Development and analysis of a mouse model of preeclampsia using transient in vivo gene transfer system

• Project/Area Number: 21791553
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka University
• Principal Investigator: KOYAMA Shinsuke Osaka University, 医学系研究科, 助教 (30528005)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 妊娠高血圧症候群 / 生体内遺伝子導入 / マウスモデル / ex vivo / 遺伝子導入

Research Abstract

Recently, "Two Step Theory" has received a lot of attention as pathological condition of preeclampsia, concretely speaking, reduced placental perfusion due to insufficient chorionic invasion to the uterus at early pregnancy is postulated as the root cause and maternal systemic endothelial dysfunction is induced by some over-expressed inhibitor of angiogenesis factor due to hypoxic status at placenta. In this research, we transferred TIMP-3 which is known as related factor of regulation of chorionic invasion to the decidua into the uterine cavity during implantation window, thus we successfully developed a mouse of preeclampsia.

Research Products

• [Journal Article] Pseudomyxoma peritonei originating from colorectal cancer during pregnancy. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Sawada, K., Kanagawa, T., Tsutsui, T., Kimura, T.
  • Journal Title: J Obstet Gynaecol Res 37(3) Pages: 254-258
• [Journal Article] Cervical insufficiency following cesarean delivery after prolonged second stage of labor : experiences of two cases. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Kanagawa, T., Sawada, K., Tsutsui, T., Kimura, T.
  • Journal Title: J Obstet Gynaecol Res 36(2) Pages: 411-413
  • NAID: 10031121630
• [Journal Article] Prenatal diagnosis of polysplenia syndrome associated with increased nuchal translucency. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Kanagawa, T., Morimoto, A., Kimura, T.
  • Journal Title: Prenat Diagn 30(10) Pages: 1005-1006
• [Journal Article] Spinal subarachnoid hematoma following spinal anesthesia in a patient with HELLP syndrome. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Kanagawa, T., Sawada, K., Tsutsui, T., KimuS., Murata, Y.
  • Journal Title: Int J Obstet Anesth 19(1) Pages: 87-91
• [Journal Article] A case of complete hydatidiform mole with coexistent fetus: conclusive diagnosis of androgenesis of the molar placenta by variation of paternal acrocentric short arms. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Sawada, K., Kanagwaw Isobe, A., Kinugasa, Y., Tsutsui, T., Kimura, T.
  • Journal Title: Am J Perinatol 27(2) Pages: 143-149
• [Journal Article] Two multipotential transcription factor, NF-kappaB and Stat-3, play critical and hierarchal roles for implantation (2010)
  • Author(s): Kimura, T., Ogita, K., Kumasawa, K., Koyama, S., Tsutsui, T., Nakamura, H.
  • Journal Title: Indian Journal of Physiology and Pharmacology Volume: 54(5) Pages: 27-32
  • Peer Reviewed
• [Journal Article] Prenatal diagnosis of polysplenia syndrome associated with increased nuchal translucency (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Kanagawa, T., Morimoto, A., Kimura, T.
  • Journal Title: Prenat Diagn Volume: 30(10) Pages: 1005-1006
