Comprehensive survey of microRNAs and mRNAs involved in cutaneous squamous cell carcinoma invasion and metastasis

• Project/Area Number: 21791085
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: University of the Ryukyus
• Principal Investigator: TAKEI Kimiko University of the Ryukyus, 大学院・医学研究科, 助教 (90325861)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 癌 / ゲノム / シグナル伝達 / シグナル伝逹

Research Abstract

In order to understand the mechanism of invasion and metastasis of cutaneous squamous cell carcinoma (cSCC), we have compared microRNA, mRNA and protein expression profiles between metastatic and non-metastatic transformed keratinocyte strains. Metastatic cells exhibited ectopic overexpression of the keratin 8/18 pair, and non-metastatic cells gained invasiveness after expression of exogenous keratin 8/18. Consistentlym an immunohistological analyses of these keratins in cSCC speciments revealed a significant correlation between ecctopic keratin 8/18 and invasiveness.

Research Products

• [Journal Article] Ectopic coexpression of keratin 8 and 18 promotes invasion of transformed keratinocytes and is induced in patients with cutaneous squamous cell carcinoma. (2010)
  • Author(s): Yamashiro, Y., Takei, K., Umikawa, M., Asato, T., Oshiro, M., Uechi, Y., Ishikawa, T., Taira, K., Uezato, H., Kariya, K.
  • Journal Title: Biochem.Biophys.Res.Commun. 399 Pages: 365-372
  • Peer Reviewed
• [Journal Article] Phaeohyphomycosis caused by Exophiala jeanselmei in a patient with polymyalgia rheumatica. (2010)
  • Author(s): Arakaki, O., Asato, Y., Yagi, N., Taira, K., Yamamoto, Y., Takei, K., Hosokawa, A., Kayo, S., Hagiwara, K., Uezato, H.
  • Journal Title: J.Dermatol. 37 Pages: 363-373
• [Journal Article] Ectopic coexpression of keratin 8 and 18 promotes invasion of transformed keratinocytes and is induced in patients with cutaneous squamous cell carcinoma. (2010)
  • Author(s): Yamashiro, Y., et al.
  • Journal Title: Biochem Biophys Res Commun Volume: 399 Pages: 365-372
  • Peer Reviewed
• [Presentation] The proteomic search for prognosis markers of cutaneous squamous cell carcinoma. (2009)
  • Author(s): Yoshito Yamashiro(代表発表者), Kimiko Takei, Masato Umikawa, Tsuyoshi Asato, Kiyohito Taira, Hiroshi Uezato, Ken-ichi Kariya
  • Organizer: MBSJ2009(第32回日本分子生物学会年会)プログラム:3P-0743:418
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] プロテオミクス解析による有棘細胞癌予後マーカーの探索 (2009)
  • Author(s): 山城義人, 他
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] Localization of Rap2 in recycling endosome in a palmitoylation-dependent manner. (2008)
  • Author(s): Maitsetseg Bayarjargal(代表発表者), Yukiko Uechi, Shogo Endo, Kimiko Nonaka-Takei, Minoru Oshiro, Masato Umikawa, Tsuyoshi Asato, Yoshito Yamashiro, Tomohiko Taguchi, Ken-ichi Kariya
  • Organizer: Biochemistry Molecular Biology(BMB)2008(第31回日 本分子生物学会年会・第81回日本生化学会大会合同大会)プログラム:1P-0444:219
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-09