production of monoclonal antibodies against prostate cancer stem cells using differential dendritic cell immunization
| Project/Area Number |
21791532
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Nagasaki University |
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Principal Investigator |
TAMURA Takahiko Nagasaki University, 医歯薬学総合研究科, 助教 (00434035)
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| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 腫瘍学 / 抗体作製 / 樹状細胞 / 抗体 / 癌幹細胞 |
|---|
| Research Abstract |
The regulatory dendritic cells expressing HA antigen were immunized into mice. The anti-HA antibody was less induced compared with normal dendritic cells. However, OVA protein immunization after HA immunization demonstrated anti-OVA antibody was similarly induced, suggesting differential dendritic cell immunization protocol worked properly. Dendritic cells were shown to phagocyte the membrane proteins extracted from cultured cells. These dendritic cells were immunized into mice. Anti-cell surface proteins of culture cells were produced, but it was less in the case of regulatory dendritic cells.
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Report
(3 results)
Research Products
(6 results)
