Analysis of denaturated protein in the central nervous system and endocrine system of drug abusers

• Project/Area Number: 21790612
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Legal medicine
• Research Institution: Osaka City University
• Principal Investigator: ISHIKAWA Takaki 大阪市立大学, 大学院・医学研究科, 准教授 (50381984)
• Research Collaborator: HITOSHI Maeda 大阪市立大学, 大学院・医学研究科, 教授 (20135049) ; TOMOMI Michiue 大阪市立大学, 大学院・医学研究科, 講師 (00529240) ; MASAFUMI Tomita 川崎医科大学, 准教授 (50113197)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 社会医学 / 細胞 / 組織 / ストレス / 蛋白質 / 薬物乱用 / 細胞・組織

Research Abstract

This study analyzed the expression dynamics of apoliprotein(Apo) E4, ApoB, and ApoJ, which are known to be increased in cases with neurodegenerative diseases such as Alzheimer's disease and Creutzfeldt-Jakob disease, in the central nervous system and endocrine system of metamphetamine and psychotropic drug abusers using immunohistochemical and molecular biological(mRNA expression) techniques. Although Apo expression showed site-to-site differences, characteristic expression patterns were detected not only in cases exposed to extreme temperature conditions in traumatic events, such as fire fatality, hyperthermia, or hypothermia, but also in drug abusers.

