住本英樹
    • Organizer
      平成21年度日本生化学会九州支部例会
    • Related Report
      2010 Final Research Report
  • [Presentation] スーパーオキシド生成酵素NADPHオキシダーゼの活性化因子p67phoxの作用機構の解明2009

    • Author(s)
      宮野佳, 他
    • Organizer
      平成21年度日本生化学会九州支部例会
    • Place of Presentation
      福岡
    • Related Report
      2009 Annual Research Report
  • [Presentation] 低分子量Gタンパク質Racのinsert regionは活性酸素生成酵素NAPDHオキシダーゼ活性化に必要ない2009

    • Author(s)
      宮野佳, 他
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Annual Research Report
  • [Presentation] Nox2活性化におけるp67^<phox>のドメイン配置と作用機構2009

    • Author(s)
      湯澤聡, 他
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Annual Research Report
  • [Presentation] Rac-dependent activation of superoxide-producing NADPH oxidases doesnot require its insert region2009

    • Author(s)
      宮野佳, 他
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation]2009

    • Author(s)
      湯澤聡, 他
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Book] Front. Gastrointest.Res2011

    • Author(s)
      Sumimoto H, Minakami R, Miyano K
    • Publisher
      In free radical biology in digestive diseases(The Nox Family of NADPH oxidases that Deliberately Produce Reactive Oxygen Species)(S.Karger)
    • Related Report
      2010 Final Research Report
  • [Book] In Free Radical Biology in Digestive Diseases, Front.Gastrointest.Res.2011

    • Author(s)
      Hideki Sumimoto, et al.
    • Total Pages
      176
    • Publisher
      S.Karger
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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