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Novel analysis method of hematopoiesis applying to embryonic lethal mice

Research Project

Project/Area Number 21790480
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionSt.Marianna University School of Medicine

Principal Investigator

WADA Haruka  St.Marianna University School of Medicine, 医学(系)研究科(研究院), 講師 (70392181)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsiPS細胞 / T細胞 / B細胞 / 造血前駆細胞 / 分化誘導 / リプログラミング / 血液細胞分化 / T細胞分化 / B細胞分化
Research Abstract

Forced expression of certain transcription factors in somatic cells results in generation of induced pluripotent stem (iPS) cells, which differentiate into various cell types. We investigated T-cell and B-cell lineage differentiation from iPS cells in vitro. iPS cells differentiated into lymphocytes in OP9 co-culture systems. iPS cells efficiently differentiated into T-cell lineage. However, iPS cells including B-iPS cells were relatively resistant to B-cell lineage differentiation. One of the reasons of the failure of B-cell lineage differentiation seemed due to a defect of Pax5 expression in the differentiated cells. Therefore, current in vitro differentiation systems using iPS cells are sufficient for inducing T-cell but not B-cell lineage.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (25 results)

All 2011 2010 2009 Other

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (11 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] Successful differentiation to T cells, but unsuccessful B cell generation, from B-cell-derived induced pluripotent stem cells2011

    • Author(s)
      Wada H, Kojo S, Kusama C, Okamoto N, Sato Y, Ishizuka B, Seino K
    • Journal Title

      Int Immunol. 23

      Pages: 65-74

    • NAID

      10030575944

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinical Significance of Side Population in Ovarian Cancer Cells.2011

    • Author(s)
      Shinji H, Yoichi K, Kojo S, Wada H, Seino K, Kiguchi K, Ishizuka B.
    • Journal Title

      Human cell 24

      Pages: 9-12

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Side population is increased in paclitaxel-resistant ovarian cancer cell lines regardless of resistance to cisplatin.2011

    • Author(s)
      Kobayashi Y, Seino KI, Hosonuma S, Ohara T, Itamochi H, Isonishi S, Kita T, Wada H, Kojo S, Kiguchi K.
    • Journal Title

      Gynecol Oncol. 121

      Pages: 390-394

    • Related Report
      2010 Final Research Report
  • [Journal Article] Successful differentiation to T cells, but unsuccessful B cell generation, from B-cell-derived induced pluripotent stem cells2011

    • Author(s)
      Wada H, et al.
    • Journal Title

      Int Immunol

      Volume: 23 Pages: 65-74

    • NAID

      10030575944

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinical Significance of Side Population in Ovarian Cancer Cells2011

    • Author(s)
      Hosonuma S, et al.
    • Journal Title

      Human cell

      Volume: 24 Pages: 9-12

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Combining carbon ion radiotherapy and local injection of α-galactosylceramide-pulsed dendritic cells inhibits lung metastases in an in vivo murine model.2011

    • Author(s)
      Ohkubo Y, et al.
    • Journal Title

      International Journal of Radiation Oncology * Biology * Physics.

      Volume: 78 Pages: 1524-31

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Side population is increased in paclitaxel-resistant ovarian cancer cell lines regardless of resistance to cisplatin.2011

    • Author(s)
      Kobayashi Y, et al.
    • Journal Title

      Gynecol Oncol.

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Combining Carbon Ion Radiotherapy and Local Injection of α-Galactosylceramide-Pulsed Dendritic Cells Inhibits Lung Metastases in an In Vivo Murine Model2010

    • Author(s)
      Ohkubo Y, Iwakawa M, Seino K, Nakawatari M, Wada H, Kamijuku H, Nakamura E, Nakano T, Imai T
    • Journal Title

      International Journal of Radiation Oncology * Biology * Physics 78

      Pages: 1524-1531

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of an improved mouse model for infantile neuroaxonal dystrophy that shows early disease onset and bears a point mutation in Pla2g62009

    • Author(s)
      Wada H, Yasuda T, Miura I, Watabe K, Sawa C, Kamijuku H, Kojo S, Taniguchi M, Nishino I, Wakana S, Yoshida H, Seino K.
    • Journal Title