  • Peer Reviewed
• [Journal Article] Cervical insufficiency following cesarean delivery after prolonged second stage of labor : experiences of two cases (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Kanagawa, T., Sawada, K., Tsutsui, T., Kimura, T.
  • Journal Title: J Obstet Gynaecol Res Volume: 36(2) Pages: 411-413
  • NAID: 10031121630
  • Peer Reviewed
• [Journal Article] Spinal subarachnoid hematoma following spinal anesthesia in a patient with HELLP syndrome (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Kanagawa, T., Sawada, K., Tsutsui, T., Kimura, T., Chang, YS., Wasada, K., Imai, S., Murata, Y.
  • Journal Title: Int J Obstet Anesth Volume: 19(1) Pages: 87-91
  • Peer Reviewed
• [Journal Article] Prenatal diagnosis of fetal intracranial hemorrhage in pregnancy complicated by idiopathic thrombocytopenic purpura (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Sawada, K., Kanagawa, T., Isobe, A., Taniguchi, Y., Wada, T., Kimura, T., Arahori, H., Kitabatake, Y., Wada, K.
  • Journal Title: Prenat Diagn Volume: 30(5) Pages: 489-491
  • Peer Reviewed
• [Journal Article] Pseudomyxoma peritonei originating from colorectal cancer during pregnancy. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Sawada, K., Kanagawa, T., Tsutsui, T., Kimura, T.
  • Journal Title: J Obstet Gynaecol Res Volume: 37(3) Pages: 254-258
  • Peer Reviewed
• [Journal Article] 妊娠高血圧症候群の病態解明-分子機構を中心に-着床障害モデルからみた妊娠高血圧症候群 (2010)
  • Author(s): 香山晋輔, 中村仁美, 張慶, 田畑知沙, 筒井建紀, 木村正
  • Journal Title: 産婦人科の実際 Volume: 59(7) Pages: 1035-1042
  • Peer Reviewed
• [Journal Article] A case of complete hydatidiform mole with coexistent fetus : conclusive diagnosis of androgenesis of the molar placenta by variation of paternal acrocentric short arms (2009)
  • Author(s): Koyama, S., Tomimatsu, T., Sawada, K., Kanagawa, T., Isobe, A., Kinugasa, Y., Tsutsui, T., Kimura, T.
  • Journal Title: Am J Perinatol. 27 Pages: 143-149
  • Peer Reviewed
• [Journal Article] Spinal subarachnoid hematoma following spinal anesthesia in a patient with HELLP syndrome. (2009)
  • Author(s): Koyama, S., Tomimatsu, T., Kanagawa, T., Sawada, K., Tsutsui, T., Kimura, T., Chang, YS., Wasada, K., Imai, S., Murata, Y.
  • Journal Title: Int J Obstet Anesth 19 Pages: 87-91
  • Peer Reviewed
• [Journal Article] 妊娠子宮局所におけるマクロファージの役割 (2009)
  • Author(s): 中村仁美、香山晋輔、谷口武、荻田和秀、熊澤恵一、張慶、筒井建紀、古山将康、杉野法広、田村博史、金田安史、木村正
  • Journal Title: 生殖内分泌学会雑誌 14巻 Pages: 35-40
  • Peer Reviewed
• [Presentation] 不育症に対する抗血小板療法/抗凝固療法に関するアンケート調査 (2011)
  • Author(s): 田畑知沙、筒井建紀、林正美、中村仁美、大八木知史、若林敦子、瀧内剛、正木秀武、筒井建紀、香山晋輔、木村正
  • Organizer: 第136回日本生殖医学会関西支部集団会第42回関西アンドロロジーカンファレンス
  • Place of Presentation: ハービスPLAZA(大阪市)
  • Year and Date: 2011-03-05
• [Presentation] Significance of feto-maternal interface : from implantation failure to preeclampsia in model mice. (2010)
  • Author(s): Kimura, T., Zhang, Q., Nakamura, H., Kumasawa, K., Koyama, S., Tabata, C., Tsutsui, T.
  • Organizer: 2nd International Conference on Reproductive Immunology.
  • Place of Presentation: Shanghai(招待講演)
  • Year and Date: 2010-10-02
• [Presentation] 胎児頭蓋内出血を来した特発性血小板減少性紫斑病(Idiopathic Thrombocytopen ic Purpura : ITP)合併妊娠の一症例 (2010)
