Research Project
Grant-in-Aid for Young Scientists (B)
To clarify the molecular mechanisms of GCI formation and subsequent oligodendroglial degeneration, we ectopically expressed α-SYN and TPPP in HEK293T and oligodendroglial KG1C cell lines. Here we showed that TPPP specifically accelerated α-SYN oligomer formation and co-immunoprecipitation analysis revealed the specific interaction of TPPP and α-SYN. Moreover, phosphorylation of α-SYN at Ser-129 facilitated the TPPP-mediated α-SYN oligomerization. TPPP facilitated α-SYN-positive cytoplasmic perinuclear inclusions mimicking GCI in both cell lines ; however, apoptotic cell death was only observed in KG1C cells. This apoptotic cell death was partly rescued by sirtuin 2 inhibition.
All 2010 2009 Other
All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (6 results) Remarks (1 results)
Neurochem Int 57
Pages: 857-66
Brain 133
Pages: 1772-1778
Neurochemisty International
Volume: 57 Pages: 857-866
Int J Mol Sci
Volume: 11 Pages: 1082-1089
Brain
Volume: 133 Pages: 1772-1778
Int J Mol Sci 11
Pages: 1082-9
J Neurol Sci 15
Pages: 36-9
Hum Mol Genet 18
Pages: 3832-50
Neuroscience 160
Pages: 796-804
http://www.neurol.med.tohoku.ac.jp/