Analyses of functions of deregulated miRNAs in renal carcinoma

• Project/Area Number: 21790389
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Oita University
• Principal Investigator: NAKADA Chisato Oita University, 医学部, 助教 (60379625)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: microRNA / 腎細胞癌

Research Abstract

It has recently been reported that microRNAs (miRNAs), non-protein-coding small RNAs comprising about 22 nucleotides, are aberrantly expressed in certain carcinomas and play oncogenic or tumor-suppressive roles in carcinoma cells. The aim of this project is to clarify the functions of the miRNAs aberrantly expressed in clear cell renal cell carcinoma (CCC), and to identify miRNAs associated with carcinogenesis and/or cancer progression.
Three miRNAs are found deregulated in CCC, but the mechanism and biological consequences of their deregulation are poorly understood. MiR-200c and miR-141 are significantly downregulated in CCC. Ectopic expression of these miRNAs induced growth suppression of renal carcinoma cell lines. Ectopic expression of miR-210, which is upregulated in CCC, caused accumulation at G2/M phase of the cell cycle associated with multipolar spindle, suggesting that miR-210 overexpression may trigger an event that hinders normal cell division.

Research Products

• [Journal Article] Overexpression of miR-210 (2011)
  • Author(s): Chisato Nakada, Yoshiyuki Tsukamoto, Keiko Matsuura, Tung Lam Nguyen, Naoki Hijiya, Tomohisa Uchida, Fuminori Sato, Hiromitsu Mimata, Seto Masao, Masatsugu Moriyama
  • Journal Title: a downstream target of HIF1α, causes centrosome amplification in renal carcinoma cells J Pathol 224 Pages: 280-288
  • Peer Reviewed
• [Journal Article] Overexpression of miR-210, a downstream target of HIF1α, causes centrosome amplification in renal carcinoma cells (2011)
  • Author(s): Nakada C, et al.
  • Journal Title: The Journal of Pathology Volume: 224(Published online)(4 Apr)
  • Peer Reviewed
• [Journal Article] Masatsugu Moriyama MicroRNA-375 Is Downregulated in Gastric Carcinomas and Regulates Cell Survival by Targeting PDK1 and 14-3-3ζ (2010)
  • Author(s): Yoshiyuki Tsukamoto, Chisato Nakada, Tsuyoshi Noguchi, Masato Tanigawa, Lam Tung Nguyen, Tomohisa Uchida, Naoki Hijiya, Keiko Matsuura, Toshio Fujioka, Masao Seto
  • Journal Title: Cancer Res 70(6) Pages: 2339-2349
  • Peer Reviewed
• [Journal Article] MicroRNA-375 Is Downregulated in Gastric Carcinomas and Regulates Cell Survival by Targeting PDK1 and 14-3-3ζ (2010)
  • Author(s): Tsukamoto Y, Nakada C, et. al.
  • Journal Title: Cancer Research 70 Pages: 2339-2349
  • Peer Reviewed
• [Presentation] 胃癌で発現低下するmiR-375はPDK1と14-3-3ζを標的として細胞生存を制御する (2010)
  • Author(s): 塚本善之、中田知里, ほか
  • Organizer: 第69回 日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-22
• [Presentation] ほか 胃癌で発現低下するmiR-375はPDK1と14-3-3ζを標的として細胞生存を制御する (2010)
  • Author(s): 塚本善之、中田知里
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-02