Molecular mechanism of epithelial-mesenchymal transition in renal cell carcinomas
| Project/Area Number |
21790365
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Keio University |
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Principal Investigator |
MIKAMI Shuji Keio University, 医学部, 助教 (20338180)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 上皮-間葉転換 / 腎細胞癌 / Snail / 転移 / 浸潤 / 紡錘形細胞癌 / Snai1 |
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| Research Abstract |
The transcriptional factor Snail has been proposed as an important mediator of epithelial-mesenchymal transition because of its role in down-regulation of E-cadherin and up-regulation of matrix metalloproteinases (MMPs). Ninety-seven primary RCCs were analyzed for the protein expression of Snail and E-cadherin by immunohistochemistry. The expression level of Snail was significantly higher in high-grade RCCs than in low-grade RCCs. The patients with Snail-high/E-cadherin-low RCCs had poor prognosis. Targeting the Snail expression in 786-O cells with siRNA caused down-regulation of the gene expression of Snail, MMP9, but up-regulated the E-cadherin. Invasion of the cells through Matrigel in vitro was inhibited under this condition. In conclusions, these data suggest that Snail plays an important role in invasion and metastasis, and that silencing the gene may be a potential therapeutic target in RCCs.
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Report
(3 results)
Research Products
(3 results)
