|Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
To determine whether SHP-1 is a therapeutic target molecule for renal anemia, SHP-1 siRNA induction was performed to knockdown SHP-1 expression in renal anemia model mice. The c-kit positive hematopoietic progenitor cells were isolated from bone marrow of 5/6 nephrectomized mice, and transfection of the c-kit positive hematopoietic progenitor cells with SHP-1 siRNA and shRNA was attempted. Eventually, the experiment was not successful maybe due to the condition for transfection or cell viability.