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Glycan ligands of killer lectin-like receptors and regulation of NK functions

Research Project

Project/Area Number 21790544
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionToho University

Principal Investigator

HIGAI Koji  Toho University, 薬学部, 講師 (70297711)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsNK細胞 / Killer LRs / NCRs / sialyl Lewis X / sialyl lewis X / heparin / NKG2D / NCR / 糖鎖リガンド / CD94 / Lectin / Polysaccharides
Research Abstract

Using recombinant extracellular lectin-like domains of KLRs (rKLRs) and NCRs (rNCRs) on NK cells, we evaluated their ligands specificity and binding affinities. Our data resulted that rKLRs and rNCRs can bind to sialyl Lewis X, GAGs and sulfated polysaccharides.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (46 results)

All 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (32 results) Book (3 results)

  • [Journal Article] Binding of Natural cytotoxicity receptor NKp46 to sulfate- and α2,3-NeuAc-containing glycans and its mutagenesis.2011

    • Author(s)
      Ito K., Higai K., Sakurai M., Shinoda C., Yanai K., Azuma Y., Matsumoto K.
    • Journal Title

      Biochem, Biophys.Res.Commun. 406(3)

      Pages: 377-82

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] NKG2A and NKG2C bind to sulfated glycans and α2,3-NeuAc- containing glycoproteins.2011

    • Author(s)
      Xin X, Higai K., Imaizumi Y., Suzuki C, Ito K, Itoh A., Matsumoto S., Azuma Y, Matsumoto K
    • Journal Title

      Biol.Pharm.Bull. 34(4)

      Pages: 480-485

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] NKG2A and NKG2C bind to sulfated glycans and α2,3-NeuAc-containing glycoproteins.2011

    • Author(s)
      Xin X., Hgai K., et al.
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 34(4) Pages: 480-485

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Binding of Natural cytotoxicity receptor NKp46 to sulfate- and α2,3-NeuAc-containing glycans and its mutagenesis2011

    • Author(s)
      Ito K., Higai K, et al.
    • Journal Title

      Biochem, Biophys.Res.Commun

      Volume: 406(3) Pages: 377-382

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Binding properties of CD94 to sulfated glycans and a2,3-NeuAc-containing glycoproteins and mutagenesis analysis2011

    • Author(s)
      Higai K, et al.
    • Journal Title

      The Open Biotechnology Journal

      Volume: (inpress)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Binding affinities of NKG2D and CD94 to sialyl Lewis X-expressing N-glycans and heparin.2010

    • Author(s)
      Higai K., Suzuki C., Imaizumi Y., Xin Xin, Azuma Y., Matsumoto K
    • Journal Title

      Biol.Pharm.Bull. 34(1)

      Pages: 8-12

    • NAID

      130000402255

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Binding affinities of NKG2D and CD94 to sialyl Lewis X-expressing N-glycans and heparin2010

    • Author(s)
      Higai K, et al.
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 34(1) Pages: 8-12

    • NAID

      130000402255

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NKG2D and CD94 bind to heparin and sulfate-containing polysaccharides.2009

    • Author(s)
      Higai K., Imaizumi Y., Suzuki C, Azuma Y., Matsumoto K.
    • Journal Title

      Biochem, Biophys.Res.Commun. 386

      Pages: 709-714

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] NKG2D and CD94 bind to multimeric α2,3-linked N-acetylneu raminic acid.2009

    • Author(s)
      Imaizumi Y., Higai K., Suzuki C, Azuma Y, Matsumoto K.
    • Journal Title

      Biochem, Biophys.Res.Commun. 382

      Pages: 604-608

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] NKG2D and CD94 bind to heparin and sulfate-containing polysaccharides2009

    • Author(s)
      K.Higai, et al.
    • Journal Title

      Biochem,Biophys.Res.Commun 7174

      Pages: 709-714

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Binding properties of CD94 to sulfated glycans and α2,3-NeuAc-containing glycoprotein and its mutagenesis analysis.