      Am J Pathol. 175

      Pages: 2257-2263

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] B細胞のiPS細胞化とB-iPS細胞からのT細胞への分化誘導2010

    • Author(s)
      和田はるか, 他
    • Organizer
      第9回再生医療学会総会
    • Place of Presentation
      広島国際会議場(広島県)
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] In vitro T or B lineage differentiation from mouse splenic B cell-derived induced pluripotent stem cells2010

    • Author(s)
      Wada H., et al.
    • Organizer
      ISSCR 8th Annual Meeting
    • Place of Presentation
      San Fransisco
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] In vitro T or B lineage differentiation from mouse splenic B cell-derived induced pluripotent stem cells2010

    • Author(s)
      Wada H
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe
    • Related Report
      2010 Annual Research Report
  • [Presentation] In vitro T, B or NK cell lineage differentiation from mouse splenic B cell-derived induced pluripotent stem cells.2010

    • Author(s)
      Wada H
    • Organizer
      12th Meeting of the Society for Natural Immunity & NK2010
    • Place of Presentation
      Croatia
    • Related Report
      2010 Annual Research Report
  • [Presentation] Generation of T cells from iPS cells and their anti-tumor effect in vivo2010

    • Author(s)
      Wada H
    • Organizer
      The Second Workshop of Synthetic Immunology
    • Place of Presentation
      Kyoto
    • Related Report
      2010 Annual Research Report
  • [Presentation] iPS細胞およびES細胞からの機能的T細胞の作製2009

    • Author(s)
      和田はるか, 他
    • Organizer
      第39回日本免疫学会
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Year and Date
      2009-12-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多機能幹細胞からの機能的T細胞の生成2009

    • Author(s)
      和田はるか, 他
    • Organizer
      第45回日本移植学会総会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細胞リプログラミング技術を用いた新規免疫細胞療法の開発2009

    • Author(s)
      和田はるか
    • Organizer
      聖マリアンナ医科大学医学会第57回学術集会
    • Place of Presentation
      聖マリアンナ医科大学(神奈川県)
    • Year and Date
      2009-07-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Generation of functional T cells from ES cells and induced pluripotent stem(iPS) cells in vitro2009

    • Author(s)
      和田はるか, 他
    • Organizer
      第13回日本がん免疫学会
    • Place of Presentation
      北九州国際会議場(福岡県)
    • Year and Date
      2009-06-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Generation of functional T cells from ES ceUs and induced pluripotent stem (iPS) cells in vitro2009

    • Author(s)
      Haruka Wada, et, al
    • Organizer
      The 5th International Workshop of Kyoto T Cell Conference
    • Place of Presentation
      京都大学芝蘭会館(京都府)
    • Year and Date
      2009-06-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] Generation of functional T cells from ES cells and induced pluripotent stem (iPS) cells in vitra2009

    • Author(s)
      Haruka Wada, et. al
    • Organizer
      The 7th Stem Cell Research Symposium Program
    • Place of Presentation
      泉ガーテ"ンキ"ヤラリー(東京都)
    • Year and Date
      2009-05-16
    • Related Report
      2009 Annual Research Report
  • [Book] NotchシグナルによるNK細胞の分化と機能制御、臨床免疫・アレルギー科2010

    • Author(s)
      和田はるか、清野研一郎
    • Related Report
      2010 Final Research Report
  • [Book] インフルエンザ感染におけるCTLA4-1gの効果2010

    • Author(s)
      神宿元, 他
    • Publisher
      科学評論社
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.igm.hokudai.ac.jp/byo-ri/about1.html

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] PRODUCTION METHOD OF IMMUNE CELLS2010

    • Inventor(s)
      清野研一郎、和田はるか
    • Industrial Property Rights Holder
      聖マリアンナ医科大学
    • Filing Date
      2010-07-28
    • Related Report
      2010 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] PRODUCTION METHOD OF IMMUNE CELLS2010

    • Inventor(s)
      清野研一郎、和田はるか
    • Industrial Property Rights Holder
      聖マリアンナ医科大学
    • Filing Date
      2010-07-28
    • Related Report
      2010 Annual Research Report
    • Overseas

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Published: 2009-04-01   Modified: 2016-04-21  

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