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Inhibitory effect of NaCl on the production of extracellular proteins by Aeromonas spp.

Research Project

Project/Area Number 21790422
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Bacteriology (including Mycology)
Research InstitutionOkayama University

Principal Investigator

TAKAHASHI Eizo  岡山大学, 大学院・医歯薬学総合研究科, 助教 (70379733)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords病原性 / 病原毒素 / アエロモナス(エロモナス) / プロテアーゼ / リパーゼ / NaCl / Aeromonas / 菌体外毒素 / 産生抑制 / lysR転写調節因子 / 食塩 / Aeromonas sobria / メタロプロテアーゼ / 転写調節 / 菌体外リパーゼ
Research Abstract

Aeromonas, which is a causative agent of diarrhea of adults and children, could not produce active metalloprotease and lipase in the medium containing 3% NaCl, though it could produce them under physiological condition. In this study, we constructed the mutant strain in which the gene encoding LysR type transcriptional factor is deleted and examined whether the deletion of LysR affects the production of metalloprotease. The results show that LysR type transcriptional factor is not involved in production of metalloprotease. Additionally, we found the lipase gene is transcribed in the medium containing 3% NaCl as well as in the medium 0. 5% NaCl, though production of lipase is reduced under salty condition. And we showed that the lipase produced slightly in the medium containing 3% NaCl is denatured.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (44 results)

All 2012 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (33 results)

  • [Journal Article] Hydrolysable tannins isolated from Syzygium aromaticum : structure of a new c-glucosidic ellagitannin and spectral features of tannins with a tergalloyl group2012

    • Author(s)
      Li-Ming Bao, et al
    • Journal Title

      Heterocycles

      Volume: 85(2) Issue: 2 Pages: 365-381

    • DOI

      10.3987/com-11-12392

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a sensitive rRNA-targeted reverse transcription-quantitative polymerase chain reaction for detection of Vibrio cholerae/mimicus, V.parahaemolyticus/alginolyticus and Campylobacter jejuni/coli2012

    • Author(s)
      Takashi Kurakawa, et al
    • Journal Title

      Microbiology and Immunology

      Volume: 56(1) Issue: 1 Pages: 10-20

    • DOI

      10.1111/j.1348-0421.2011.00405.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of biosynthesis of metalloprotease of Aeromonas sobria by sodium chloride in themedium2011

    • Author(s)
      Eizo Takahashi, et al
    • Journal Title

      Microbiology and Immunology

      Volume: Vol.55 No.1 Pages: 60-65

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of biosynthesis of metalloprotease of Aeromonas sobria by sodiumchloride in the medium2011

    • Author(s)
      Eizo Takahashi, et al.
    • Journal Title

      Microbiology and Immunology

      Volume: 55(1) Pages: 60-65

    • NAID

      10031121441

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maturation pathway of metalloprotease produced by Aeromonas sobria2010

    • Author(s)
      Eizo Takahashi, et al
    • Journal Title

      Microbiology and Immunology

      Volume: Vol.54 No.10 Pages: 596-605

    • NAID

      10029545874

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Maturation pathway of metalloprotease produced by Aeromonas sobria2010

    • Author(s)
      Eizo Takahashi, et al.
    • Journal Title

      Microbiology and Immunology

      Volume: 54(10) Pages: 596-605

    • NAID

      10029545874

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Candida属真菌に対する各種精油および精油成分の抗真菌作用,およびAmphotericinBの抗真菌作用に及ぼす影響2010

    • Author(s)
      野崎阿季子
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 130(6) Pages: 895-902

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The carboxy-terminal tail of Aeromonas sobria serine protease is associated with the chaperone2009

    • Author(s)
      Hidetomo Kobayashi, Arisa Tateishi, Hideaki Tsuge, Eizo Takahashi, Keinosuke Okamoto, Hiroyasu Yamanaka
    • Journal Title

      Microbiology and Immunology 53(12)

      Pages: 647-647

    • NAID

      10027687949

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] グァバ飲料およびグァバ葉に含まれるタンニンおよび関連ポリフェノールの食中毒細菌に及ぼす抗菌作用2009

