Combined analysis of tertiary structure and function(s) of Clostridium botulinum type C and D phospholipase C
| Project/Area Number |
21790421
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | University of Miyazaki (2010)
Okayama University (2009) |
|---|
Principal Investigator |
SAKAGUCHI Yoshihiko University of Miyazaki, IR推進機構, 助教 (70403491)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | ボツリヌス菌 / ホスホリパーゼC / ウエルシュ菌α毒素 / 卵黄活性 / 溶血活性 / ホスファチジルコリン / スフィンゴミエリン / リポソーム |
|---|
| Research Abstract |
Clostridium botulinum cultures are classified into seven types, types A to G, based on the antigenicity of the neurotoxins produced. Of these seven types, only types C and D produce phospholipase C (lecithinase, PLC) in addition to the neurotoxin. Phospholipase of C. perfringens is known as α-toxin, which is the best characterized of all clostridial phospholipases and is an important agent of gas gangrene. The toxin, which exhibits phospholipase C and sphingomyelinase (SMase) activities, causes hemolysis, necrosis, and death. Here we determined the PLC genes of C. botulinum type C and D (Cbotα and Dbotα) by using inverse PCR and primer walking, and expressed as a GST-fusion protein. We compare the enzymatic and biological activities (egg-yolk activity, hemolysis, and mouse lethality) of the purified protein of C. perfringens α-toxin, Cbotα, and Dbotα.
|
Report
(3 results)
Research Products
(23 results)
-
[Journal Article] A new cytosolicpathway from a Parkinsondisease-associated kinase, BRPK/PINK1:activation of AKT via MTORC2.2011
Author(s)
Murata, H., Sakaguchi, M., Jin, Y., Sakaguchi, Y., Futami, J., Yamada, H., Kataoka, K., Huh, NH.
-
Journal Title
J.Biol.Chem. 286(9)
Pages: 7182-7189
Related Report
Peer Reviewed
-
[Journal Article] A new cytosolic pathway from a Parkinson disease-associated kinase, BRPK/PINK1 : activation of AKT via mTORC22011
Author(s)
Murata, H., Sakaguchi, M., Jin, Y., Sakaguchi, Y., Futami, J.I., Yamada, H., Kataoka, K., Huh, N.H.
-
Journal Title
J.Biol.Chem.
Volume: 286
Pages: 7182-7189
Related Report
Peer Reviewed
-
[Journal Article] Transcriptionalregulation of a brown adipocyte-specificgene, UCP1, by KLF11 and KLF15.2010
Author(s)
Yamamoto, K., Sakaguchi, M., Medina, RJ., Niida A., Sakaguchi, Y., Miyazaki, M., Kataoka, K., Huh, NH.
-
Journal Title
Biochem.Biophys.Res.Commun. 400(1)
Pages: 175-180
Related Report
Peer Reviewed
-
[Journal Article] Transcriptional regulation of a brown adipocyte-specificgene, UCP1, by KLF11 and KLF152010
Author(s)
Yamamoto, K., Sakaguchi, M., Medina, R.J., Niida, A., Sakaguchi, Y., Miyazaki, M., Kataoka, K., Huh, N.H.
-
Journal Title
Biochem.Biophys.Res.Commun.
Volume: 400
Pages: 175-180
Related Report
Peer Reviewed
-
[Journal Article] Binding and internalization of Clostridium botulinuR C2 toxin2009
Author(s)
Nagahama.M., Hagiyama.T., Kojima.T., Aoyanagi, K., Takahashi, C., Oda, M., Sakaguchi.Y., Oguma.K., Sakurai, J.
-
Journal Title
Infection and Immunity 77(11)
Pages: 51395148-51395148
Related Report
Peer Reviewed
-
-
-
-
-
-
-
[Presentation] ボツリヌス菌ホスホリパーゼCの酵素活性と生物活性2010
Author(s)
阪口義彦, 小田真隆, Ni Nengah Dwi Fatmawati, 山本由弥子, 唐澤忠宏, 小林敬子, 永浜政博, 櫻井純, 小熊恵二
Organizer
BMB2010,第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
Place of Presentation
神戸ポートアイランド(神戸)
Related Report
-
-
-
-
-
-
-
-
-
-
[Presentation] C型とD型ボッリヌス菌ボスホリパーゼCの酵素活性と生物活性2009
Author(s)
阪口義彦, 小田真隆, 山本由弥子, 難波ひかる, 馬少博, 張凱, 田村臣哉, 唐澤忠宏, 小林敬子, 永浜政博, 櫻井純, 小熊恵二
Organizer
第32回 日本分子生物学会年会
Place of Presentation
パシフィコ横浜(横浜)
Related Report
-
