analysis of human amniotic membrane-derived cells related to hematopoiesis-supportive functions

• Project/Area Number: 21790297
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SUDO Kazuhiro 独立行政法人理化学研究所, 細胞材料開発室, 協力研究員 (10392002)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 羊膜細胞 / 臍帯細胞 / 造血幹細胞 / 造血支持能 / 共培養 / 共移植 / ヒト臍帯血CD34陽性細胞 / ヒト臍帯由来細胞 / ヒト羊膜由来細胞 / 長期共培養 / サイトカイン産生 / 臍帯血CD34陽性細胞 / 移植 / サイトカイン / 羊膜由来細胞 / 臍帯血 / CD34陽性細胞

Research Abstract

Co-culture of cord blood CD34^+cells with human amniotic membrane(HAM)-or human umbilical cord(HUC)-derived cells caused increase of CD45^+hematopoietic cells. however, percentage of CD34^+cells was decreased with time of co-culture. Colony-forming cells(CFCs) were maintained in co-culture at least for 5 weeks. The chimerisms of human CD45^+cells in NOD/SCID mice were significantly higher when HAM-or HUC-derived cells were co-transplanted with CD34+cells than when CD34^+cells were transplanted alone. Those results strongly suggest that HAM-and HUC-derived cells have hematopoiesis-supportive functions.

Research Products

• [Presentation] ヒト臍帯および羊膜由来細胞の造血支持能の比較 (2010)
  • Author(s): 須藤和寛、高森靖、菅野恵、金指幹元、中村幸夫
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] ヒト臍帯および羊膜由来細胞の造血支持能の比較 (2010)
  • Author(s): 須藤和寛
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 日本、神戸
  • Year and Date: 2010-12-08