|Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Cryopreservation of biological tissue needs special facilities. Vitrification procedure may solve this problem. It is known that vitrification procedure increases cryopreserved cell survival rate. We carried out cryopreservation and dried preservation at room temperature of human and rat skin by vitrification procedure. Besides, we carried out rat skin grafting. We could confirm cell structure maintain by transmission electron microscopy, but cells could not survive.