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Involvement of the microenvironment on the radiosensitivity

Research Project

Project/Area Number 21791190
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Radiation science
Research InstitutionKyoto University

Principal Investigator

KASHINO Genro  Kyoto University, 原子炉実験所, 助教 (00437287)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords微小環境 / バイスタンダー効果 / ミトコンドリア / 活性酸素 / 突然変異 / 53BP1 / 放射線感受性 / 分泌因子 / DNA二重鎖切断
Research Abstract

It has been suggested that tumor microenvironment is important for the therapy. In this study, we examined the effects of secreted factors from irradiated cells on the treated cells to check the microenvironment after irradiation. The results suggest that membrane activity of mitochondria was reduced, and the level of reactive oxygen species was increased by the treatments of the secreted factors. Also, mutation fraction was increased in cells treated with secreted factors. However, these effects were not affected in radiosensitivity indicated by the foci formation of 53BP1 after X-irradiation.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (32 results)

All 2010 2009

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (14 results)

  • [Journal Article] Usefulness of hexamethylenetetramine as an adjuvant to radiation and cisplatin in the treatment of solid tumors : its independency of p53 status2010

    • Author(s)
      Masunaga S, Tano K, Nakamura J, Watanabe M, Kashino G, Takahashi A, Tanaka H, Suzuki M, Ohnishi K, Kinashi Y, Liu Y, Ohnishi T, Ono K.
    • Journal Title

      J.Radiat.Res. 51

      Pages: 27-35

    • NAID

      10025914038

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of manipulating hypoxia in solid tumors on the radiation dose-rate effect in vivo, with reference to that in the quiescent cell population.2010

    • Author(s)
      Masunaga S, Hirayama R, Uzawa A, Kashino G, Takata T, Tanaka H, Suzuki M, Kinashi Y, Liu Y, Koike S, Ando K, Ono K.
    • Journal Title

      Jap.J.Raiol. 28

      Pages: 132-142

    • NAID

      120002511344

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] An alternative mechanism for radioprotection by dimethyl sulfoxide; possible facilitation of DNA double-strand break repair.2010

    • Author(s)
      Kashino G, Liu Y, Suzuki M, Masunaga S, Kinashi Y, Ono K, Tano K, Watanabe M
    • Journal Title

      J.Radiat.Res. 51

      Pages: 733-740

    • NAID

      10026964239

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ascorbic acid 2-glucocide reduces micronucleus induction in distant splenic T lymphocytes following head irradiation.2010

    • Author(s)
      Kinashi Y, Tanaka H, Masunaga S, Suzuki M, Kashino G, Yong L, Takahashi S, Ono K.
    • Journal Title

      Mutat.Res. 695

      Pages: 69-74

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] An alternative mechanism for radioprotection by dimethyl sulfoxide ; possible facilitation of DNA double-strand break repair.2010

    • Author(s)
      Kashino G, Liu Y, Suzuki M, Masunaga S, Kinashi Y, Ono K, Tano K, Watanabe M
    • Journal Title

      Journal of Radiation Research

      Volume: 51 Pages: 733-740

    • NAID

      10026964239

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inductions of DNA double strand breaks and cellular migrations through the bystander effects in the cells irradiated with slit type microplanar beam of the SPring-8 synchrotron.2009

    • Author(s)
      Kashino G, Kondoh T, Nariyama N, Umetani K, Ohigashi T, Shinohara K, Kurihara A, Fukumoto M, Tanaka H, Maruhashi A, Suzuki M, Kinashi Y, Liu Y, Masunaga S, Watanabe M, Ono K
    • Journal Title

      Int.J Radiat.Oncol.Biol.Physic. 74

      Pages: 229-236

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spectromicroscopic film dosimetry for high-energy microbeam from synchrotron radiation.2009

    • Author(s)
      Nariyama N, Ohigashi T, Umetani K, Shinohara K, Tanaka H, Maruhashi A, Kashino G, Kurihara A, Kondob T, Fukumoto M, Ono K
    • Journal Title

      Appl.Radiat.Isot. 67

      Pages: 155-159

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Watanabe M, Phosphorylated H2AX foci in tumor cells have no correlation with their radiation sensitivities.2009

    • Author(s)
      Yoshikawa T, Kashino G, Ono K
    • Journal Title

      J.Radiat.Res. 50

      Pages: 151-160

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Usefulness of hexamethylenetetramine in combination with chemotherapy using free and pegylated liposomal doxorubicin in vivo, referring to the effect on quiescent cells2009