    • Author(s)
      Higai K., Itoh A., Imaizumi Y., Suzuki C., Xin X., Azuma Y., Matsumoto K.
    • Journal Title

      The Open Biotechnology Journal. in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] Killer Lectin ReceptorおよびNatural Cytotoxicity Receptorの糖鎖リガンド特異性2011

    • Author(s)
      桧貝孝慈、松本宏治郎
    • Organizer
      一般シンポジウム「糖鎖発現・機能の異常と疾患」第131回日本薬学会年会,震災により
    • Place of Presentation
      DVD・冊子による発表
    • Year and Date
      2011-03-31
    • Related Report
      2010 Final Research Report
  • [Presentation] Killer Lectin ReceptorおよびNatural Cytotoxicity Receptorの糖鎖リガンド特異性2011

    • Author(s)
      桧貝孝慈、松本宏治郎
    • Organizer
      第131回日本薬学会年会
    • Place of Presentation
      (震災により、DVD・冊子による発表)
    • Year and Date
      2011-03-31
    • Related Report
      2010 Annual Research Report
  • [Presentation] NCR2およびNCR3の糖鎖リガンドの解析2011

    • Author(s)
      篠田千尋、伊藤健一郎、桧貝孝慈、東祐太郎、松本宏治郎
    • Organizer
      第131回日本薬学会年会,震災により
    • Place of Presentation
      DVD・冊子による発表
    • Year and Date
      2011-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] NCR1の糖鎖リガンドの解析2011

    • Author(s)
      松本宏治郎、櫻井瑞葉、伊藤健一郎、桧貝孝慈、柳内和幸、東祐太郎
    • Organizer
      第131回日本薬学会年会,震災により
    • Place of Presentation
      DVD・冊子による発表
    • Year and Date
      2011-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2DおよびCD94のsialyl Lewis X発現N型糖鎖およびヘパリンへの結合親和性2011

    • Author(s)
      桧貝孝慈、鈴木千穂、今泉雄三、Xin Xin、東祐太郎、松本宏治郎
    • Organizer
      第131回日本薬学会年会,震災により
    • Place of Presentation
      DVD・冊子による発表
    • Year and Date
      2011-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2AおよびCの多分岐シアル酸およびヘパリンへの結合2011

    • Author(s)
      Xin Xin、桧貝孝慈、東祐太郎、松本宏治郎
    • Organizer
      第131回日本薬学会年会,震災により
    • Place of Presentation
      DVD・冊子による発表
    • Year and Date
      2011-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2AおよびCの多分岐シアル酸およびヘパリンへの結合2011

    • Author(s)
      Xin Xin、桧貝孝慈
    • Organizer
      第131回日本薬学会年会
    • Place of Presentation
      (震災により、DVD・冊子による発表)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] NKG2DおよびCD94のsialyl Lewis X発現N型糖鎖およびヘパリンへの結合親和性2011

    • Author(s)
      桧貝孝慈
    • Organizer
      第131回日本薬学会年会
    • Place of Presentation
      (震災により、DVD・冊子による発表)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] NCR1の糖鎖リガンドの解析2011

    • Author(s)
      松本宏治郎、櫻井瑞葉、伊藤健一郎、桧貝孝慈
    • Organizer
      第131回日本薬学会年会
    • Place of Presentation
      (震災により、DVD・冊子による発表)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] NCR2およびNCR3の糖鎖リガンドの解析2011

    • Author(s)
      篠田千尋、伊藤健一郎、桧貝孝慈
    • Organizer
      第131回日本薬学会年会
    • Place of Presentation
      (震災により、DVD・冊子による発表)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] NKG2AおよびCの多分岐シアル酸およびヘパリンへの結合2010

    • Author(s)
      Xin Xin、桧貝孝慈、東祐太郎、松本宏治郎
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2Dの多分岐シアル酸、GAGおよび硫酸化多糖類への結合2010

    • Author(s)
      松本早代、木村恵、今泉雄三、桧貝孝慈、柳内和幸、東祐太郎、松本宏治郎
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] CD94の多分岐シアル酸、GAGおよび硫酸化多糖類への結合2010

    • Author(s)
      伊藤あゆみ、今泉雄三、桧貝孝慈、東祐太郎、松本宏治郎
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] NCR2およびNCR3の糖鎖リガンドの解析2010

    • Author(s)
      松本宏治郎、伊藤健一郎、篠田千尋、桧貝孝慈、東祐太郎
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] NCR1の糖鎖リガンドの解析2010

    • Author(s)
      櫻井瑞葉、伊藤健一郎、桧貝孝慈、柳内和幸、東祐太郎、松本宏治郎
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] NCR1の糖鎖リガンドの解析2010

    • Author(s)
      櫻井瑞葉、伊藤健一郎、桧貝孝慈
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] NCR2およびNCR3の糖鎖リガンドの解析2010

    • Author(s)
      松本宏治郎、伊藤健一郎、篠田千尋、桧貝孝慈
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] CD94の多分岐シアル酸、GAGおよび硫酸化多糖類への結合2010

