Mechanism of mycobacterial growth using host hyaluronan and novel antitubercular drug development targeting mycobacterial hyaluronidase.

• Project/Area Number: 21790426
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka City University
• Principal Investigator: NIKI Mamiko Osaka City University, 大学院・医学研究科, 助教 (20438229)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 結核ビアルロン酸抗結核薬 / 結核 / ヒアルロン酸 / 抗結核薬

Research Abstract

We found that mycobacteria can grow utilizing hyaluronan (HA) as a carbon source. The expression of HA synthase in the was increased after infection of M. tuberculosis. We showed HAase inhibitor suppressed growth of mycobacteria in vivo.

Research Products

• [Journal Article] Effects of non-iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis. (2011)
  • Author(s): Yukitake H, Naito M, Sato K, Shoji M, Ohara N, Yoshimura M, Sakai E, Nakayama K.
  • Journal Title: Microb Immun 55(3) Pages: 141-153
  • NAID: 10031121453
  • Peer Reviewed
• [Journal Article] Changes in salivary antimicrobial peptides, immunoglobulin A and cortisol after prolonged strenuous exercise. (2011)
  • Author(s): Usui T, Yoshikawa T, Orita K, Ueda SY, Katsura Y, Fujimoto S, Yoshimura M.
  • Journal Title: Eur J Appl Physiol
  • Peer Reviewed
• [Journal Article] Effects of non-iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis. (2011)
  • Author(s): Yukitake, H., M.Yoshimura
  • Journal Title: Microb.Immunol. Volume: 55 Pages: 141-153
  • NAID: 10031121453
  • Peer Reviewed
• [Journal Article] Tetratricopeptide repeat protein-associated proteins contribute to the virulence of Porphyromonas gingivalis. (2010)
  • Author(s): Kondo Y, Ohara N, Sato K, Yoshimura M, Yukitake H, Naito M, Fujiwara T, Nakayama K.
  • Journal Title: Infect Immun. 78(6) Pages: 2846-2856
  • NAID: 120006984317
  • Peer Reviewed
• [Journal Article] Changes in salivary antimicrobial peptides, immunoglobulin A and cortisol after prolonged strenuous exercise. (2010)
  • Author(s): Usui, T., M.Yoshimura, et al.
  • Journal Title: Eur.J.Appl.Physiol.
  • Peer Reviewed
• [Journal Article] Tetratricopeptide repeat protein-associated proteins contribute to the virulence of Porphyromonas gingivalis. (2010)
  • Author(s): Kondo, Y., M.Yoshimura, et al.
  • Journal Title: Infect.Immun. Volume: 78 Pages: 2846-2856
  • NAID: 120006984317
  • Peer Reviewed
• [Journal Article] Mycobacteria exploit host hyaluronan for efficient extracellular replication. (2009)
  • Author(s): Hirayama Y, Yoshimura M, Ozeki Y, Sugawara I, Udagawa T, Mizuno S, Itano N, Kimata K, Tamaru A, Ogura H, Kobayashi K, Matsumoto S.
  • Journal Title: PLos Pathog. 5(10)
  • Peer Reviewed
• [Journal Article] Hydrogen sulfide production from cysteine and homocysteine by periodontal and oral bacteria. (2009)
  • Author(s): Yoshida A, Yoshimura M, Ohara N, Yoshimura S, Nagashima S, Takehara T, Nakayama K.
  • Journal Title: J.Periodontol. 80(11) Pages: 1845-1851
  • Peer Reviewed
• [Journal Article] Mycobacterium avium complex organisms predominantly colonize in the bathtub inlets of patients'bathrooms. (2009)
  • Author(s): Nishiuchi Y, Tamura A, Kitada S, Taguri T, Matsumoto S. Tateishi Y, Yoshimura M, Ozeki Y, Matsumura N, Ogura H, Maekura R.
  • Journal Title: Jpn J Infect Dis. 62(3) Pages: 182-186
  • Peer Reviewed
• [Journal Article] Virulence of Mycobacterium avium complex strains isolated from immunocompetent patients. (2009)
  • Author(s): Tateishi Y, Hirayama Y, Ozeki Y, Nishiuchi Y, Yoshimura M, Kang J, Shibata A, Hirata K, Kitada S, Maekura R, Ogura H, Kobayashi K, Matsumoto S.
  • Journal Title: Microb Pathog. 46(1) Pages: 6-12
  • Peer Reviewed
• [Journal Article] Mycobacteria exploit host hyaluronan for efficient extracellular replication (2009)
  • Author(s): Hirayama, Y., M.Yoshimura, et al.
  • Journal Title: Plos Pathog 5
  • Peer Reviewed
• [Journal Article] Hydrogen sulfide production from cysteine and homocysteine by periodontal and oral bacteria (2009)
  • Author(s): Yoshida, A., M.Yoshimura, et al.
  • Journal Title: J Periodontol 80 Pages: 1845-51
  • Peer Reviewed
• [Journal Article] Virulence of Mycobacterium avium complex strains isolated from immunocompetent patients. (2009)
  • Author(s): Tateishi, Y., M.Yoshimura, et al.
  • Journal Title: Microb Pathog 46 Pages: 6-12
  • Peer Reviewed
• [Journal Article] Mycobacterium avium complex organisms predominantly colonize in the bathtub inlets of patients' bathrooms. (2009)
  • Author(s): Nishiuchi, Y., M.Yoshimura, et al.
  • Journal Title: Jpn J Infect Dis 62 Pages: 182-6
  • Peer Reviewed
• [Presentation] 休眠時における抗酸菌の遺伝子発現解析 (2009)
  • Author(s): 吉村満美子
  • Organizer: 第62回日本細菌学会関西支部総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-14
• [Presentation] 抗酸菌の休眠誘導と遺伝子発現解析 (2009)
  • Author(s): 吉村満美子
  • Organizer: 第7回感染症沖縄フォーラム
  • Place of Presentation: 沖縄
  • Year and Date: 2009-02-11