    • Author(s)
      Masunaga S, Kono K, Nakamura J, Tano K, Yoshida H, Watanabe M, Kashino G, Suzuki M, Kinashi Y, Liu Y, Ono K.
    • Journal Title

      Oncol.Rep. 21

      Pages: 1307-1312

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nakamura J : Evaluation of the potential of hexamethylenetetramine, compared with tirapazamine, as a combined agent with {gamma}-irradiation and cisplatin treatment in vivo.2009

    • Author(s)
      Masunaga S, Tano K, Watanabe M, Kashino G, Suzuki M, Kinashi Y, Ono K
    • Journal Title

      Br.J.Radiol. 82

      Pages: 392-400

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effect of post-irradiation tumor oxygenation status on recovery from radiation-induced damage in vivo : with reference to that in quiescent cell populations.2009

    • Author(s)
      Masunaga SI, Hirayama R, Uzawa A, Kashino G, Suzuki M, Kinashi Y, Liu Y, Koike S, Ando K, Ono K
    • Journal Title

      J.Cancer Res.Clin.Oncol. 135

      Pages: 1109-11016

    • NAID

      120001624124

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impact of accelerator-based boron neutron capture therapy(AB-BNCT)on the treatment of multiple liver tumors and malignant pleural mesothelioma.2009

    • Author(s)
      Suzuki M, Tanaka H, Sakurai Y, Kashino G, Yong L, Masunaga S, Kinashi Y, Mitsumoto T, Yajima S, Tsutsui H, Sato T, Maruhashi A, Ono K
    • Journal Title

      Radiother.Oncol. 92

      Pages: 89-95

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Simple and Rapid Method for Measurement of 10B-para-Boronophenylalanine in the Blood for Boron Neutron Capture Therapy Using Fluorescence Spectrophotometry.2009

    • Author(s)
      Kashino G, Fukutani S, Suzuki M, Liu Y, Nagata K, Masunaga SI, Maruhashi A, Tanaka H, Sakurai Y, Kinashi Y, Fujii N, Ono K
    • Journal Title

      J.Radiat.Res. 50

      Pages: 377-382

    • NAID

      10025912421

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gamma-ray irradiation enhanced boron-10 compound accumulation in murine tumors.2009

    • Author(s)
      Liu Y, Nagata K, Masunaga S, Suzuki M, Kashino G, Kinashi Y, Tanaka H, Sakurai Y, Maruhashi A, Ono K.
    • Journal Title

      J.Radiat.Res. 50

      Pages: 553-557

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-term persistence of X-ray-induced genomic instability in quiescent normal human diploid cells.2009

    • Author(s)
      Suzuki K, Kashino G, Kodama S, Watanabe M
    • Journal Title

      Mutat.Res. 671

      Pages: 33-39

    • NAID

      120006983991

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inductions of DNA double strand breaks an cellular migrations through the bystander effects in the cells irradiated with slit type microplanar beam of the SPring-8 synchrotron.2009

    • Author(s)
      Kashino G, Kondoh T, Nariyama N, Umetani K, Ohigashi T, Shinohara K, Kurihara A, Fukumoto M, Tanaka H, Maruhashi A, Suzuki M, Kinashi Y, Liu Y, Masunaga S, Watanabe M, Ono K.
    • Journal Title

      International Journal of Radiation Oncology, Biology, Physics 74

      Pages: 229-236

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylated H2AX foci in tumor cells have no correlation with their radiation sensitivities.2009

    • Author(s)
      Yoshikawa T, Kashino G, Ono K, Watanabe M.
    • Journal Title

      Journal of Radiation Research 50

      Pages: 151-160

    • NAID

      10025911507

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term persistence of X-ray-induced genomic instability in quiescent normal human diploid cells.2009

    • Author(s)
      Suzuki K, Kashino G, Kodama S, Watanabe M.
    • Journal Title

      Mutation Research 671

      Pages: 33-39

    • NAID

      120006983991

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 放射線誘発バイスタンダー効果による突然変異誘発機構2010

    • Author(s)
      菓子野元郎、熊谷純、田野恵三、渡邉正己
    • Organizer
      日本放射線影響学会第53回大会
    • Place of Presentation
      京都
    • Year and Date
      2010-10-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 放射線誘発バイスタンダー効果による突然変異誘発機構2010

