Investigation of neural crest stem cell therapy and niche factors for Hirschsprung's disease
Project/Area Number |
21791736
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Pediatric surgery
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Research Institution | Keio University |
Principal Investigator |
NISHIKAWA Ryuhei Keio University, 医学部, 助教 (30534531)
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Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | neural crest-derived stem cells NCSCs / Hirschsprung's disease / Neurosphere-like Bodies NLBs / Ret / 移植 / 再生医療 / ヒルシュスプルング病 / ニッチ因子 / 腸管神経系 / protein zero protein / c-Ret受容体 / stem cells / glial cell line-derived neurotrophic Factor |
Research Abstract |
Hirschsprung's disease, also called aganglionosis or megacolon, is a developmental disorder of large intestine (colon) which occurs in 1 in 5000 children. Ret KO (Ret^<[-/-]>) mouse were one of the Hirschsprung's disease model mouse which have no intestinal nerve systems. An object of this NLB co-culture study with Ret^<[-/-]>KO mouse aganglionic colon is to research regenerative medicine tactics for Hirschsprung's disease using de novo Neurosphere-like Bodies (NLBs) generation protocol with serum free culture to regenerate enteric nervous system. Although there are several report described Generation of NLBs in gut but serum free culture procedure have not reported. P0-Cre/floxed-EGFP mice are known to be used to target neural crest derived stem cells (NCSCs) population using EGFP fluorescent and could separate NCSCs rich cell population using FACS cell sorter. We generated Neurosphere-like Bodies (NLBs) from the E13.5 embryo gut of transgenic mice P0-Cre/floxp-EGFP and then cells were cultured in the serum-free medium successfully. After 10 days to 3 w cultures, serum-Free-cultured EGFP-positive cells formed Neurosphere-like structures that expressed NCSC genes. Neurons, glial cells, and myofibroblasts marker protein expression were confirmed after 4days culture of NLBs using poly-D-lysine-laminin coated slide chamber and 10% FBS contained medium.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] ヒルシュスプルング病の神経堤幹細胞移植治療に必要とされるニッチ因子の研究2009
Author(s)
西川竜平, 下島, 直樹, 森川, 康英, 森, 昌玄, 山本, 裕輝, 山田, 洋平, 堀田, 亮, 渕本, 康史, 星野, 健, 名越, 慈人, 中村, 雅也, 芝田, 晋介, 岡野, ジェイムス洋尚, 岡野, 栄之
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Journal Title
日本小児外科学会雑誌 第45巻5号
Pages: 884-884
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