• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanism of epithelial-mesenchymal transition in renal cell carcinomas

Research Project

Project/Area Number 21790365
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionKeio University

Principal Investigator

MIKAMI Shuji  Keio University, 医学部, 助教 (20338180)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords上皮-間葉転換 / 腎細胞癌 / Snail / 転移 / 浸潤 / 紡錘形細胞癌 / Snai1
Research Abstract

The transcriptional factor Snail has been proposed as an important mediator of epithelial-mesenchymal transition because of its role in down-regulation of E-cadherin and up-regulation of matrix metalloproteinases (MMPs). Ninety-seven primary RCCs were analyzed for the protein expression of Snail and E-cadherin by immunohistochemistry. The expression level of Snail was significantly higher in high-grade RCCs than in low-grade RCCs. The patients with Snail-high/E-cadherin-low RCCs had poor prognosis. Targeting the Snail expression in 786-O cells with siRNA caused down-regulation of the gene expression of Snail, MMP9, but up-regulated the E-cadherin. Invasion of the cells through Matrigel in vitro was inhibited under this condition. In conclusions, these data suggest that Snail plays an important role in invasion and metastasis, and that silencing the gene may be a potential therapeutic target in RCCs.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report

Research Products

(3 results)

All 2011 2010 2009

All Journal Article Presentation

  • [Journal Article] Expression of Snail and Slug in renal cell carcinoma : E-cadherin repressor Snail is associated with cancer invasion and prognosis.2011

    • Author(s)
      Shuji Mikami, Ken-ichi Katsube, Mototsugu Oya, Masaru Ishida, Takeo Kosaka, Ryuichi Mizuno, Makio Mukai, Yasunori Okada.
    • Journal Title

      Laboratory Investigation in press.

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] 腎細胞癌におけるSnail発現の意義および機能解析2010

    • Author(s)
      三上修治, 勝部憲一, 大家基嗣, 石田勝, 小坂威男, 水野隆一, 向井万起男, 岡田保典
    • Organizer
      日本病理学会総会
    • Place of Presentation
      東京
    • Year and Date
      2010-04-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 腎細胞癌におけるSnail発現と臨床病理学的因子および予後との関連2009

    • Author(s)
      三上修治、勝部憲一、大家基嗣、石田勝、小坂威雄、向井万起男、岡田保典
    • Organizer
      第98回日本病理学会総会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2009-05-02
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi