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Study of an anti-tumor activity of adiponectin in a mouse lung metastasis model

Research Project

Project/Area Number 21790759
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionAsahikawa Medical College

Principal Investigator

TOYOSHIMA Eri  Asahikawa Medical College, 医学部, 助教 (20466501)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsアディポネクチン / 肺癌 / 転移
Research Abstract

We used a tumor-bearing mouse model to evaluate an anti-tumor activity of adiponectin in lung cancer cells. Our results showed that both human and mouse adiponectin inhibited growth of A549 tumors. Next, we evaluated the expression levels of adiponectin receptor, AdipoR1 and AdipoR2 in A549 cells using RT-PCR assay. The assay revealed that A549 cells expressed both AdipoR1 and AdipoR2. Then, in vitro study, A549 cells were treated with adiponectin to study whether adiponectin suppresses cell proliferation of A549 cells. Cell proliferation assay showed that adiponectin did not suppress cell proliferation of the cells. After that, we used a mouse bone-marrow transplantation model to detect the bone-marrow derived cells (BMDCs) in xenograft tumors. Bone marrow cells from GFP-transgenic mice were transplanted to the irradiated mice, and A549 cells were implanted to the mice. GFP-labeled BMDCs decreased in adiponectin-treated tumors compared with control tumors.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (5 results)

All 2009 2008 Other

All Presentation (4 results) Remarks (1 results)

  • [Presentation] Adiponectin inhibits growth of non-small cell lung cancer cells and suppresses recruitment of bone-marrow-derived cells to the tumor in vivo2009

    • Author(s)
      Shunsuke Okumura
    • Organizer
      Internal association for the study of lung cancer
    • Place of Presentation
      San Francisco
    • Year and Date
      2009-08-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Adiponectin inhibits the growth of non-small cell lung cancer cells and suppresses recruitment of bone-marrow-derived cells to the tumors in vivo.2009

    • Author(s)
      Okumura,Toyoshima, et al.
    • Organizer
      IASLC
    • Place of Presentation
      San Francisco
    • Related Report
      2010 Final Research Report
  • [Presentation] アディポカインの非小細胞肺癌に対する腫瘍増殖抑制効果の検討2008

    • Author(s)
      奥村、豊嶋, ら
    • Organizer
      北海道腫瘍系分科会
    • Place of Presentation
      旭川
    • Related Report
      2010 Final Research Report
  • [Presentation] アディポカインの非小細胞肺癌細胞株に対する抗腫瘍効果の検討2008

    • Author(s)
      奥村、豊嶋, ら
    • Organizer
      肺癌学術講演会
    • Place of Presentation
      北九州
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等 なし

    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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