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Three dimensional reconstruction of electronmicroscopic images by New electron microscopic tomography

Research Project

Project/Area Number 21791690
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionKyushu University

Principal Investigator

HISATOMI Toshio  Kyushu University, 医学研究院, 助教 (50404033)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords眼細胞生物学 / 電子顕微鏡 / 細胞死 / アポトーシス / 3次元再構築 / 電子線トモグラフィー
Research Abstract

The immunihistochemistry for electron microscopy was established for electron microscopic tomography. The automatic input of continuous serial images and 3D reconstruction from these images were examined for tomography.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (26 results)

All 2011 2010 2009 Other

All Journal Article (9 results) Presentation (16 results) Remarks (1 results)

  • [Journal Article] Utilizing Targeted Gene Therapy with Nanoparticles Binding Alpha v Beta 3 for Imaging and Treating Choroidal Neovascularization.2011

    • Author(s)
      Salehi-Had H, Roh MI, Giani A, Hisatomi T, Nakao S, Kim IK, Gragoudas ES, Vavvas D, Guccione S, Miller JW.
    • Journal Title

      PLoS One. 6(4)

    • Related Report
      2010 Final Research Report
  • [Journal Article] Receptor interacting protein kinases mediate retinal detachment-induced photoreceptor necrosis and compensate for inhibition of apoptosis.2010

    • Author(s)
      Trichonas G, Murakami Y, Thanos A, Morizane Y, Kayama M, Debouck CM, Hisatomi T, Miller JW, Vavvas DG.
    • Journal Title

      Proc Natl Acad Sci U S A. 107(50)

      Pages: 21695-21700

    • Related Report
      2010 Final Research Report
  • [Journal Article] Establishment of a new animal model of focal subretinal fibrosis that resembles disciform lesion in advanced age-related macular degeneration.2010

    • Author(s)
      Jo YJ, Sonoda KH, Oshima Y, Takeda A, Kohno R, Yamada J, Hamuro J, Yang Y, Notomi S, Hisatomi T, Ishibashi T.
    • Journal Title

      Invest Ophthalmol Vis Sci. 4

    • Related Report
      2010 Final Research Report
  • [Journal Article] Retinitis pigmentosa associated with asteroid hyalosis.2010

    • Author(s)
      Ikeda Y, Hisatomi T, Murakami Y, Miyazaki M, Kohno R, Takahashi H, Hata Y, Ishibashi T.
    • Journal Title

      Retina. 30(8)

      Pages: 1278-1281

    • Related Report
      2010 Final Research Report
  • [Journal Article] Histopathology of neovascular tissue from eyes with proliferative diabetic retinopathy after intravitreal bevacizumab injection.2010

    • Author(s)
      Kohno R, Hata Y, Mochizuki Y, Arita R, Kawahara S, Kita T, Miyazaki M, Hisatomi T, Ikeda Y, Aiello LP, Ishibashi T.
    • Journal Title

      Am J Ophthalmol. 150(2)

      Pages: 223-229

    • Related Report
      2010 Final Research Report
  • [Journal Article] An animal model of spontaneous metabolic syndrome : Nile grass rat.2010

    • Author(s)
      Noda K, Melhorn MI, Zandi S, Frimmel S, Tayyari F, Hisatomi T, Almulki L, Pronczuk A, Hayes KC, Hafezi-Moghadam A.
    • Journal Title

      FASEB J. 24(7)

      Pages: 2443-2453

    • Related Report
      2010 Final Research Report
  • [Journal Article] Localization of vascular adhesion protein-1 (VAP-1) in the human eye.2010

    • Author(s)
      Almulki L, Noda K, Nakao S, Hisatomi T, Thomas KL, Hafezi-Moghadam A.
    • Journal Title

      Exp Eye Res. 90(1)

      Pages: 26-32

    • Related Report
      2010 Final Research Report
  • [Journal Article] Pharmacological inhibition of mitochondrial membrane permeabilization for neuroprotection.2009

    • Author(s)
      Hisatomi T, Ishibashi T, Miller JW, Kroemer G.
    • Journal Title

      Exp Neurol. 218(2)

