A role of mast cell-derived IL-31 in pruritus in atopic dermatitis

• Project/Area Number: 21791099
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Juntendo University
• Principal Investigator: HARA Mutsuko Juntendo University, 医学(系)研究科, 研究員 (60420832)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: IL-31 / 皮膚炎 / マスト細胞 / Il-31 / かゆみ / アトピー性皮膚炎 / I1-31

Research Abstract

In this study, we tested our hypothesis that mast cells might produce an pruritic sytokine IL-31 and play a role in prutitus in atopic dermatitis. We found that anti-microbial peptides induced IL-31 production from human mast cells and IL-31 expression was detected in mast cells in skin lesions of a mouse model of atopic dermatitis. Collectively, these results suggest that mast cell-derived IL-31 may play a role in pruritus in atopic dermatitis.

Research Products

• [Journal Article] Circadian clock gene Period2 regulates a time-of-day-dependent variation in cutaneous anaphylactic reaction. (2011)
  • Author(s): Nakamura Y, Harama D, Shimokawa N, Hara M, Suzuki R, Tahara Y, Ishimaru K, Katoh R, Okumura K, Ogawa H, Shibata S, Nakao A.
  • Journal Title: J Allergy Clin Immunol. 127(4)
  • Peer Reviewed
• [Journal Article] Critical role of transcription factor PU.1 in the expression of CD80 and CD86 on dendritic cells. (2011)
  • Author(s): Kanada S, Nishiyama C, Nakano N, Suzuki R, Maeda K, Hara M, Kitamura N, Ogawa H, Okumura K.
  • Journal Title: blood 117(7) Pages: 2211-2222
  • Peer Reviewed
• [Journal Article] The circadian clock gene Period2 regulates a time of day-dependent variation in cutanenous anaphylactic reaction. (2011)
  • Author(s): Nakamura Y, et al.
  • Journal Title: J Allergy Clin Immun Volume: (In press)
  • Peer Reviewed
• [Journal Article] Critical role of transcription factor PU.1 in the expression of CD80 and CD86 on dendritic cells. (2011)
  • Author(s): Kanada S, et al.
  • Journal Title: Blood Volume: 117 Pages: 2211-2222
  • Peer Reviewed
• [Journal Article] Establishment of a simple detection system for blood group ABO-specific transferase activity in DNA-transfected cells. (2010)
  • Author(s): Nishida C, Nishiyama C, Satoh K, Hara M, Itoh Y, Ogawa H, Okumura K.
  • Journal Title: Leg Med (Tokyo) 12(4) Pages: 172-176
  • NAID: 10027575992
  • Peer Reviewed
• [Journal Article] Suppressive effects of transcription factor GATA-1 on cell type-specific gene expression in dendritic cells. (2010)
  • Author(s): Shimokawa N, Nishiyama C, Nakano N, Maeda K, Suzuki R, Hara M, Fukai T, Tokura T, Miyajima H, Nakao A, Ogawa H, Okumura K.
  • Journal Title: 62(7) Pages: 421-429
  • Peer Reviewed
• [Journal Article] Opposite effects of Trichostatin A on activation of mast cells by different stimulants. (2010)
  • Author(s): Wang QH, Nishiyama C, Nakano N, Kanada S, Hara M, Kitamura N, Shimokawa N, Lu CL, Ogawa H, Okumura K.
  • Journal Title: FEBS Let. 584(11) Pages: 2315-2320
  • Peer Reviewed
• [Journal Article] Antimicrobial peptides human beta-defensins and cathelicidin LL-37 induce the secretion of a pruritogenic cytokine IL-31 by human mast cells. (2010)
  • Author(s): Niyonsaba F, Ushio H, Hara M, Yokoi H, Tominaga M, Takamori K, Kajiwara N, Saito H, Nagaoka I, Ogawa H, Okumura K
  • Journal Title: J Immunol 184(7) Pages: 3526-3534
  • Peer Reviewed
• [Journal Article] Establishment of a simple detection system for blood group ABO-specific transferase activity in DNA-transfected cells. (2010)
  • Author(s): Nishida C, et al.
  • Journal Title: Leg Med Volume: 12 Pages: 172-176
  • Peer Reviewed
• [Journal Article] Opposite effects of Trichostatin A on activation of mast cells by different stimulants. (2010)
  • Author(s): Wang QH, et al.
  • Journal Title: FEBS Lett Volume: 584 Pages: 2315-2320
  • Peer Reviewed
• [Journal Article] Suppressive effects of transcription factor GATA-1 on cell type-specific gene expression in dendritic cells. (2010)
