|Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
We attempted to create a transgenic rabbit as a tool to analyze the function of apolipoprotein(apo) CIII on lipid metabolism. 2727 ova were microinjected with human apoCIII expression DNA construct. 2276 embryos were transferred into 87 recipient rabbits, and 24 offspring were born. As a result of PCR analysis, no integration of human apoCIII gene was recognized in the offspring.