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Evaluation of toxicity of drugs based on drug distributions in organs by imaging mass spectrometry

Research Project

Project/Area Number 21790616
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionNational Research Institute of Police Science

Principal Investigator

KUWAYAMA Kenji  科学警察研究所, 法科学第三部, 研究員 (40356233)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords法中毒学 / イメージング質量分析 / 乱用薬物 / 体内分布 / 毒性 / メタンフェタミン / MDMA / 社会医学 / 薬学 / 分析科学
Research Abstract

Drugs(methamphetamine and 3, 4-methylenedioxymethamphetmaine) were administered to mice, and the effects of drugs on locomotor activity, body temperature and behavior were examined. Moreover, the organs(brain, liver and kidney) were removed from mice after drug administration and then drug distributions and drug concentrations in organs were measured by MALDI/imaging MS and LC/MS, respectively. Data obtained using the standardized method were integrated on a data sheet every drug. The increment of data sheets would be useful for the comparison of toxicity among drugs and the estimation of toxicity of novel drugs.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (8 results)

All 2012 2011 2010

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (5 results)

  • [Journal Article] Rapid, simple, and highly sensitive analysis of drugs in biological samples using thin-layer chromatography coupled with matrix-assisted laser desorption/ionization mass spectrometry2012

    • Author(s)
      Kenji Kuwayama, Kenji Tsujikawa, Hajime Miyaguchi, Tatsuyuki Kanamori, Yuko T. Iwata, Hiroyuki Inoue
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: 402 Issue: 3 Pages: 1257-1267

    • DOI

      10.1007/s00216-011-5576-0

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of 3, 4-methylenedioxymethamphetamine and methamphetamine during metabolism by in vitro human metabolic enzymes and in rats2011

    • Author(s)
      Kenji Kuwayama, Kenji Tsujikawa, Hajime Miyaguchi, Tatsuyuki Kanamori, Yuko T. Iwata, Hiroyuki Inoue
    • Journal Title

      Journal of Forensic Sciences

      Volume: (In press) Issue: 4 Pages: 1008-1013

    • DOI

      10.1111/j.1556-4029.2011.02039.x

    • Year and Date
      2011-12-28
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distribution measurement of amphetamine-type stimulants in organs using micropulverized extraction and liquid chromatography/tandem mass spectrometry to complement drug distribution using mass spectrometry imaging2011

    • Author(s)
      Kenji Kuwayama, Kenji Tsujikawa, Hajime Miyaguchi, Tatsuyuki Kanamori, Yuko T. Iwata, Hiroyuki Inoue
    • Journal Title

      Rapid Communications in Mass Spectrometry

      Volume: 25 Issue: 17 Pages: 2397-2406

    • DOI

      10.1002/rcm.5145

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Presentation] マトリックス支援レーザー脱離イオン化/イメージング質量分析による乱用薬物の臓器内分布測定の最適化2012

    • Author(s)
      桑山健次
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-03-31
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Development of automatic matrix spraying system with an air brush and a turn table for drug distribution measurement in organs by matrix-assisted laser desorption/ionization imaging mass spectrometry2012

    • Author(s)
      Kenji Kuwayama
    • Organizer
      PITTCON 2012
    • Place of Presentation
      オーランドオレンジカウンティコンベンションセンター(アメリカ)
    • Year and Date
      2012-03-13
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 薄層クロマトグラフィー/マトリックス支援レーザー脱離イオン化/イメージング質量分析による生体試料中の薬物の同定及び画像化2011

    • Author(s)
      桑山健次
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      ツインメッセ静岡(静岡市)
    • Year and Date
      2011-03-30
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Development of analytical method for distribution measurement of amphetamine-type stimulants in organs2010

    • Author(s)
      Kenji Kuwayama
    • Organizer
      PACIFICHEM 2010
    • Place of Presentation
      ハワイコンベンションセンター(アメリカ)
    • Year and Date
      2010-12-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of analytical method for distribution measurement of amphetamine-type stimulants in organs2010

    • Author(s)
      桑山健次
    • Organizer
      Pacifichem2010
    • Place of Presentation
      ハワイコンベンションセンター(アメリカ)
    • Year and Date
      2010-12-15
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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