Research Products

• [Journal Article] Evaluation of postmortem serum and cerebrospinal fluid growth hormone levels in relation to the cause of death in forensic autopsy (2011)
  • Author(s): Ishikawa T, Michiue T, Maeda H
  • Journal Title: Hum Cell Volume: 24 Issue: 2 Pages: 74-77
  • DOI: 10.1007/s13577-011-0012-5
  • Peer Reviewed
• [Journal Article] Postmortem biochemistry and immunohistochemistry of chromogranin A as a stress marker with special regard to fatal hypothermia and hyperthermia (2011)
  • Author(s): Yoshida C, Ishikawa T, Michiue T, Quan L, Maeda H
  • Journal Title: Int J Legal Med Volume: 125 Issue: 1 Pages: 11-20
  • DOI: 10.1007/s00414-009-0374-3
  • Peer Reviewed
• [Journal Article] Postmortem serotonin levels in cerebrospinal and pericardial fluids with regard to the cause of death in medicolegal autopsy (2011)
  • Author(s): Quan L, Ishikawa T, Hara J, Michiue T, Chen JH, Wang Q, Zhu BL, Maeda H
  • Journal Title: Leg Med Volume: 13 Issue: 2 Pages: 75-78
  • DOI: 10.1016/j.legalmed.2010.11.003
  • Peer Reviewed
• [Journal Article] Water-restraint stress enhances methamphetamine-induced cardiotoxicity (2011)
  • Author(s): Tomita M, Katsuyama H, Watanabe Y, Hidaka K, Yoshitome K, Miyaishi S, Ishikawa T, Shinone K
  • Journal Title: Chemico-Biol Interact Volume: 190 Issue: 1 Pages: 54-61
  • DOI: 10.1016/j.cbi.2011.01.025
  • Peer Reviewed
• [Journal Article] Forensic biochemistry for functional investigation of death : Concept and practical application (2011)
  • Author(s): Maeda H, Ishikawa T, Michiue T
  • Journal Title: Leg Med Volume: 13 Issue: 2 Pages: 55-67
  • DOI: 10.1016/j.legalmed.2010.12.005
  • Peer Reviewed
• [Journal Article] Perdekamp MG, Pollak S, Maeda H, Immunohistochemistry of catecholamines in the hypothalamic-pituitary-adrenal system with special regard to fatal hypothermia and hyperthermia (2011)
  • Author(s): Ishikawa T, Yoshida C, Michiue T
  • Journal Title: Leg Med Volume: 12 Issue: 3 Pages: 121-127
  • DOI: 10.1016/j.legalmed.2010.01.004
  • Peer Reviewed
• [Journal Article] Evaluation of postmortem serum and cerebrospinal fluid growth hormone levels in relation to the cause of death in forensic autopsy. (2011)
  • Author(s): Ishikawa T, Michiue T, Maeda H.
  • Journal Title: Hum Cell Volume: (In press)
  • Peer Reviewed
• [Journal Article] Evaluation of human brain damage in fire fatality by quantification of basic fibroblast growth factor (bFGF), glial fibrillary acidic protein (GFAP) and single-stranded DNA (ssDNA) immunoreactivities. (2011)
  • Author(s): Wang Q, Ishikawa T, Michiue T, Zhu B-L, Maeda H.
  • Journal Title: Forensic Science International Volume: (In press)
  • Peer Reviewed
• [Journal Article] Combined analyses of creatine kinase MB, cardiac troponin I and myoglobin in pericardial and cerebrospinal fluids to investigate myocardial and skeletal muscle injury in medicolegal autopsy cases. (2011)
  • Author(s): Wang Q, Ishikawa T, Michiue T, Zhu B-L, Maeda H.
  • Journal Title: Legal Medicine Volume: (In press)
  • Peer Reviewed
• [Journal Article] Immunohistochemistry of catecholamines in the hypothalamic-pituitary-adrenal system with special regard to fatal hypothermia and hyperthermia. (2010)
  • Author(s): Ishikawa T, Yoshida C, Michiue T, Perdekamp MG, Pollak S, Maeda H.
  • Journal Title: Legal Medicine Volume: 12 Pages: 121-127
  • NAID: 10027575793
  • Peer Reviewed
• [Journal Article] Water-restraint stress enhances methamphetamine-induced cardiotoxicity. (2010)
  • Author(s): Tomita M, Katsuyama H, Watanabe Y, Hidaka K, Yoshitome K, Miyaishi S, Ishikawa T, Shinone K.
  • Journal Title: Chemico-Biological Interact Volume: (In press)
  • Peer Reviewed
• [Journal Article] Postmortem serotonin levels in cerebrospinal and pericardial fluids with regard to the cause of death in medicolegal autopsy. (2010)
  • Author(s): Quan L, Ishikawa T, Hara J, Michiue T, Chen JH, Wang Qi, Zhu BL, Maeda H.
  • Journal Title: Legal Medicine Volume: 13 Pages: 75-78
  • Peer Reviewed
• [Journal Article] Immunohistochemistry of catecholamines in the hypothalamic-pituitary-adrenal system with special regard to fatal hypothermia and hyperthermia. (2010)
  • Author(s): Ishikawa T, Yoshida C, Michiue T, Perdekamp MG, Pollak S, Maeda H.
  • Journal Title: Legal Medicine (In press)
  • NAID: 10027575793
• [Journal Article] Postmortem biochemistry and immunohistochemistry of chromogranin Aas a stress marker with special regard to fatal hypothermia and hypert hermia. (2009)
  • Author(s): Yoshida C, Lshikawa T, Michiue T, Quan L, Maeda H.
  • Journal Title: International Journal of Legal Medicine (In press)
• [Journal Article] Histopathological changes of the hippocampus neurons in brain injury. (2009)
  • Author(s): Li DR,Ishikawa T, Zhao D, Michiue T, Quan L, Zhu BL, Maeda H.
  • Journal Title: Histology and Histopathology 24 Pages: 1113-1120
• [Presentation] 剖検例における急性薬物中毒死の生化学指標 (2011)
  • Author(s): 宮里公子, 石川隆紀, 道上知美, 織谷茂樹, 王起, 小川雅史, 前田均
  • Organizer: 第31回日本中毒学会西日本部会
  • Place of Presentation: 大阪,りんくう総合医療センター,市立泉佐野病院
  • Year and Date: 2011-02-26
• [Presentation] Molecular pathology of apolipoprotein E4 in human brain with regard to traumatic stress (2011)
  • Author(s): Ishikawa T, Michiue T, Maeda H
  • Organizer: 8^<th> International Symposium on Advances in Legal Medicine
  • Place of Presentation: Johann Wolfgang Goethe-University, Frankfurt, Germany
• [Presentation] Molecular pathological approaches to pathophysiology of death in routine forensic casework : present observations and outlook (2011)
  • Author(s): Ishikawa T
  • Organizer: 8^<th> International Symposium on Advances in Legal Medicine
  • Place of Presentation: Johann Wolfgang Goethe-University, Frankfurt, Germany
