Molecular genetic analyses of the CYP11B2 gene in cardiovascular tissues
Project/Area Number |
21790889
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Endocrinology
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Research Institution | Kanazawa University |
Principal Investigator |
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | アルドステロン / エピジェネティクス / DNAメチル化 / 組織特異的プロモーター / CYP11B2 / 核内受容体 / 心筋症 / 心肥大 / 肥大型心筋症 |
Research Abstract |
In addition to the primary effect of aldosterone to induce sodium and fluid retention, excess aldosterone has direct inflammatory and fibrotic effects contributing to target organ deterioration, leading to the development of vascular, renal, and cardiac inflammation ; fibrosis ; and hypertrophy. Although the adrenal gland is a major source of aldosterone, aldosterone production and levels of mRNA coding for CYP11B2 are induced in the ventricles during hypertrophy, and aldosterone synthesis from heart itself causes cardiac hypertrophy. We showed a role for DNA methylation in CYP11B2 gene repression and suggest an epigenomic mechanism may be causally linked with an increase in cardiac aldosterone production in the pathophysiology of the heart. Meanwhile, nuclear receptors(NGFIB, NURR1, NOR1, SF1) involved in aldosteronogenesis differentially employed multiple alternative promoters(APs) in a tissue-specific manner. The use of APs was different between human adrenal and cardiovascular tissues.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] A Humanized Pattern of Aromatase Expression Is Associated with Mammary Hyperplasia in Mice2012
Author(s)
Zhao H, Pearson EK, Brooks DC, Coon V JS, Chen D, Demura M, Zhang M, Clevenger CV, Xu X, Veenstra TD, Chatterton RT, Demayo FJ, Bulun SE.
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Journal Title
Endocrinology
Volume: (in press)
Issue: 6
Pages: 2701-2713
DOI
Year and Date
2012-04-16
Related Report
Peer Reviewed
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[Journal Article] Multiple noncoding exons 1 of nuclear receptors NR4A family(nerve growth factor-induced clone B, Nur-related factor 1 and neuron-derivedorphan receptor 1) and NR5A1(steroidogenic factor 1) in human cardiovascular and adrenal tissues2011
Author(s)
Demura M, Wang F, Yoneda T, Karashima S, Mori S, Oe M, Kometani M, Sawamura T, Cheng Y, Maeda Y, Namiki M, Ino H, FujinoN, Uchiyama K, Tsubokawa T, Yamagishi M, Nakamura Y, Ono K, Sasano H, Demura Y, Takeda Y
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Journal Title
J Hypertens
Volume: 29
Issue: 6
Pages: 1185-95
DOI
Related Report
Peer Reviewed
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[Presentation] Epigenomic alterations activate the CYP11B2(aldosterone synthase) gene in cardiomyopathy2011
Author(s)
Fen Wang, Masashi Demura, Takashi Yoneda, Shunsuke Mori, Masashi Oe, Yuan Cheng, Hidekazu Ino, Noboru Fujino, Uchiyama Katsuharu, Toshinari Tsubokawa, Masakazu Yamagishi, Yasuhiro Nakamura, Katsuhiko Ono
Organizer
The 4^<th> International Aldosterone Forum in Japan
Place of Presentation
Grand Hall, Tokyo
Year and Date
2011-05-14
Related Report
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[Presentation] Multiple noncoding exons 1 of nuclear receptors NR4A family(NGFIB, NURR1, NOR1) and NR5A1(SF1) in human vascular and adrenal tissues2011
Author(s)
Masashi Demura, Fen Wang, Takas Yoneda, Shigehiro Karashima, Shunsuke Mori, Mistuhiro Kometani, Masashi Oe, Yuan Cheng, Yuji Maeda, Mikio Namiki, Katsuhiko Ono, Yasuhiro Nakamura, Hironobu Sasano, Kiyofumi Saijoh, Yoshiyu Takeda
Organizer
The Endocrine Society's 93^<th> Annual Meeting, the Boston Convention & Exhibition Center
Place of Presentation
Boston, MA, USA
Related Report
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[Presentation] Epigenetic regulation of the2010
Author(s)
Fen Wang, Masashi Demura, Takashi Yoneda, Shigehiro Karashima, Shunsuke Mori, Mistuhiro Kometani, Masashi Oe, Yuan Cheng, KatsuharuUchiyama, Chiaki Nakanishi, Toshinari Tsubokawa, Hidekazu Ino, Noboru Fujino, Masakazu Yamagishi, Yuji Maeda, Mikio Namiki, Yoshiyu Takeda
Organizer
aldosterone synthase CYP11B2 gene 23^<rd> Scientific Meeting of the International Society of Hypertension
Place of Presentation
Vancouver Convention Centre, Canada
Related Report
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[Presentation] Effect of DNA Methylation on Aldosterone Synthase Gene2010
Author(s)
Masashi Demura, Fen Wang, Takashi Yoneda, Shigehiro Karashima, Shunsuke Mori, Mistuhiro Kometani, Masashi Oe, Yuan Cheng, Katsuharu Uchiyama, Masakazu Yamagishi, Yuji Maeda, Mikio Namiki, Yoshiyu Takeda
Organizer
The Endocrine Society's 92^<th> Annual Meeting, San Diego Convention Center
Place of Presentation
San Diego, CA, USA
Related Report
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[Presentation] Multiple noncoding first exons of nuclear receptors NR4A family(NGFIB, NURR1, NOR1) and NR5A1(SF1) in human adrenal and vascular tissues2010
Author(s)
Masashi Demura, Fen Wang, Takashi Yoneda, Shigehiro Karashima, Shunsuke Mori, Mistuhiro Kometani, Masashi Oe, Yuan Cheng, Yuji Maeda, Mikio Namiki, Masakazu Yamagishi, Yoshiyu Takeda
Organizer
The 3^<rd> International Aldosterone Forum in Japan
Place of Presentation
Grand Hall, Tokyo
Related Report
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[Presentation] Epigenetics in aldosteroneproduction2010
Author(s)
Masashi Demura, Fen Wang, Takashi Yoneda, Shigehiro Karashima, Shunsuke Mori, Mistuhiro Kometani, Masashi Oe, Yuan Cheng, Katsuharu Uchiyama, Chiaki Nakanishi, Toshinari Tsubokawa, Hidekazu Ino, Noboru Fujino, Masakazu Yamagishi, Yuji Maeda, Mikio Namiki,Yoshiyu Takeda
Organizer
International Symposium on Cardiovascular Endocrinology and Metabolism CVEM 2010
Place of Presentation
Nara Prefectural New Public Hall, Nara
Related Report
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[Presentation] Epigenetic regulation of aldosterone production2010
Author(s)
Masashi Demura, Fen Wang, Takashi Yoneda, Shigehiro Karashima, Yuan Cheng, Yoshiyu Takeda
Organizer
International Symposium for Aldosterone and Related Substances in Hypertension(ISARSH 2010)
Place of Presentation
Hotel Metropolitan Sendai, Sendai, Japan
Related Report
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