| Project/Area Number |
21791426
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital |
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Principal Investigator |
KONAGI Tanaka Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, 病態総合研究部, 研究員 (00393025)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 変形性関節症 / 血管内皮細胞増殖因子 |
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| Research Abstract |
The result of gene expression analysis in cartilage and clinical experience suggested possible involvement of VEGF-A in the pathology of osteoarthritis (OA). This study was conducted to examine the possibility of the involvement of VEGF-A in the synovial change observed with the disease. I first performed compression experiments with cartilage tissues. For this, cartilage tissues were obtained from OA and control knee joints, and compressive force was given to the tissues repeatedly to simulate weight bearing situation in the actual daily activities. The amount of pressure was adjusted to the level that cartilage tissues undergo in level walking (1 MPa). The result of this experiment revealed that cartilage tissues from OA knees release considerable amounts of VEGF-A upon physiological levels of compression. The amount of VEGF-A released from the tissue was significantly related to the severity of degeneration. Since this result suggested possible involvement of VEGF-A in the synovial changes occurs within OA joints, gene expression analysis was conducted with OA synovium. For this, synovial tissues were obtained from end-stage OA knees, and the levels of gene expression related to VEGF-A were determined by qPCR. However, the result of this analysis did not support our hypothesis that VEGF-A released from cartilage tissues induces synovial change. Thus, from the result of this study, it was concluded that VEGF-A might be released from degenerated cartilage, but it does not play a significant role in the pathology of OA, at least in terms of synovial pathology.
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