• [Presentation] Post-mortem urinary catecholamine levels with regard to the cause of death (2011)
  • Author(s): Hara J, Ishikawa T, Michiue T, Maeda H
  • Organizer: 8^<th> International Symposium on Advances in Legal Medicine
  • Place of Presentation: Johann Wolfgang Goethe-University, Frankfurt, Germany
• [Presentation] Molecular pathology of pulmonary surfactants and cytokines in the lung with special regard to fatal intoxication, hypothermia and hyperthermia (2011)
  • Author(s): Ishikawa T, Miyazato T, Michiue T, Maeda H, Miyazato T, Michiue T, Maeda H
  • Organizer: 8^<th> International Symposium on Advanced in Legal Medicine
  • Place of Presentation: Johann Wolfgang Goethe-University, Frankfurt, Germany
• [Presentation] Different death processes of fire fatality as demonstrated by molecular pathology of pulmonary surfactants and cytokines with special regard to contribution of burns and carbon monoxide intoxication (2011)
  • Author(s): Ishikawa T, Miyazato T, Michiue T, Maeda H
  • Organizer: 8^<th> International Symposium on Advanced in Legal Medicine
  • Place of Presentation: Johann Wolfgang Goethe-University, Frankfurt, Germany
• [Presentation] Molecular pathology of pulmonary surfactants and cytokines in drowning (2011)
  • Author(s): Miyazato T, Ishikawa T, Michiue T, Maeda H
  • Organizer: 第95次日本法医学会学術全国集会
  • Place of Presentation: 福島,コラッセ福島
• [Presentation] Molecular pathology of apolipoprotein E4 in human brain with regard to traumatic stress (2011)
  • Author(s): Ishikawa T, Michiue T, Maeda H
  • Organizer: 8^<th> International Symposium on Advances in Legal Medicine
  • Place of Presentation: Johann Wolfgang Goethe-University, Frankfurt, Germay
• [Presentation] Molecular pathological approaches to pathophysiology of death in routine forensic casework : present observations and outlook (2011)
  • Author(s): Ishikawa T
  • Organizer: 8^<th> International Symposium on Advances in Legal Medicine
  • Place of Presentation: Johann Wolfgang Goethe-University, Frankfurt, Germay(招聘講演)
• [Presentation] Molecular pathology of pulmonary surfactants and cutokines in the lung with special regard to fatal intoxication, hypothermia and hyperthermia (2011)
  • Author(s): Ishikawa T, Miyazato T, Michiue T, Maeda H
  • Organizer: 8^<th> International Symposium on Advances in Legal Medicine
  • Place of Presentation: Johann Wolfgang Goethe-University, Frankfurt, Germay
• [Presentation] Different death processes of fire fatality as demonstrated by molecular pathology of pulmonary surfactants and cytokines with special regard to contribution of burns and carbon monoxide intoxication (2011)
  • Author(s): Ishikawa T, Miyazato T, Michiue T, Maeda H
  • Organizer: 8^<th> International Symposium on Advances in Legal Medicine
  • Place of Presentation: Johann Wolfgang Goethe-University, Frankfurt, Germay
• [Presentation] Post-mortem urinary catecholamine levels with regard to the cause of death (2011)
  • Author(s): Hara J, Ishikawa T, Michiue T, Maeda H
  • Organizer: 8^<th> International Symposium on Advances in Legal Medicine
  • Place of Presentation: Johann Wolfgang Goethe-University, Frankfurt, Germay
• [Presentation] Molecular pathology of pulmonary surfactants and cyokines in drowning (2011)
  • Author(s): Miyazato T, Ishikawa T, Michiue T, MaedaH
  • Organizer: 第95次日本法医学会学術全国集会
  • Place of Presentation: 福島,コラッセ福島
• [Presentation] Postmortem biochemistry and immunohistochemistry ofβ-endorphin with regard to the cause of death in forensic autopsy (2010)
  • Author(s): Ishikawa T, Michiue T, Wang Q, Chen JH, Yoshida C, Miyazato T, Maeda H
  • Organizer: The 10^<th> Indo-Pacific Congress on Legal Medicine and Forensic Science
  • Place of Presentation: Noida, India
  • Year and Date: 2010-10-25
• [Presentation] Postmortem biochemistry and immunohistochemistry of β-endorphin with regard to the cause of death in forensic autopsy. (2010)
  • Author(s): Ishikawa T, Michiue M, Wang Q, Chen J-H, Yoshida C, Miyazato T, Maeda H.
  • Organizer: The 10th Indo-Pacific Congress on Legal Medicine and Forensic Science
  • Place of Presentation: Noida, India
  • Year and Date: 2010-10-25
• [Presentation] Immunohistochemistry of apolipoproteins in the hypothalamic-pituitary-adrenal system with special regard to fatal poisoning in forensic autopsy (2010)
  • Author(s): Ishikawa T, Michiue T, Miyazato T, Perdekamp MG, Pollak S, Maeda H
  • Organizer: Jahrestagung der Deutschen Gesellschaft fur Rechtsmedizin
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2010-09-24
• [Presentation] Immunohistochemistry of apolipoproteins in the hypothalamic-pituitary-a drenal system with special regard to fatal poisoning in forensic autopsy. (2010)
  • Author(s): Ishikawa T, Michiue T, Miyazato T, Perdekamp M-G, Pollak S, Maeda H.
  • Organizer: 89. Jahrestagung der Deutschen Gesellschaft fur Rechtsmedizin
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2010-09-24
• [Presentation] Pulmonary molecular pathology of drowning. (2010)
  • Author(s): Miyazato T, Ishikawa T, Michiue T, Maeda H.
  • Organizer: 89. Jahrestagung der Deutschen Gesellschaft fur Rechtsmedizin
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2010-09-24
• [Book] Encyclopedia Forensic Science. Hyrperthermia and Hypothermia (2012)
  • Author(s): Ishikawa T, Maeda H
  • Publisher: ELSEVIA(in press)
• [Book] Encyclopedia Forensic Science. Systemic response to trauma (2012)
  • Author(s): Ishikawa T, Maeda H
  • Publisher: ELSEVTA(in press)
• [Book] Encyclopedia of Forensic Science
  • Author(s): Ishikawa T, Maeda H
  • Publisher: Hyperthermia and hypothermia(in press)
• [Book] Encyclopedia of Forensic Science
  • Author(s): Ishikawa T, Maeda H
  • Publisher: Systemic response to trauma(in press)