    • Author(s)
      山中文, 波多野力, 伊藤秀之, 谷口抄子, *高橋栄造, 岡本敬の介
    • Journal Title

      機能性食品と薬理栄養 5(4)

      Pages: 217-222

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production and properties of lipase of Aeromonas sobria

    • Author(s)
      Eizo Takahashi, et al
    • Journal Title

      Microbiology and Immunology

      Volume: (in press)

    • NAID

      40019309951

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production and properties of lipase of Aeromonas sobria

    • Author(s)
      Eizo Takahashi, et al
    • Journal Title

      Microbiology and Immunology

      Volume: (In press)

    • NAID

      40019309951

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Characterization of elastolytic metalloprotease produced by Aeromonas hydrophila2012

    • Author(s)
      Eizo Takahashi
    • Organizer
      Asian-African Research Forum
    • Place of Presentation
      神戸国際会議場(神戸)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Aeromonas trotaの産生するセリンプロテアーゼの性状解析2012

    • Author(s)
      高橋栄造
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 腸炎ビブリオの鉄獲得系と生体内増殖能の検討、及び抗ピブリオフェリン受容体モノクローナル抗体の作成2012

    • Author(s)
      中尾浩史
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Related Report
      2011 Annual Research Report
  • [Presentation] アエロモナスの菌体外毒素産生に及ぼす食塩の影響と生息環境との関係2011

    • Author(s)
      高橋栄造
    • Organizer
      第二回愛媛微生物学ネットワークフォーラム
    • Place of Presentation
      松山
    • Year and Date
      2011-11-26
    • Related Report
      2011 Final Research Report
  • [Presentation] アエロモナスの菌体外毒素産生に及ぼす食塩の影響と生息環境との関係2011

    • Author(s)
      高橋栄造
    • Organizer
      第二回愛媛微生物学ネットワークフォーラム
    • Place of Presentation
      松山大学(松山)(招待講演)
    • Year and Date
      2011-11-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inhibitory effect of Salt on the Production of metalloprotease by Aeromonas sobria2011

    • Author(s)
      Eizo Takahashi
    • Organizer
      IUMS2011
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] Production and characterization of lipase produced by Aeromonas sobria2011

    • Author(s)
      Eizo Takahashi
    • Organizer
      The 45th Annual joint meeting on cholera and other bacterialenteric infections
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] Aeromonas trotaの産生する病原因子に関する研究2011

    • Author(s)
      高橋栄造
    • Organizer
      第58回トキシンシンポジウム
    • Place of Presentation
      順天堂大学(東京)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inhibitory effect of Salt on the Production of metalloprotease by Aeromonas sobria2011

    • Author(s)
      Eizo Takahashi
    • Organizer
      IUMS2011
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Aeromonas trotaの産生するプロテアーゼに関する研究2011

    • Author(s)
      高橋栄造
    • Organizer
      第64回日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山大学(岡山)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Aeromonas sobriaの産生するホスホリパーゼCの精製及び性状解析2011

    • Author(s)
      伊藤英伸
    • Organizer
      第50回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      サンポートホール高松(高松)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Studies on elastolytic activity of the metalloprotease produced by Aeromonas hydrophila2011

    • Author(s)
      Eizo Takahashi
    • Organizer
      The 46th Annual joint meeting on cholera and other bacterial enteric infections
    • Place of Presentation
      Kolkata, India
    • Related Report
      2011 Annual Research Report
  • [Presentation] エラスチン分解に関与するアエロモナスのメタロプロテアーゼの性状解析2010

    • Author(s)
      高橋栄造
    • Organizer
      第57回トキシンシンポジウム
    • Place of Presentation
      長浜ロイヤルホテル(滋賀県長浜市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] A.hydrophilaメタロプロテアーゼのエラスチン分解活性に関する解析2010

    • Author(s)
      高橋栄造
    • Organizer
      第63回 日本細菌学会中国・四国支部総会
    • Place of Presentation
      松山大学(松山市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 腸炎ビブリオのvibrioferrinを介する鉄獲得系と病原性2010

    • Author(s)
      山科竜一
    • Organizer
      第63回日本細菌学会中国・四国支部総会
    • Place of Presentation
      松山大学(松山市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Aeromonas sobriaの産生する菌体外リパーゼの性状解析2010