  • Author(s): 香山晋輔, 朋和, 谷口友基子, 晶磯, 金川武司, 澤田健二郎, 富松拓治, 木村正
  • Organizer: 第62回日本産婦人科学会学術講演会
  • Place of Presentation: 東京都(東京國際フォーラム)
  • Year and Date: 2010-10-02
• [Presentation] Bizarre expression of tissue inhibitor of metalloproteinase-3 in feto-maternal interface caused gertational hypertension in mice model. (2010)
  • Author(s): Kimura, T., Zhang, Q., Nakamura, H., Kumasawa K, Koyama S., Tabata C., Tsutsui, T.
  • Organizer: XI International Congress of Reoroductive Immunology.
  • Place of Presentation: Cairns, AUSTRALIA
• [Presentation] 中隔子宮を有する挙児希望患者に対する子宮鏡下中隔子宮切除述後の妊娠予後 (2010)
  • Author(s): 筒井建紀, 香山晋輔, 田畑知沙, 横井猛, 木村正, 朝倉寛之
  • Organizer: 第50回日本産婦人科内視鏡学会学術講演会
  • Place of Presentation: 東京都(京王プラサセホテル)
• [Presentation] Mayer-Rokitansky-Kuster-Hauser syndromeに対して施行した腹腔鏡補助下造膣術(Cavydov変法)的検討 (2010)
  • Author(s): 瀧内剛, 香山晋輔, 横井猛, 金山智子, 筒井建紀, 木村正
  • Organizer: 第50回日本産婦人科内視鏡学会学術講演会
  • Place of Presentation: 東京都(京王プラサセホテル)
• [Presentation] 末梢血単球細胞を用いた着床子宮局所におけるマクロファージの体外での再構築 (2010)
  • Author(s): 田畑知沙, 中村仁美, 香山晋輔, 筒井建紀, 木村正
  • Organizer: 第62回日本産婦人科学会学術講演会
  • Place of Presentation: 東京都(東京国際フォーラム)
• [Presentation] 中隔子宮患者には、排卵障害が多いか? (2010)
  • Author(s): 筒井建紀, 香山晋輔, 田畑知沙, 朝野久美子, 中村仁美, 熊澤恵一, 前田真紀子, 根來英典, 木村正, 松下克子, 朝倉寛之
  • Organizer: 第62回日本産婦人科学会学術講演会
  • Place of Presentation: 東京都(東京国際フォーラム)
• [Presentation] 胎児頭蓋内出血を来たした特発性血小板減少性紫斑病(Idiopathic Thrombocytopenic Purpura:ITP)合併妊娠の一症例 (2010)
  • Author(s): 香山晋輔、和田朋、谷口友基子、磯部晶、金川武司、澤田健二朗、冨松拓治、木村正
  • Organizer: 第62回日本産婦人科学会学術講演会
  • Place of Presentation: 東京都(東京国際フォーラム)
• [Presentation] 動脈スティフネスの非侵襲的測定による高血圧症候群の早期診断の可能性 (2010)
  • Author(s): 藤目美佳、富松拓治、和田朋、谷口友基子、香山晋輔、磯部晶、金川武司、澤田健二郎、木村正、谷口武
  • Organizer: 第62回日本産婦人科学会学術講演会
  • Place of Presentation: 東京都(東京国際フォーラム)
• [Presentation] 妊娠中に発見された横行結腸癌由来の腹膜偽粘液腫の一症例 (2010)
  • Author(s): 佐々本尚子、香山晋輔、谷口友基子、磯部晶、金川武司、澤田健二郎、冨松拓治、瀧内剛、宮武崇、木村正
  • Organizer: 第62回日本産婦人科学会学術講演会
  • Place of Presentation: 東京都(東京国際フォーラム)
• [Presentation] 母体心停止後の胎児評価について～縊頸にて最低20分の母体心停止があった後に搬送・分娩となった1症例～ (2010)
  • Author(s): 和田朋、冨松拓治、谷口友礎子、香山晋輔、磯部晶、金川武司、澤田健二郎、木村正
  • Organizer: 第62回日本産婦人科学会学術講演会
  • Place of Presentation: 東京都(東京国際フォーラム)
• [Presentation] 当施設でのもやもや病合併妊娠の予後に関する-妊娠中に出血症状により初めて診断されたもやもや合併症妊娠の1例を通じて- (2010)
  • Author(s): 三宅達也、冨松拓治、谷口友基子、香山晋輔、磯部晶、金川武司、澤田健二郎、木村正、荻田和秀
  • Organizer: 第62回日本産婦人科学会学術講演会
  • Place of Presentation: 東京都(東京国際フォーラム)
• [Presentation] Mayer-Rokitansky-Kuster-Hauser syndromeに対して施行した腹腔鏡補助下造膣術(Davydov変法)の検討 (2010)
  • Author(s): 瀧内剛, 香山晋輔, 横井猛, 金山智子, 筒井建紀, 木村正
  • Organizer: 第50回日本産婦人科内視鏡学会学術講演会
  • Place of Presentation: 東京都(京王プラザホテル)
• [Presentation] 中隔子宮を有する挙児希望患者に対する子宮鏡下中隔子宮切除述後の妊娠予後 (2010)
  • Author(s): 筒井建紀, 香山晋輔, 田畑知沙, 横井猛, 木村正, 朝倉寛之
  • Organizer: 第50回日本産婦人科内視鏡学会学術講演会
  • Place of Presentation: 東京都(京王プラザホテル)
• [Presentation] Bizarre expression of tissue inhibitor of metalloproteinase-3 in feto-maternal interface caused gestational hypertension in mice model (2010)
  • Author(s): Kimura T, Zhang Q, Nakamura H, Kumasawa K, Koyama S, Tabata C, Tsutsui T
  • Organizer: XI International Congress of Reproductive Immunology
  • Place of Presentation: Cairns, AUSTRALIA