Identification of MURC, muscle-restricted coiled-coil protein, and molecular characterization of MURC-induced cardiac dysfunction and arrhythmia

• Project/Area Number: 21790740
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TAGAWA Masashi Kyoto Prefectural University of Medicine, 医学研究科, 助教 (60515295)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 心筋細胞 / 心不全 / 不整脈 / MURC

Research Abstract

We identified a muscle-restricted coiled-coil protein, MURC, and found that MURC was localized to caveolae at the plasma membrane of cardiomyocytes and was associated with caveolin-3, PTRF/cavin-1, SDPR/cavin-2, and SRBC/cavin-3. We also found that overexpression of MURC in cardiomyocytes induced distension of caveolae and transgenic mice expressing MURC in the heart exhibited deformation of caveolae in cardiomyocytes. Since transgenic mice expressing MURC in the heart shows cardiac dysfunction and arrhythmia, we are investigating the molecular mechanisms responsible for MURC-induced cardiac dysfunction and arrhythmia.

Research Products

• [Journal Article] MURC, a muscle-restricted coiled-coil protein that modulates the Rho/ROCK pathway, induces cardiac dysfunction and conduction disturbance. (2008)
  • Author(s): Ogata T, Ueyama T, Isodono K, Tagawa M, Takehara N, Kawashima T, Takahashi T, Harada K, Shioi T, Matsubara H, Oh H.
  • Journal Title: Mol Cell Biol. 28 Pages: 3424-3436
  • Peer Reviewed
• [Journal Article] MURC, a muscle-restricted coiled-coil protein, is involved in the regulation of skeletal myogenesis. (2008)
  • Author(s): Tagawa M, Ueyama T, Ogata T, Takehara N, Nakajima N, Isodono K, Asada S, Takahashi T, Matsubara H, Oh H.
  • Journal Title: Am J Physiol-Cell Physiol. 295
  • Peer Reviewed
• [Presentation] A novel muscle-restricted coiled-coil protein, MURC, induces cardiac dysfunction accompanied with an altered gene expression profile. (2008)
  • Author(s): Ogata T, Ueyama T, Isodono K, Tagawa M, Takehara N, Kawashima T, Shioi T, Takahashi T, Matsubara Hiroaki, Oh H.
  • Organizer: 第72回日本循環器学会
  • Place of Presentation: 福岡
• [Presentation] A novel but evolutionarily conserved muscle-restricted coiled-coil protein, MURC, regulates myogenic differentiation in C2C12 cells. (2008)
  • Author(s): Tagawa M, Ueyama T, Ogata T, Nakajima N, Isodono K, Asada S, Takehara N, Takahashi T, Matsubara Hiroaki, Oh H.
  • Organizer: 第72回日本循環器学会
  • Place of Presentation: 福岡