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Analyses of cell-cell interaction regulating proliferation and differentiation of hepatic stem cells during early-liver development

Research Project

Project/Area Number 21790650
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionThe University of Tokyo

Principal Investigator

KAMIYA Akihide  The University of Tokyo, 医科学研究所, 助教 (30321904)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords肝幹細胞 / 肝再生 / 肝臓病学 / 肝細胞生物学 / 肝幹・前駆細胞 / 肝発生 / 細胞間相互作用
Research Abstract

Hepatic stem/progenitor cells (HSPCs) in the liver bud are postulated to migrate into septum transversum mesenchyme at around embryonic day (E) 9 in mice. Mid-fetal livers (E11.5-E14.5) support proliferation and differentiation of hematopoietic stem cells derived from the aorta-gonad-mesonephros region. We established a new co-culture system, using mouse embryonic fibroblast cells, to permit analysis of the phenotypes of early-fetal HSPC candidates. In this co-culture system, CD13^+Dlk^+ cells purified from mouse early-fetal livers (E9.5 and E10.5) formed colonies composed of both albumin-positive hepatocytic cells and cytokeratin (CK) 19-positive cholangiocytic cells, indicating that early-fetal CD13^+Dlk^+ cells have properties of bi-potent progenitor cells. These first prospective studies of early-fetal HSPC candidates demonstrate that bi-potent stem/progenitor cells exist before E11.5.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (19 results)

All 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 2 results) Presentation (5 results) Book (1 results) Remarks (4 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 3 results)

  • [Journal Article] Bmi1 promotes hepatic stem cell expansion and tumorigenicity in both Ink4a/Arf-dependent and independent manners.2010

    • Author(s)
      Chiba T, Seki A, Aoki R, Ichikawa H, Negishi M, Miyagi S, Oguro H, Saraya A, Kamiya A, Nakauchi H, Yokosuka O, Iwama A.
    • Journal Title

      Hepatology 52

      Pages: 1111-1123

    • Related Report
      2010 Final Research Report
  • [Journal Article] Bmi1 promotes hepatic stem cell expansion and tumorigenicity in both Ink4a/Arf-dependent and independent manners.2010

    • Author(s)
      Chiba T, Seki A, Aoki R, Ichikawa H, Negishi M, Miyagi S, Oguro H, Saraya A, Kamiya A, Nakauchi H, Yokosuka O, Iwama A.
    • Journal Title

      Hepatology

      Volume: 52 Pages: 1111-1123

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enrichment and clonal culture of progenitor cells during mouse postnatal liver development in mice.2009

    • Author(s)
      Kamiya A, Kakinuma S, Yamazaki Y, Nakauchi H.
    • Journal Title

      Gastroenterology 137

      Pages: 1114-1126

    • Related Report
      2010 Final Research Report
  • [Journal Article] Cancer stem cells in hepatocellular carcinoma : Recent progress and perspective.2009

    • Author(s)
      Chiba T, Kamiya A, Yokosuka O, Iwama A.
    • Journal Title

      Cancer Letter 286

      Pages: 145-153

    • Related Report
      2010 Final Research Report
  • [Journal Article] Enrichment and clonal culture of progenitor cells during mouse postnatal liver development in mice2009

    • Author(s)
      Kamiya A, Kakinuma S, Yamazaki Y, Nakauchi H.
    • Journal Title

      Gastroenterology 137

      Pages: 1114-1126

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cancer stem cells in hepatocellular carcinoma : Recent progress and perspective2009

    • Author(s)
      Chiba T, Kamiya A, Yokosuka O, Iwama A.
    • Journal Title

      Cancer Letter 286

      Pages: 145-153

    • Related Report
      2009 Annual Research Report
  • [Presentation] 肝発生初期および中期由来の肝幹/前駆細胞のin vitro増幅系の構築2010

    • Author(s)
      紙谷聡英、中内啓光
    • Organizer
      第14回日本肝臓学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-10-14
    • Related Report
      2010 Final Research Report
  • [Presentation] In vitro増幅系を用いた発生期における肝幹/前駆細胞の機能解析2010

    • Author(s)
      紙谷聡英、岡田健、伊藤慶一、中内啓光
    • Organizer
      第17回肝細胞研究会
    • Place of Presentation
      秋田
    • Year and Date
      2010-06-18
    • Related Report
      2010 Final Research Report
  • [Presentation] In vitro増幅系を用いた発生期における肝幹/前駆細胞の機能解析2010

    • Author(s)
      紙谷聡英、岡田健、伊藤慶一、中内啓光
    • Organizer
      第17回 肝細胞研究会
    • Place of Presentation
      秋田アトリオン
    • Year and Date
      2010-06-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 胎仔肝幹・前駆細胞の新規培養系の構築2010

    • Author(s)
      紙谷聡英、岡田健、伊藤慶一、伊東秀典、中内啓光
    • Organizer
      第9回日本再生医療学会総会
    • Place of Presentation
      広島
    • Year and Date
      2010-03-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 胎仔肝幹・前駆細胞の新規培養系の構築2010

    • Author(s)
      紙谷聡英、岡田健、伊藤慶一、伊東秀典、中内啓光
    • Organizer
      第9回日本再生医療学会総会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2010-03-18
    • Related Report
      2009 Annual Research Report
  • [Book] Methods In Molecular Biology, Epithelial Cell Culture Protocols 2nd Ed.(Edited by Randell SH), "Enrichment and clonal culture of hepatic stem/progenitor cells during mouse liver development."2011

    • Author(s)
      Kamiya A, Nakauchi H
    • Publisher
      Humana Press (In press)(刊行確定)
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report
  • [Remarks] 肝細胞研究会Webページ(研究交流)

    • URL

      http://hepato.umin.jp/kouryu/kouryu13.html

    • Related Report
      2010 Final Research Report
  • [Remarks] 肝細胞研究会Webページ(研究交流)総説

    • URL

      http://hepato.umin.jp/kouryu/kouryu13.html

    • Related Report
      2010 Annual Research Report
  • [Remarks] タイトル:発生期および成体における肝幹・前駆細胞の増殖・分化誘導機構著者:紙谷聡英

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 多能性幹細胞からの分化誘導された細胞の生産方法2010

    • Inventor(s)
      中内啓光、紙谷聡英、鈴木奈穂、伊藤慶一、山崎聡
    • Industrial Property Rights Holder
      国立大学法人東京大学
    • Filing Date
      2010-12-08
    • Related Report
      2010 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 多能性幹細胞からの分化誘導された細胞の生産方法2010

    • Inventor(s)
      中内啓光、紙谷聡英、鈴木奈穂、伊藤慶一、山崎聡
    • Industrial Property Rights Holder
      国立大学法人東京大学
    • Filing Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 幹細胞の分化誘導法2009

    • Inventor(s)
      中内啓光、紙谷聡英、鈴木奈穂、伊藤慶一
    • Industrial Property Rights Holder
      国立大学法人東京大学
    • Filing Date
      2009-12-08
    • Related Report
      2009 Annual Research Report
    • Overseas

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Published: 2009-04-01   Modified: 2016-04-21  

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