    • Author(s)
      伊藤あゆみ、今泉雄三、桧貝孝慈
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] NKG2Dの多分岐シアル酸、GAGおよび硫酸化多糖類への結合2010

    • Author(s)
      松本早代、木村恵、今泉雄三、桧貝孝慈
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] NKG2AおよびCの多分岐シアル酸およびヘパリンへの結合2010

    • Author(s)
      Xin Xin、桧貝孝慈
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] NKG2D and CD94 bind to multimeric α2,3-linked N-acetylneu raminic acids2010

    • Author(s)
      Imaizumi Y, Higai K, Suzuki C, Azuma Y, Matsumoto K
    • Organizer
      The 25th International Carbohydrate Symposium
    • Place of Presentation
      Makuhari, Japan
    • Year and Date
      2010-08-03
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2D and CD94 bind to heparin and sulfate-containing polysaccharides2010

    • Author(s)
      Higai K, Imaizumi Y, Suzuki C, Azuma Y, Matsumoto K
    • Organizer
      The 25th International Carbohydrate Symposium
    • Place of Presentation
      Makuhari, Japan
    • Year and Date
      2010-08-03
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2D and CD94 bind to heparin and sulfate-containing polysaccharides2010

    • Author(s)
      Higai K
    • Organizer
      The 25^<th> International Carbohydrate Symposium
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2010-08-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] NKG2D and CD94 bind to multimeric α2,3-linked N-acetylneuraminic acids2010

    • Author(s)
      Imaizumi Y, Higai K
    • Organizer
      The 25^<th> International Carbohydrate Symposium
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2010-08-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Natural cytotoxicity receptors のsialyl Lewis Xとの結合性2010

    • Author(s)
      桧貝孝慈, 伊藤健一郎, 東祐太郎, 松本宏次郎
    • Organizer
      第130回日本薬学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-31
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2D and CD94 bind to multimeric α2,3-linked N-acetylneu raminic acid.2009

    • Author(s)
      桧貝孝慈、今泉雄三、鈴木千穂、東祐太郎、松本宏治郎
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-24
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2D and CD94 bind to heparin and sulfate-containing polysaccharides.2009

    • Author(s)
      松本宏治郎、鈴木千穂、今泉雄三、桧貝孝慈、東祐太郎
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-24
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2A and NKG2C bind to multimeric N-acetylneuraminic acid and heparin.2009

    • Author(s)
      Xin Xin, 鈴木千穂、桧貝孝慈、東祐太郎、松本宏治郎
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-24
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2D and CD94 bind to heparin and sulfate-containing polysaccharides.2009

    • Author(s)
      K.Matsumoto, et al
    • Organizer
      第81回日本生化学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Glycan ligands of NKG2A and NKG2C.2009

    • Author(s)
      Xin X, Suzuki C, Higai K, Azuma Y, Matsumoto K
    • Organizer
      The 6th Seminar on Biomedical Sciences.
    • Place of Presentation
      Tokyo.
    • Year and Date
      2009-10-12
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2DおよびCD94の多分岐α2,3-シアル酸への結合2009

    • Author(s)
      鈴木千穂、今泉雄三、桧貝孝慈、東祐太郎、松本宏治郎
    • Organizer
      第29回日本糖質学会年会
    • Place of Presentation
      飛騨
    • Year and Date
      2009-09-10
    • Related Report
      2010 Final Research Report
  • [Presentation] NKG2DおよびCD94の多分岐alpha2,3-シアル酸への結合2009

    • Author(s)
      C.Suzuki, et al
    • Organizer
      第29回日本糖質学会年会
    • Place of Presentation
      飛騨世界生活文化センター
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Book] 「5.5組換え蛋白質:組換え蛋白質の発現と糖鎖修飾」「バイオマテリアルの基礎」分担2010

    • Author(s)
      桧貝孝慈、松本宏治郎
    • Publisher
      日本医学館
    • Related Report
      2010 Final Research Report
  • [Book] 「5.5 組換え蛋白質:組換え蛋白質の発現と糖鎖修飾」「バイオマテリアルの基礎」分担2010

    • Author(s)
      桧貝孝慈、松本宏次郎
    • Total Pages
      259
    • Publisher
      日本医学館
    • Related Report
      2010 Annual Research Report
  • [Book] 「組換え蛋白質:組換え蛋白質の発現と糖鎖修飾」バイオマテリアル-生体材料-特集「異分野融合のためのバイオマテリアルの基礎PART6」Vol.272009

    • Author(s)
      桧貝孝慈、松本宏治郎
    • Publisher
      日本医学館
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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