  • Author(s): Shimokawa N, et al.
  • Journal Title: Immunogenetics (In press)
  • Peer Reviewed
• [Journal Article] FcepsilonRIalpha gene-18483A>C polymorphism affects transcriptional activity through YY1 binding. (2009)
  • Author(s): Potaczek DP, Maeda K, Wang QH, Nakano N, Kanada S, Stepien E, Branicka A, Fukai T, Hara M, Tokura T, Ogawa H, Undas A, Okumura K, Nishiyama C.
  • Journal Title: Immunogenetics. 61(9) Pages: 649-655
  • Peer Reviewed
• [Journal Article] Involvement of PU.1 in the transcriptional regulation of TNF-alpha. (2009)
  • Author(s): Fukai T, Nishiyama C, Kanada S, Nakano N, Hara M, Tokura T, Ikeda S, Ogawa H, Okumura K
  • Journal Title: Biochem Biophys Res Commun. 388(1) Pages: 102-106
  • Peer Reviewed
• [Journal Article] Roles of PU.1 in monocyte- and mast cell-specific gene regulation : PU.1 transactivates CIITA pIV in cooperation with IFN-gamma. (2009)
  • Author(s): Ito T, Nishiyama C, Nakano N, Nishiyama M, Usui Y, Takeda K, Kanada S, Fukuyama K, Akiba H, Tokura T, Hara M, Tsuboi R, Ogawa H, Okumura K.
  • Journal Title: Int Immunol. 21(7) Pages: 803-816
  • Peer Reviewed
• [Journal Article] Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. (2009)
  • Author(s): Kinoshita H, Takai T, Le TA, Kamijo S, Wang XL, Ushio H, Hara M, Kawasaki J, Vu AT, Ogawa T, Gunawan H, Ikeda S, Okumura K, Ogawa H.
  • Journal Title: J Allergy Clin Immunol. 123(1) Pages: 179-186
  • Peer Reviewed
• [Journal Article] Antimicrobial Peptides Human {beta}-Defensins and Cathelicidin LL-37 Induce the Secretion of a Pruritogenic Cytokine IL-31 by Human Mast Cells. (2009)
  • Author(s): Niyonsaba F, et al.
  • Journal Title: J Immunol. (In press)
  • Peer Reviewed
• [Journal Article] FcepsilonRIalpha gene -18483A>C polymorphism affects transcriptional activity through YY1 binding (2009)
  • Author(s): Potaczek DP, et al.
  • Journal Title: Immunogenetics. 61 Pages: 649-55
  • Peer Reviewed
• [Journal Article] Involvement of PU.1 in the transcriptional regulation of TNF-alpha. (2009)
  • Author(s): Fukai T, et al.
  • Journal Title: Biochem Biophys Res Commun. 388 Pages: 102-106
  • Peer Reviewed
• [Journal Article] Roles of PU.1 in monocyte- and mast cell-specific gene regulation : PU.1 transactivates CIITA pIV in cooperation with IFN-gamma. (2009)
  • Author(s): Ito T, et al.
  • Journal Title: Int Immunol 21 Pages: 803-816
  • Peer Reviewed
• [Journal Article] Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. (2009)
  • Author(s): Kinoshita H., et al.
  • Journal Title: J Allergy Clin Immunol. 123 Pages: 179-186
  • Peer Reviewed
• [Presentation] 転写調節因子PU.1による樹状細胞遺伝子発現制御機構解析と自己免疫疾患治療応用 (2011)
  • Author(s): 西山千春, 他
  • Organizer: 日本農芸化学会2011年度大会
• [Presentation] 小児アレルギー疾患における末梢血好塩基球および樹状細胞の高親和性IgE受容体(FcεRI)発現の検討 (2010)
  • Author(s): 鈴木竜洋, 他
  • Organizer: 日本小児アレルギー学会
  • Place of Presentation: 横浜(パシフィコ横浜 会議センター)
  • Year and Date: 2010-12-04
• [Presentation] アレルギー性気道炎症モデルにおけるアレルゲンへの自然免疫細胞応答の関与 (2010)
  • Author(s): 上條清嗣, 他
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京(東京国際フォーラム)
  • Year and Date: 2010-11-25
• [Presentation] 小児アレルギー疾患における末梢血好塩基球および樹状細胞の高親和性IgE受容体(FceRI)発現の検討 (2010)
  • Author(s): 鈴木竜洋, 他
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京(東京国際フォーラム)
  • Year and Date: 2010-11-25
• [Presentation] involvement of innate immune cell response to allergens in the pathogenesis of allergic airway inflammation (2010)
  • Author(s): S.Kamijo, 他
  • Organizer: 第14回国際免疫学会
  • Place of Presentation: 神戸(神戸ポートピアホテル)
  • Year and Date: 2010-08-27
• [Remarks] ホームページ等
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/atopy_center/index.html
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/saisentan/index.html
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/kyodo_kenkyu_kensyu/index.html