      Pages: 347-352

    • Related Report
      2010 Final Research Report
  • [Journal Article] Surprising up-regulation of P-selectin glycoprotein ligand-1(PSGL-1)in endotoxin-induced uveitis.2009

    • Author(s)
      Almulki L, Noda K, Amini R, Schering A, Garland RC, Nakao S, Nakazawa T, Hisatomi T, Thomas KL, Masli S, Hafezi-Moghadam A.
    • Journal Title

      FASEB J. 23(3)

      Pages: 929-939

    • Related Report
      2010 Final Research Report
  • [Presentation] マウス脈絡膜新生血管の蛍光走査型電子顕微鏡(FL-SEM)による観察2011

    • Author(s)
      納富昭司・久冨智朗
    • Organizer
      日本眼科学会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-04-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Brilliant Blue Gの細胞染色性と細胞膜透過性の検討2011

    • Author(s)
      久冨智朗
    • Organizer
      班会議
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] Brilliant Blue Gの細胞染色性と細胞膜透過性の検討2011

    • Author(s)
      久冨智朗
    • Organizer
      日本眼科学会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] P2X7受容体遮断薬Brilliant Blue Gによる網膜神経細胞保護効果の検討2011

    • Author(s)
      納富昭司、久冨智朗
    • Organizer
      班会議
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] P2X7受容体遮断薬Brilliant Blue Gによる網膜神経細胞保護効果の検討2011

    • Author(s)
      納富昭司、久冨智朗
    • Organizer
      日本眼科学会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] 胞状網膜剥離と浅い網膜剥離における視細胞死の比較2010

    • Author(s)
      久冨智朗
    • Organizer
      分子生物学研究会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] 胞状網膜剥離と浅い網膜剥離における視細胞死の比較2010

    • Author(s)
      久冨智朗
    • Organizer
      日本眼科学会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] マウス脈絡膜新生血管の蛍光走査型電子顕微鏡(FL-SEM)による観察2010

    • Author(s)
      納富昭司、久冨智朗
    • Organizer
      分子生物学研究会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] マウス脈絡膜新生血管の蛍光走査型電子顕微鏡(FL-SEM)による観察2010

    • Author(s)
      納富昭司、久冨智朗
    • Organizer
      日本眼科学会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] 網膜剥離による視細胞死;ミトコンドリア脂質二重膜透過性亢進をした分子機構2009

    • Author(s)
      久冨智朗
    • Organizer
      若手神経保護研究会
    • Place of Presentation
      東京
    • Year and Date
      2009-09-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 視細胞保護と血管新生抑制におけるアポトーシスの関与2009

    • Author(s)
      久冨智朗、納富昭司
    • Organizer
      眼創傷治癒研究会
    • Place of Presentation
      仙台
    • Year and Date
      2009-08-07
    • Related Report
      2010 Final Research Report
  • [Presentation] 網膜剥離による視細胞死;ミトコンドリア脂質二重膜透過性亢進を介した分子機構2009

    • Author(s)
      久冨智朗
    • Organizer
      眼分子生物学研究会
    • Place of Presentation
      福岡
    • Related Report
      2010 Final Research Report
  • [Presentation] 網膜剥離と視細胞死;ミトコンドリア透過性亢進を介した分子機構2009

    • Author(s)
      久冨智朗
    • Organizer
      北九州九州大学眼科同門会
    • Place of Presentation
      小倉
    • Related Report
      2010 Final Research Report
  • [Presentation] 眼科血管・リンパ管研究の最前線 実験的脈絡膜血管新生の発生および退縮過程 におけるアポトーシスの発生と役割(シンポジウム)2009

    • Author(s)
      久冨智朗
    • Organizer
      日本眼科学会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] 網膜剥離でなぜ視細胞は変性するのか? ミトコンドリアの謎(インストラクションコース)2009

    • Author(s)
      久冨智朗
    • Organizer
      日本臨床眼科学会
    • Place of Presentation
      福岡
    • Related Report
      2010 Final Research Report
  • [Presentation] The Bird's Eye View Analysis of Internal limiting membrane ; Cellular migration around the macular hole2009

    • Author(s)
      Toshio Hisatomi
    • Organizer
      JCK meeting
    • Place of Presentation
      Fukuoka
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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