Identification of synovial sarcoma stem cell as a new therapeutic target
| Project/Area Number |
21791383
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIMURA Taichi Hokkaido University, 大学院・医学研究科, 助教 (90435959)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 滑膜肉腫 / 癌幹細胞 / 腫瘍幹細胞 / Sphere cultur / Side population / Sphere culture |
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| Research Abstract |
We attempted to identify a synovial sarcoma stem cell with sphere culture. The higher expression of stemness genes, Oct3, Nanog, and Sox2 was observed in the sphere subpopulation, and higher tumorigenicity was also observed in the xeno-plantation made from sphere subpopulation. Next, we compare the gene expression profiles between sphere and non-sphere subpopulation and determined candidate genes of the stem cell marker. Using the specific antibodies and cell sorter, we divided candidate gene positive and negative subpopulation, and analyzed the difference of tumorigenicity. We conformed that candidate gene positive subpopulation possess higher tumorigenicity than negative one.
In this study, we first detect synovial sarcoma stem cell and stem cell marker, and these findings play a very important role about exploitation the new therapy against synovial sarcoma.
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Report
(3 results)
Research Products
(50 results)
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[Journal Article] DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma.2010
Author(s)
Wang, L., Nishihara, H., Kato, Y., Kimura, T., Tanino, M., Nishio, M., Endo, T., Koike, T., Tanaka, S.
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Journal Title
Biochem. Biophys. Res.Commun. 395
Pages: 111-115
NAID
Related Report
Peer Reviewed
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[Journal Article] Analysis of an alternative human CD133 promoter reveals the implication of Ras/ERK pathway in tumor stem-like hallmarks.2010
Author(s)
Tabu, K., Kimura, T., Sasai, K., Wang, L., Bizen, N., Nishihara, H., Taga, T., Tanaka, S.
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Journal Title
Mol.Cancer. 9
Pages: 39-54
Related Report
Peer Reviewed
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[Journal Article] Sudden death of a patient with pandemic influenza (A/H1N1pdm) virus infection by acute respiratory distress syndrome.2010
Author(s)
Takiyama, A., Wang, L., Tanino, M., Kimura, T., Kawagishi, N., Kunieda, Y., Katano, H., Nakajima, N., Hasegawa, H., Takagi, T., Nishihara, H., Sata, T., Tanaka, S.
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Journal Title
Jpn.J.Infect.Dis. 63
Pages: 72-74
Related Report
Peer Reviewed
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[Journal Article] Colonic metastasis from primary carcinoma of the lung : report of a case and review of Japanese literature.2009
Author(s)
Ono, H., Okabe, M., Kimura, T., Kawakami, M., Nakamura, K., Dnajo, Y., Takasugi, H., Nishihara, H.
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Journal Title
Clin.J.Gastroenterol. 2
Pages: 89-95
Related Report
Peer Reviewed
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[Journal Article] Human synovial sarcoma proto-oncogene Syt is essential for early embyonic development through the regulation of cell migration.2009
Author(s)
Kimura, T., Sakai, M., Tabu, K., Wang, L., Tsunematsu, R., Tsuda, M., Sawa, H., Nagashima, K., Nishihara, H., Hatakeyama, S., Nakayama, K., Ladanyi, M., Tanaka, S., Nakayama, KI.
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Journal Title
Lab.Invest. 89
Pages: 645-656
Related Report
Peer Reviewed
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[Presentation] CrkL plays a pivotal role in tumoregenesis of the oral squamous cell carcinoma.2010
Author(s)
Hiroko, Nakamura, Hiroshi, Nishihara, Taichi, Kimura, Kei, Wang, Satoshi, Fukuda, Shinya Tanaka.
Organizer
第69回日本癌化学会学術総会
Place of Presentation
大阪国際会議場
Related Report
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