    • Author(s)
      菓子野元郎、熊谷純、田野恵三、渡邊正己
    • Organizer
      日本放射線影響学会第53回大会
    • Place of Presentation
      京都市
    • Year and Date
      2010-10-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 放射線照射による分泌性因子を介した突然変異誘発の機構解明2010

    • Author(s)
      菓子野元郎、熊谷純、田野恵三、渡邉正己
    • Organizer
      日本組織培養学会第81回大会
    • Place of Presentation
      岡山
    • Year and Date
      2010-05-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 放射線照射による分泌性因子を介した突然変異誘発の機構解明2010

    • Author(s)
      菓子野元郎、熊谷純、田野恵三、渡邉正己
    • Organizer
      日本組織培養学会第81回大会
    • Place of Presentation
      岡山市
    • Year and Date
      2010-05-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 放射線により誘導される分泌性因子を介した突然変異生成機構2010

    • Author(s)
      菓子野元郎、熊谷純、渡邉正己、小野公二
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Slow releasing long lived radicals are involved in the bystander mutagenesis response.2010

    • Author(s)
      Genro Kashino, Jun Kumagai, Hiroyuki Kugoh, Mitsuo Oshimura, Keizo Tano, Koji Ono, Masami Watanabe
    • Organizer
      56th Annual Meeting of the Radiation Research Society
    • Place of Presentation
      米国ハワイ州マウイ
    • Related Report
      2010 Final Research Report
  • [Presentation] 分泌性因子を介したバイスタンダー効果による突然変異誘発機構2009

    • Author(s)
      菓子野元郎、熊谷純、劉勇、鈴木実、木梨友子、増永慎一郎、渡邉正己、小野公二
    • Organizer
      日本放射線影響学会第52回大会
    • Place of Presentation
      広島市
    • Year and Date
      2009-11-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 分泌因子を介したバイスタンダー効果による突然変異誘発機構2009

    • Author(s)
      菓子野元郎、熊谷純、劉勇、鈴木実、木梨友子、増永慎一郎、渡邉正己、小野公二
    • Organizer
      日本放射線影響学会第52回大会
    • Place of Presentation
      広島
    • Year and Date
      2009-11-11
    • Related Report
      2010 Final Research Report
  • [Presentation] SPring-8放射光のスリット状照射による細胞への影響2009

    • Author(s)
      菓子野元郎、鈴木雅雄、近藤威、梅谷啓二、成山展照、福本学、劉勇、鈴木実、増永慎一郎、木梨友子、田中浩基、丸橋晃、渡邉正己、小野公二
    • Organizer
      第48回日本医学放射線学会生物部会学術大会
    • Place of Presentation
      富山市
    • Year and Date
      2009-07-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] 放射線誘発バイスタンダー効果におけるラジカル種の関与2009

    • Author(s)
      菓子野元郎、熊谷純、劉勇、木梨友子、鈴木実、増永慎一郎、小野公二、渡邉正己
    • Organizer
      日本保健物理学会第43回研究発表会
    • Place of Presentation
      大阪市
    • Year and Date
      2009-06-03
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Involvement of radical species on radiation induced bystander effects.2009

    • Author(s)
      Genro Kashino
    • Organizer
      The 2nd Asian Congress of Radiation Research
    • Place of Presentation
      ソウル市
    • Year and Date
      2009-05-19
    • Related Report
      2010 Final Research Report
  • [Presentation] Involvement of radical species on radiation induced bystander effects2009

    • Author(s)
      Genro Kashino
    • Organizer
      The 2^<nd> Asian Congress of Radiation Research
    • Place of Presentation
      ソウル市(KOREA)
    • Year and Date
      2009-05-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] The role of radicals in bystander effect through the secreted factors from irradiated cells.2009

    • Author(s)
      Genro Kashino, Jun Kumagai, Koji Ono, Masami Watanabe
    • Organizer
      55th Annual Meeting of the Radiation Research Society
    • Place of Presentation
      米国ジョージア州サバンナ
    • Related Report
      2010 Final Research Report
  • [Presentation] The role of radicals in bystander effect through the secreted factors from irradiated cells2009

    • Author(s)
      Genro Kashino, Jun Kumagai, Koji Ono, Masami Watanabe
    • Organizer
      55th Annual Meeting of the Radiation Research Society
    • Place of Presentation
      米国ジョージア州サバンナ
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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