The effect of 5-aminoimidazole-4-carboxamide ribonucleoside (AMPK)on malignant mesothelioma cell growth and survival interval in an implant animal model of malignant mesothelioma

• Project/Area Number: 21790763
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Tohoku University
• Principal Investigator: NIU Kaijun 東北大学, 大学院・医工学研究科, 准教授 (70447134)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 非閉塞性肺疾患癌 / 肺線維症 / 呼吸器感染症 / ヒト悪性中皮腫 / 悪性中皮腫 / AMPK活性 / AICAR / SCIDマウス / 細胞周期 / アポトーシス / AMPK活性化

Research Abstract

In vitro, AICAR treatment significantly impaired the cell proliferation rate of malignant mesothelioma (MM)cell lines. In vivo, treatment with AICAR significantly decreased the growth rate of MM, which was accompanied by an increase in p53/phosphor-p53 levels, and down-regulated protein levels of phosphor-Akt and p70/phosphor-p70. Similar intracellular pathways are also observed in vitro experiment. These effects partly involved induction of growth arrest and apoptosis to MM cells. AICAR treatment activated AMPK. Finally, AICAR treatment also significantly extended survival interval in an implant animal model of MM. These findings suggested that AICAR or the induction of AMPK activity is an important tactic for developing therapeutic strategies against MM.

Research Products

• [Journal Article] Adiponectin pathway attenuates malignant mesothelioma cell growth. (2012)
  • Author(s): Niu K, Asada M, et al
  • Journal Title: Am J Respir Cell Mol Biol. Volume: 46 Pages: 515-23
  • DOI: 10.1165/rcmb.2011-0068oc
  • Peer Reviewed
• [Journal Article] Adiponectin pathway attenuates malignant mesothelioma cell growth (2011)
  • Author(s): Kaijun Niu, Masanori Asada, Tatsuma Okazaki, Shinsuke Yamanda, Takae Ebihara, Hui Guo, Dongying Zhang, Ryoichi Nagatomi, Hiroyuki Arai, Masahiro Kohzuki, and Satoru Ebihara
  • Journal Title: Am J Respir Cell Mol Biol
  • Peer Reviewed
• [Presentation] AMP活性化プロテインキナーゼ活性化と悪性中皮腫 (2010)
  • Author(s): 牛凱軍、海老原覚、浅田成紀、矢満田慎介、岡崎達馬、荒井啓行、永富良一
  • Organizer: 第50回日本呼吸器学会学術講演会
  • Place of Presentation: 京都・国立京都国際会館
  • Year and Date: 2010-04-24
• [Remarks]
  • URL: http://db.tohoku.ac.jp/whois/detail/335591bd83c5e95f47e756e87c06e8c9.html
• [Remarks]
  • URL: http://db.tohoku.ac.jp/Whois/detail/335591bd83c5e95f47e756e87c06e8c9.html
• [Remarks]
  • URL: http://db.tohoku.ac.jp/whois/detail/335591bd83c5e95f47e756e87c06e8c9.html