Analysis of mechanism deglycosilated IgAll produced by tonsillar lymphcyte deposits in the renal mesangium

Research Project

Project/Area Number	21791640
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Otorhinolaryngology
Research Institution	Kitasato University
Principal Investigator	ITOH Akihiko Kitasato University, 医学部, 非常勤講師 (10317026)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	扁桃 / IgA腎症 / 糖鎖不全 / ムチン型糖鎖 / マンノース / N-結合型糖鎖 / ヒンジ部 / 3次元マップ法 / シアル酸 / κカゼイン / ジャカリン / HAA / ヒンジ / ガラクトース欠損 / 糖鎖
Research Abstract	Recently, improved detection method of the de-galactosylated IgAl by enzymatic removal of sialic acid was developed and applied for the detection of the aberrant IgAl (algA) in IgA nephropathy. The removal of sialic acid from serum proteins was carried out by weak acid treatment. Consequently, sensitivity of the detection of a1gA was increased about hundred times. A tonsil may produce a1gA1 in serum because of overproduction of tonsillar algAl. Complete removal of sialic acid, did not increase sensitivity any more. Polymerization of algA must be most important factor for the sensitive detection of aIgA

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