    • Author(s)
      伊藤英伸
    • Organizer
      第49回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      米子コンベンションセンター(米子市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] アエロモナス菌体外リパーゼの産生とその多様性2010

    • Author(s)
      本田裕太
    • Organizer
      第49回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      米子コンベンションセンター(米子市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Maturation pathway and Activities of metalloprotease of Aeromonas2010

    • Author(s)
      Eizo Takahashi
    • Organizer
      Asian-African Research Forum
    • Place of Presentation
      Melia Hotel (Hanoi, Vietnam)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Production and characterization of lipase produced by Aeromonas sobria2010

    • Author(s)
      Eizo Takahashi
    • Organizer
      The 45th Annual joint meeting on cholera and other bacterial enteric infections
    • Place of Presentation
      Kyoto Univ.(Kyoto, Japan)
    • Related Report
      2010 Annual Research Report
  • [Presentation] アエロモナスの菌体外リパーゼの性状解析2010

    • Author(s)
      高橋栄造
    • Organizer
      日本薬学会 第130年会
    • Place of Presentation
      岡山コンベンションセンター他(岡山市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] アエロモナスのメタロプロテアーゼの性状解析2010

    • Author(s)
      安倍武海
    • Organizer
      日本薬学会 第130年会
    • Place of Presentation
      岡山コンベンションセンター他(岡山市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Aeromonas hydrophilaのエラスチン分解活性に関与するメタロプロテアーゼの遺伝学的解析2010

    • Author(s)
      立石亜里紗
    • Organizer
      日本薬学会 第130年会
    • Place of Presentation
      岡山コンベンションセンター他(岡山市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Aeromonasに対するポリフェノールのプロテアーゼ活性抑制作用2010

    • Author(s)
      石田有佳里
    • Organizer
      日本薬学会 第130年会
    • Place of Presentation
      岡山コンベンションセンター他(岡山市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] アルキル化抗がん剤の変異特性2010

    • Author(s)
      山田よう子
    • Organizer
      日本薬学会 第130年会
    • Place of Presentation
      岡山コンベンションセンター他(岡山市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] アエロモナスの産生する菌体外リパーゼの性状解析2009

    • Author(s)
      高橋栄造
    • Organizer
      第56回毒素シンポジウム
    • Place of Presentation
      アルモニーテラッセ(岐阜)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Aeromonas sobriaの産生する菌体外リパーゼの性状解析2009

    • Author(s)
      高橋栄造
    • Organizer
      第62回 日本細菌学会中国・四国支部総会
    • Place of Presentation
      広島大学霞キャンパス広仁会館(広島市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] アエロモナスの産生するメタロプロテアーゼの性状解析2009

    • Author(s)
      安倍武海
    • Organizer
      第48回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      アスティ徳島(徳島市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] A.hydrophilaのメタロプロテアーゼの多様性とエラスチン分解活性2009

    • Author(s)
      立石亜里紗
    • Organizer
      第48回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      アスティ徳島(徳島市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 日本およびインド環境水中に生息するアエロモナスの病原性遺伝子の保有状況2009

    • Author(s)
      村田公平
    • Organizer
      第48回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      アスティ徳島(徳島市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 腸炎ビブリオのシデロフォア,vibrioferrinを介した鉄獲得系と病原性2009

    • Author(s)
      山田真人
    • Organizer
      第48回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      アスティ徳島(徳島市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Candida属真菌に作用する天然物質の探索2009

    • Author(s)
      野崎阿季子
    • Organizer
      第48回日本薬学会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      アスティ徳島(徳島市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] タンパク質共存下にポリフェノールが形成する複合体およびその作用2009

    • Author(s)
      野崎阿季子
    • Organizer
      第3回食品薬学シンポジウム
    • Place of Presentation
      東大阪市
    • Related Report
      2009 Annual Research Report
  • [Presentation] アエロモナスの菌体外毒素産生と食塩濃度の関係2009

    • Author(s)
      高橋栄造
    • Organizer
      第43回 腸炎ビブリオシンポジウム
    • Place of Presentation
      岡山大学自然科学研究科(岡山市)
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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