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The roles of bacterial lipoproteins on bacterial invasion and on intracellular trafficking in host cells

Research Project

Project/Area Number 21791780
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionHokkaido University

Principal Investigator

HASEBE Akira  Hokkaido University, 大学院・歯学研究科, 助教 (90281815)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords口腔細菌学 / リポタンパク質 / 付着因子 / TLR2 / FSL-1 / クラスリン依存的 / CD14 / CD36
Research Abstract

To study the roles of bacterial lipoproteins on bacterial invasion to host cells and on the effects on intracellular trafficking in host cells, attempts were made to purify a mycoplasmal lipoprotein, since some mycoplasmas are known to be able to invade into host cells. In this study, a lipoprotein was purified from M. hominis. The 40 kDa-lipoprotein was purified, and its N-terminal amino acid sequence was identical to that of P50 lipoprotein, which had already been reported as an adhesin of M. hominis. Interestingly, this lipoprotein could adhere to THP-1 cells but anti TLR2 antibody failed to inhibit adherence of this lipoprotein to THP-1 cells. We are now under preparation for submitting these results to an international journal on immunology.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (5 results)

All 2010 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] The Toll-like receptor 2 (TLR2) ligand FSL-1 is internalized via the clathrin-dependent endocytic pathway triggered by CD14 and CD36 but not by TLR2.2010

    • Author(s)
      Haque M.Shamsul, Akira Hasebe, Mitsuhiro Iyori, Makoto Ohtani, Kazuto Kiura, Diya Zhang, Yasunori Totsuka, Ken-ichiro Shibata.
    • Journal Title

      Immunology. 130

      Pages: 262-272

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Toll-like receptor 2(TLR2)ligand FSL-1 is internalized via the clathrin-dependent endocytic pathway triggered by CD14 and CD36 but not by TLR22010

    • Author(s)
      Shamsul HM, Hasebe A, et al.
    • Journal Title

      Immunology (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] The Toll-like receptor 2(TLR2)ligand FSL-1 is internalized via the clathrin-dependent endocytic pathway triggered by CD14 and CD36 but not by TLR22010

    • Author(s)
      Akira Hasebe, Ken-ichiro Shibata
    • Organizer
      第10回日韓国際微生物学シンポジウム
    • Place of Presentation
      パシフィコ横浜, 横浜
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光線力学療法応用による培養細胞からのマイコプラズマ除去2010

    • Author(s)
      長谷部晃, et al.
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      タワーホール船堀(東京)
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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