|Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
I tried the establishment of the effective cell culture method of regulatory T cells. To clarify the role of selectins/selectin-ligands in the cell proliferation and the function of Treg cells, we examined the cell proliferation rate and the function of Treg cells, using P-selectin-/-mice and PSGL-1-/-mice. The growth rate of P-selectin-/-Treg cells and PSGL-1-/-Treg cells were high in comparison with the wild-type Treg cells. PSGL-1-/-Treg cells could inhibit wild-type T cell proliferation in in-vitro suppression assay.