Screening for a new meningococcal pathogenic factor by STM

• Project/Area Number: 21790435
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TAKAHASHI Hideyuki 国立感染症研究所, 細菌第一部, 主任研究官 (60321866)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 感染症 / 微生物 / 髄膜炎菌 / Neisseria meningitidis / 病原因子 / Signature Tag Mutagenesis / トランスポゾン変異 / 変異株 / signature tag mutagenesis

Research Abstract

I constructed a transposon-inserted meningococcal mutant library by STM and screened adhesion or invasion-defective mutants to human brain microvasocular endothelial cells. By the 5th steps of screening, 31 mutants were finally selected.

Research Products

• [Journal Article] Meningococcal internalization into human endothelial and epithelial cell-s is triggered by the influx of extracellular L-glutamate via GltT L-glutamate ABC transporter in Neisseria meningitidis (2011)
  • Author(s): H. Takahashi, K. S. Kim and H. Watanabe
  • Journal Title: Infection and Immunity Volume: 79 Issue: 1 Pages: 380-392
  • DOI: 10.1128/iai.00497-10
  • Peer Reviewed
• [Journal Article] Meningococcal internalization into human endothelial and epithelial cells is triggered by the influx of extracellular L-glutamate via GltT L-glutamate ABC transporter in Neisseria meningitides (2011)
  • Author(s): H.Takahashi, K.S.Kim, H.Watanabe
  • Journal Title: Infection and Immunity Volume: 79 Pages: 380-392
  • Peer Reviewed
• [Journal Article] Immunomoduatory effects of Yerisinia pestis Lipopolysaccharides on human Macrophages (2010)
  • Author(s): M. Matsuura, H. Takahashi, H. Watanabe and K. Kawahara
  • Journal Title: Clinical and Vaccine Immunology Volume: 17 Issue: 1 Pages: 49-55
  • DOI: 10.1128/cvi.00336-09
  • Peer Reviewed
• [Journal Article] Immunomoduatory effects of Yerisinia pestis Lipopolysaccharideson human Macrophages (2010)
  • Author(s): M. Matsuura, H. Takahashi, H. Watanabe, K. Kawahara
  • Journal Title: Clinical and Vaccine Immunology 17 Pages: 49-55
  • Peer Reviewed
• [Presentation] 髄膜炎菌の線毛のマイナータンパクPilVの宿主細胞感染における遺伝学的及び物理化学的手法を用いた機能解析 (2012)
  • Author(s): 高橋英之、大西真
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-27
• [Presentation] 髄膜炎菌の線毛のマイナータンパクPilVの宿主細胞感染における遺伝学的及び物理化学的手法を用いた機能解析 (2012)
  • Author(s): 高橋英之、大西真
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-27
• [Presentation] 国内分離された髄膜炎菌株の分子役学的解析とそれらを用いたNeisseria meningitidisの病原因子の解析 (2009)
  • Author(s): 高橋英之
  • Organizer: 第92回細菌学会関東支部会シンポジウム
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2009-11-08
• [Presentation] 国内分離された髄膜炎菌株の分子役学的解析とそれらを用いたNeisseria menlngitidisの病原因子の解析 (2009)
  • Author(s): 高橋英之
  • Organizer: 第92回細菌学会関東支部会シンポジウム
  • Place of Presentation: 東京都文京区東京医科歯科大学5号館講堂
  • Year and Date: 2009-11-05
• [Presentation] 細菌感染時の髄膜炎菌の一過的なグルタミン酸の取込みが宿主細胞への侵入に必須である (2009)
  • Author(s): 高橋英之、渡邉治雄
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 神奈川県横浜市西区パシフィコ横浜
  • Year and Date: 2009-03-27
• [Presentation] 細菌感染時の髄膜炎菌の一過的なグルタミン酸の取込みが宿主細胞への侵入に必須である (2009)
  • Author(s): 高橋英之、渡邉治雄
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-24
• [Book] 感染症辞典 (2012)
  • Author(s): 川端寛樹、高橋英之
  • Publisher: オーム社
• [Book] 感染症辞典3-27ペスト (2012)
  • Author(s): 川端寛樹、高橋英之
  • Total Pages: 5
  • Publisher: オーム社
• [Book] 病原菌の今日的意味14.髄膜炎菌 (2011)
  • Author(s): 高橋英之、大西真
  • Publisher: 医薬ジャーナル社