Cooperation of TGF-β and Notch in the induction of intestinal metaplasia and cancer stem cells of the esophagus and stomach
| Project/Area Number |
21790647
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Tohoku University |
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Principal Investigator |
ASANO Naoki Tohoku University, 病院, 助教 (20526454)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 上部消化管学(食道、胃、十二指腸) / ヘリコバクター・ピロリ / TGF-β / 腸上皮化生 / NFκB / NFkB |
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| Research Abstract |
Immunohistochemistry of human gastric cancer specimens revealed that only poorly differentiated gastric cancer cells express the stem cell marker CD133. The same tendency was observed in gastric cancer cell lines. We established a CD133 expressing gastric cancer cell line KATOIII that stably express doxycycline inducible Notch1 siRNA vector. Analysis using this and other cell line revealed that CD133 is directly regulated by Notch1 through RBP-Jk. We also found out that Notch1 regulates proliferation and tumorigenesis in these cells.
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Report
(3 results)
Research Products
(6 results)
