Analysis of mRNP complex using gene knockin in mammalian cells
Project/Area Number |
21790289
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
General medical chemistry
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Research Institution | Yokohama City University |
Principal Investigator |
YAMASHITA Akio Yokohama City University, 医学研究科, 講師 (20405020)
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Project Period (FY) |
2009 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | 細胞医化学 / 遺伝情報複製 / 転写装置 / 再編 / 制御 |
Research Abstract |
I cloned genomic regions of VEGF, IL6, IL5, IFNbeta and IFNgamma. I constructed IFNbeta gene knockin vector for the insertion of BoxB sequence at downstream of termination codon. Next, I generate cell lines (HeLa TetOff, HeLaTetOnAdvanced, HCT116、BEAS-2B TetOff) which containing a single FRT recombination site. I also constructed the pTRE-Tight-FRT IL-6, IFNbeta, IL28B, and IL-5 full length genes.
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Report
(3 results)
Research Products
(45 results)
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[Journal Article] AAA+ family ATPases, RUVBL1 and RUVBL2, coordinate PIKK family and play critical roles in Nonsense-mediated mRNA decay.2010
Author(s)
Izumi N, Yamashita A (corresponding author), Iwamatsu A, Kurata R, Nakamura H, Saari B, Hirano H, Anderson P, Ohno S
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Journal Title
Science signaling (Science姉妹誌). (3)
Pages: 27-27
Related Report
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[Journal Article] SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.2009
Author(s)
Yamashita A (corresponding author), Izumi N, Kashima I, Ohnishi T, Saari B, Katsuhata Y, Muramatsu R, Morita T, Iwamatsu A, Kurata R, Hachiya T, Hirano H, Anderson P, Ohno S.
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Journal Title
Genes and Development. 23(9)
Pages: 1091-1105
Related Report
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[Journal Article] SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.2009
Author(s)
Yamashita A (Corresponding Author), Izumi N, Kashima I, Ohnishi T, Saari B, Katsuhata Y, Muramatsu R, Morita T, Iwamatsu A, Kurata R, Hachiya T, Hirano H, Anderson P, Ohno S
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Journal Title
Genes and development 23
Pages: 1091-1105
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Peer Reviewed
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[Presentation] THE REMODELING OF MRNA SURVEILLANCE COMPLEX DURING NONSENSE-MEDIATED MRNA DECAY2009
Author(s)
Yamashita A, Izumi N, Okada-Katsuhata Y, Kutsuzawa K, Muramatsu R, Sarri B, Iwamatsu A, Hirano H, Hirahata F, Anderson P, Ohno S.
Organizer
Cold spring harbor laboratory meeting 2009mRNA processing
Place of Presentation
New York。 USA
Year and Date
2009-08-20
Related Report
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[Presentation] THE REMODELING OF MRNA SURVEILLANCE COMPLEX DURING NONSENSE-MEDIATED MRNA DECAY.2009
Author(s)
Yamashita A, Izumi N, Okada-Katsuhata Y, Kutsuzawa K, Muramatsu R, Sarri B, Iwamatsu A, Hirano H, Hirahata F, Anderson P, Ohno S.
Organizer
Cold spring harbor laboratory meeting 2009 mRNA processing
Place of Presentation
New York
Related Report
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