Identification of the mechanisms of the fetal Leydig cell differentiation

• Project/Area Number: 21790284
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kyushu University
• Principal Investigator: SHIMA Yuichi Kyushu University, 医学研究院, 助教 (80425420)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 遺伝子 / ゲノム / 発現制御 / 発生 / 分化 / マイクロアレイ / ライディッヒ細胞 / テストステロン / エンハンサー / 核内受容体 / 内分泌 / トランスジェニックマウス / Ad4BP / SF-1 / 転写

Research Abstract

We have identified fetal Leydig cell-specific enhancer of the Ad4BP/SF-1 gene, and established the mouse lines in which EGFP or Cre is expressed specifically in the fetal Leydig cells. In order to identify mRNA expression profile of the fetal Leydig cells, we performed cell sorting and microarray analyses. In addition, we intercrossed Cre-expressing mice with the SF-1 flox mice, and generated the fetal Leydig cell-specific Ad4BP/SF-1 KO mice. From the analyses of these mice, we have identified that Ad4BP/SF-1 is essential for the function and differentiation of the fetal Leydig cells, and also for the masculinization of the fetuses.

Research Products

• [Journal Article] Methylation of Intronic Enhancers Directs Tissue-Specific Expression of Steroidogenic Factor 1/Adrenal 4 Binding Protein(SF-1/Ad4BP). (2011)
  • Author(s): Hoivik EA, Bjanesoy TE, Mai O, Okamoto S, Minokoshi Y, Shima Y, Morohashi K, Bohem U, Bakke M
  • Journal Title: Endocrinology Epub ahead of print
• [Journal Article] DNA methylation of intronic enhancers directs tissue-specific expression of Steroidogenic Factor 1/Adrenal 4 Binding Protein (SF-1/Ad4BP). (2011)
  • Author(s): Hoivik EA, et al.
  • Journal Title: Endocrinology Volume: (掲載決定)
  • Peer Reviewed
• [Journal Article] エネルギー代謝とその調節機序エネルギー代謝にかかわる因子転写因子Ad4BP/SF-1 (2010)
  • Author(s): 嶋雄一、諸橋憲一郎
  • Journal Title: 日本臨床 68巻増刊号2号 Pages: 200-204
• [Journal Article] Abnormal epithelial cell polarity and ectopic epidermal growth factor receptor(EGFR)expression induced in Emx2 KO embryonic gonads. (2010)
  • Author(s): Kusaka M, Katoh-Fukui Y, Ogawa H, Miyabayashi K, Baba T, Shima Y, Sugiyama N, Sugimoto Y, Okuno Y, Kodama R, Iizuka-Kogo A, Senda T, Sasaoka T, Kitamura K, Aizawa S, Morohashi K
  • Journal Title: Endocrinology. 151(12) Pages: 5893-904
  • NAID: 120005227384
• [Journal Article] Abnormal epithelial cell polarity and ectopic epidermal growth factor receptor (EGFR) expression induced in Emx2 KO embryonic gonads. (2010)
  • Author(s): Kusaka M, et al.
  • Journal Title: Endocrinology Volume: 151 Pages: 5893-5904
  • Peer Reviewed
• [Journal Article] エネルギー代謝とその調節機序 エネルギー代謝に関わる因子 転写因子Ad4BP/SF-1 (2010)
  • Author(s): 嶋雄一、諸橋憲一郎
  • Journal Title: 日本臨床 68(2) Pages: 200-204
• [Presentation] Transcriptomic analysis of fetal Leydig cells (2010)
  • Author(s): 嶋雄一、宮林香奈子、馬場崇、大竹博之、栗原淑子、諸橋憲一郎
  • Organizer: 第33回日本分子生物学会年会ワークショップ「核内受容体研究の最前線」
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2010-12-09
• [Presentation] Transcriptomic analysis of fetal Leydig cells (2010)
  • Author(s): 嶋雄一, 他
  • Organizer: 第33回日本分子生物学会年会(ワークショップ「核内受容体研究の最前線」)
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2010-12-09
• [Presentation] Expression profile of steroidogenic genes in the fetal Leydig cells (2010)
  • Author(s): 宮林香奈子、嶋雄一、馬場崇、大竹博之、栗原淑子、奥野恭史、諸橋憲一郎
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-12-08
• [Presentation] 胎仔型ライディッヒ細胞の生理学的機能の解明 (2010)
  • Author(s): 嶋雄一、馬場崇、宮林香奈子、大竹博之、諸橋憲一郎
  • Organizer: 第10回日本内分泌学会九州地方会
  • Place of Presentation: 鹿児島大学医学部鶴陵会館
  • Year and Date: 2010-08-28
• [Presentation] Transcriptomic analyses of fetal Leydig cell (2010)
  • Author(s): Yuichi Shima, Takashi Baba, Kanako Miyabayashi, Hiroyuki Otake, Yoshiko Kurihara, Ken-ichirou Morohashi
  • Organizer: Gordon Research Conference, Reproductive Tract Biology
  • Place of Presentation: Procter Academy, Andover, NH, USA
• [Presentation] Cell lineage of the fetal and adult Leydig cell (2009)
  • Author(s): 嶋雄一、馬場崇、宮林香奈子、栗原淑子、諸橋憲一郎
  • Organizer: 第32 回日本分子生物学会年会
  • Place of Presentation: 横浜国際展示場
  • Year and Date: 2009-12-10
• [Presentation] 胎仔型ライディッヒ細胞と成獣型ライディッヒ細胞の細胞系譜の解明 (2009)
  • Author(s): 嶋雄一、諸橋憲一郎
  • Organizer: 第17回日本ステロイドホルモン学会
  • Place of Presentation: 九州大学医学部百年講堂
  • Year and Date: 2009-11-14
• [Presentation] CELL LINEAGE OF THE FETAL LEYDIG CELL AND ADULT LEYDIG CELL (2009)
  • Author(s): Yuichi Shima, Ken-ichirou Morohashi
  • Organizer: Fifth international symposium on the bioiogy of vertebrate sex determination
  • Place of Presentation: Royal Kona Resort Kona, Hawaii, USA
• [Presentation] Cell lineage of the fetal Leydig cell and adult Leydig cell (2009)
  • Author(s): 嶋雄一、馬場崇、宮林香奈子、栗原淑子、諸橋憲一郎
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市 パシフィコ横浜
• [Remarks] ホームページ等
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